Báo cáo khoa học: The Janus-faced atracotoxins are speciﬁc blockers of invertebrate KCa channels ppt

... as these other toxins. This is probablydue to the bifurcated d-guanidinium group at the tip of the critical Arg8 residue, which is much bulkier than the single amine moiety at the tip of the ... Journal 275 (2008) 4045–4059 ª 2008 The Authors Journal compilation ª 2008 FEBS 4059 The Janus-faced atracotoxins are speciﬁc blockers of invertebrate KCa channels Simon J. Gunning1, Francesco ... June2008)doi:10.1111/j.1742-4658.2008.06545.x The Janus-faced atracotoxins are a unique family of excitatory peptidetoxins that contain a rare vicinal disulﬁde bridge. Although lethal to a widerange of invertebrates, their molecular...

Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

... However, neither the speciﬁcity of bacitracin for PDI nor the detailed mech-anisms of inhibition of PDI have been investigated.Furthermore, since the original reporting of the inhibi-tion of PDI ... mechanism of action, the reaction conditions of the assay were var-ied. The removal of EDTA from the reaction signiﬁ-cantly reduced the noncatalyzed rate (Fig. 2C;P < 0.05) and the catalysis of ... folding. Analysis of the nonassisted refolding of rhodanese showed the expected aggregation of the folding intermediates. The addition of PDI or the noncatalytic PDI family memberERp27 to the refolding...

Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc

... andPLC are capable of rapidly elevating the level of DAG. Inagreement with these reports, we found that the addition of 100 lMATP doubled DAG mass within 5 min; interest-ingly, the presence of ... volume of 55 lL. For the speciﬁc measurement of the atypical PKCisoenzyme-f,Ca2+was omitted from the assay, 80 lgÆmL)1phosphatidylserine was substituted for the kit lipid reagentand the kit ... treat-ment of the cells did not alter the amount of PKCf in the immunoprecipitate.ResultsHepatocytes used in the present study were routinelycultured serum-free in the presence of 0.1 lMdexametha-sone...

Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identiﬁcation of new components and synergies between complexes ppt

... proteins; Mpep, the number of unique matched peptides; Upep, the number of unmatched peptides in the MALDI-TOF experiments. The function of new proteins is based on the GH family of the catalytic ... half the activity of F5. All in all, these resultsindicate that the differences in the protein composition of the cellulosomes are related to different enzymaticproperties.Identiﬁcation of new ... activi-ties of the individual fractions measured independently (at half orone third of the protein concentration). The dark grey bars indicate the activity of the control. Synergy values are indicated...

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... encoded another type of KLK11, isoform 2,despite the presence of exon 1c. The secretory path-ways of KLK11 isoforms appeared to differ, althoughboth isoforms were secreted from cells. Hence, the ... raised against KLK11 was the same sizeas that of the translational product by isoform 2mRNA (Fig. 4B). The product by isoform 1 mRNAwas smaller than the isoform 2 product. The multiplebands may ... sites of KLK11 because it was alreadyreported that mRNAs for both isoforms 1 and 2 are detected in the organ. All of these nucleotide sequenceswere identical to the genomic sequence of the humanKLK11...

Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx

... Many diseases are associated with changes to sHSPs occurring either as a result of mutation or by post-translational changes, and these are outlined below and described in the text of the review. ... actin ﬁlament modiﬁcations. Synthesis of exogenous human aA-crystallin in lens epithelial cells of the same species counters UVB-induced apoptosisby favoring action of the AKT kinase pathway, poten-tially ... Among the three cancers, sarcomacells possess the most Hsp25, the rodent equivalent of human Hsp27, and the protein builds up upon drugtreatment, suggestive of a role in cell survival. Inanother...

Báo cáo khoa học: Gene expression silencing with ‘speciﬁc’ small interfering RNA goes beyond speciﬁcity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx

... and refer-ence samples. The presence of the internal standard probesat different locations of the array allowed quantiﬁcation of the local background and evaluation of the array homoge-neity, ... into account in the normalization.Furthermore, in order to consider the purity and quality of the mRNA, a second normalization step was performed on the basis of the average of ﬂuorescence intensities ... the degradation of a particular mRNA (slicing) or to repression of translation [2,3]. Thereafter, the use of chemicallysynthesized siRNAs as a new loss -of- function strategyexploded during the...

Báo cáo khoa học: The 2-oxoacid dehydrogenase multi-enzyme complex of the archaeon Thermoplasma acidophilum ) recombinant expression, assembly and characterization docx

... with the substrate specificityobserved for the recombinant E1 enzyme in the absence of the other complex components [14]. The relative activities are given in Table 1, along with thosefor the ... using the programSEDFIT, and the data were then directly fitted using the finite element solution of the Lamm equation inSEDFIT [18] to givevalues of the sedimentation coefficient in the buffer of ... rec-ognize the lipoyl-domain of the Thermoplasma E2enzyme, the target lysine of which is flanked by D and Vresidues, as it is in the E2 protein of the E. coli OGDHCand of the Bacillus subtilis BCOADHC...

Báo cáo khoa học: The bI/bIII-tubulin isoforms and their complexes with antimitotic agents pptx

... superposition of the backbone atoms of ligand binding residues.These are deﬁned as the residues within 4 A˚ of anyatom of either paclitaxel in the 1JFF structure or epo-thilone in 1TVK. They are: ... compar-ison of the complete structures and of the most rele-vant parts of the structures (dimer interface andbinding site). The differences in the overall structure are rather high in terms of rmsd ... Glu27.Differently from the interactions described above, the latter hydrogen bonds are less stable: in fact, due to the high mobility of the lateral chain of Glu27 they are observed in most of the sampled...

Báo cáo khoa học: The human cytomegalovirus-encoded chemokine receptor US28 induces caspase-dependent apoptosis pptx

... raises the question of whether this represents anexception or rather a particular example of a generalstrategy of viral subversion of chemokine receptorfunctions. It should be noted that proof of ... Research, Division of Medicinal Chemistry, the Netherlands3 Department of Medical Microbiology, University Hospital of Maastricht, the Netherlands4 Institute of Biochemistry, University of Lausanne, ... ﬂuorescence (y-axis). The percentages of living GFP + cells are indicated on the right and the percentages of apoptotic GFP + cells on the left.Fig. S2. Fluorescent microscope analysis of GFP andGFP-fused...

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