Báo cáo khoa học: The periplasmic peptidyl prolylcis–transisomerases PpiD and SurA have partially overlapping substrate specificities doc

Báo cáo khoa học: The periplasmic peptidyl prolylcis–transisomerases PpiD and SurA have partially overlapping substrate specificities doc

Báo cáo khoa học: The periplasmic peptidyl prolylcis–transisomerases PpiD and SurA have partially overlapping substrate specificities doc

... double deletion of the ppiD and surA genes did not result in loss of viability. To investigate how these PPIases interact with their substrates and whether they have potentially overlap- ping substrate ... SurA, indicating that the two PPIases might interact with different substrates. We therefore propose that, although PpiD and SurA have partially overlapping s...
Ngày tải lên : 16/03/2014, 06:20
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Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: intersubunit interactions during virus assembly doc

Tài liệu Báo cáo khoa học: The capsid protein of human immunodeficiency virus: intersubunit interactions during virus assembly doc

... conditions; (b) the different shapes of the immature and the mature HIV-1 capsid [32], and of the mature capsids of different retroviruses; (c) the different structural organization of CA in the immature and ... membrane, and the remaining domains forming the innermost protein layers accor- ding to their positions in Gag: MA, CA-NTD, CA-CTD, the spacer peptide SP1, N...
Ngày tải lên : 18/02/2014, 06:20
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Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx

Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx

... than 17 A ˚ apart from the exocyclic methylene C-atom of the MIO prosthetic group. On the basis of the experimental structures, hypothe- ses on the role of the Tyr110-loop have been put for- ward. ... from the PAL- based models. Merging the MIO portions to the mono- mers and building the ‘Tyr-loop-out’ tetrameric models (PlPAL out and SmPAL out ) was achieved in the...
Ngày tải lên : 07/03/2014, 12:20
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Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx

Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx

... on the dynamics of the cytoskeleton, the former promoting the recruitment of elements of the Rac1 signaling pathway, and the latter regulating Rho activity [47–50]. However, investigating whether the ... the bands was then determined by densitometry. Plots of the con- centration of MBP-DHPH2 in the pellets versus total MBP-DHPH2 protein added to the reaction mixture ar...
Ngày tải lên : 07/03/2014, 12:20
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Báo cáo khoa học: The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides pot

Báo cáo khoa học: The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides pot

... single band on t hese gels, as the ODN h ybridizes to the center of the input target RNA. The asterisk indicates the input target RNA; the arrow indicates the digested product bands. The amount ... levels of either RNase H1 or H2. The discrepancy between the in vivo and in vitro measurements could be due to a compartmentaliza- tion of a component in the in vivo system....
Ngày tải lên : 08/03/2014, 10:20
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Báo cáo khoa học: The occurrence of riboflavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status docx

Báo cáo khoa học: The occurrence of riboflavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status docx

... EGFP–AtRibF2 [43]. The hypothesis for the localization of FADS (AtRibF1 and AtRibF2) isoforms in mitochondria cannot, moreover, be ruled out on the basis of bioin- formatics (see TAIR). Whether and how ... in the mitochondrial fraction than in protoplasts (Fig. 1). The mitochondrial and the extramitochondrial amounts of Rf, FMN and FAD in the acid-extractable fractions w...
Ngày tải lên : 16/03/2014, 04:20
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Báo cáo khoa học: The viral SV40 T antigen cooperates with dj2 to enhance hsc70 chaperone function docx

Báo cáo khoa học: The viral SV40 T antigen cooperates with dj2 to enhance hsc70 chaperone function docx

... DnaJs lack the cysteine repeats and type III DnaJs lack both the G ⁄ F region and the cysteine repeats. Type I and Type II proteins seem to have similar functions and they bind non- native substrates, ... antigen which included the J domain, the Rb-binding domain and the DNA-binding domain, but lacked the ATPase domain of the C-terminus. Given that the ATPase dom...
Ngày tải lên : 16/03/2014, 05:20
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Báo cáo khoa học: The potent inhibitory activity of histone H1.2 C-terminal fragments on furin doc

Báo cáo khoa học: The potent inhibitory activity of histone H1.2 C-terminal fragments on furin doc

... masses and the calculated ones of the recombinant proteins were aligned in the table. (B) The sequence of the synthetic 36- amino acid peptide around the reactive site (underlined) and the mass ... suggesting that the inhibi- tion was temporary. To identify the reactive site of the inhibitor, the P4 fragment was incubated with furin and the reaction was stopped a...
Ngày tải lên : 16/03/2014, 13:20
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Báo cáo khoa học: The interaction between casein kinase Ia and 14-3-3 is phosphorylation dependent pot

Báo cáo khoa học: The interaction between casein kinase Ia and 14-3-3 is phosphorylation dependent pot

... 14-3-3 g and c isoforms bind most tightly to both the phospho-peptide and the dephosphopeptide and have been identified both in vitro and in vivo as bind- ing most strongly to CKIa (Figs 1–3). The ... Therefore, the difference in phosphorylation dependence of the interactions that we have demonstrated in the present study has impor- tant implications for the respectiv...
Ngày tải lên : 23/03/2014, 04:20
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Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf

Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf

... each condition. The box embraces the 25th and 75th percentile values. Whiskers extend from each end of the box to the minimum and maximum values in the data. Crosses represent the mean value. Bio-informatics ... 71. Considering the location of the N-terminal extremity of DNter2 on the structure of CARP and the presence of the potential cleavage sites, we identified...
Ngày tải lên : 29/03/2014, 21:20
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