... double
deletion of the ppiD and surA genes did not result in
loss of viability.
To investigate how these PPIases interact with their
substrates and whether they have potentially overlap-
ping substrate ... SurA,
indicating that the two PPIases might interact with different substrates. We
therefore propose that, although PpiD and SurA have partially overlapping
s...
... conditions;
(b) the different shapes of the immature and the mature
HIV-1 capsid [32], and of the mature capsids of different
retroviruses; (c) the different structural organization of
CA in the immature and ... membrane, and the remaining
domains forming the innermost protein layers accor-
ding to their positions in Gag: MA, CA-NTD,
CA-CTD, the spacer peptide SP1, N...
... than 17 A
˚
apart from the exocyclic
methylene C-atom of the MIO prosthetic group.
On the basis of the experimental structures, hypothe-
ses on the role of the Tyr110-loop have been put for-
ward. ... from the PAL-
based models. Merging the MIO portions to the mono-
mers and building the ‘Tyr-loop-out’ tetrameric models
(PlPAL
out
and SmPAL
out
) was achieved in the...
... on the dynamics of the cytoskeleton,
the former promoting the recruitment of elements of
the Rac1 signaling pathway, and the latter regulating
Rho activity [47–50]. However, investigating whether
the ... the bands
was then determined by densitometry. Plots of the con-
centration of MBP-DHPH2 in the pellets versus total
MBP-DHPH2 protein added to the reaction mixture
ar...
... single band on t hese gels, as the ODN h ybridizes to the
center of the input target RNA. The asterisk indicates the input target
RNA; the arrow indicates the digested product bands. The amount ... levels of either
RNase H1 or H2. The discrepancy between the in vivo and
in vitro measurements could be due to a compartmentaliza-
tion of a component in the in vivo system....
... EGFP–AtRibF2
[43]. The hypothesis for the localization of FADS
(AtRibF1 and AtRibF2) isoforms in mitochondria
cannot, moreover, be ruled out on the basis of bioin-
formatics (see TAIR). Whether and how ... in the mitochondrial fraction
than in protoplasts (Fig. 1).
The mitochondrial and the extramitochondrial
amounts of Rf, FMN and FAD in the acid-extractable
fractions w...
... DnaJs lack the cysteine
repeats and type III DnaJs lack both the G ⁄ F region
and the cysteine repeats. Type I and Type II proteins
seem to have similar functions and they bind non-
native substrates, ... antigen which
included the J domain, the Rb-binding domain and the
DNA-binding domain, but lacked the ATPase domain
of the C-terminus. Given that the ATPase dom...
... masses
and the calculated ones of the recombinant
proteins were aligned in the table. (B) The
sequence of the synthetic 36- amino acid
peptide around the reactive site (underlined)
and the mass ... suggesting that the inhibi-
tion was temporary.
To identify the reactive site of the inhibitor, the P4
fragment was incubated with furin and the reaction
was stopped a...
... 14-3-3 g and c isoforms bind most tightly to both
the phospho-peptide and the dephosphopeptide and
have been identified both in vitro and in vivo as bind-
ing most strongly to CKIa (Figs 1–3). The ... Therefore, the difference in
phosphorylation dependence of the interactions that
we have demonstrated in the present study has impor-
tant implications for the respectiv...
... each condition. The box embraces the 25th and
75th percentile values. Whiskers extend from each end of
the box to the minimum and maximum values in the data.
Crosses represent the mean value.
Bio-informatics ... 71. Considering the location of the
N-terminal extremity of DNter2 on the structure of
CARP and the presence of the potential cleavage sites,
we identified...