Báo cáo khoa học: Structural studies of nucleoside analog and feedback inhibitor binding to Drosophila melanogaster multisubstrate deoxyribonucleoside kinase doc
... nucleoside analog and feedback
inhibitor binding to Drosophila melanogaster
multisubstrate deoxyribonucleoside kinase
Nils E. Mikkelsen
1
, Birgitte Munch-Petersen
2
and Hans Eklund
1
1 Department of ... structures of dNK complexed with four dif-
ferent nucleoside analogs (floxuridine, brivudine, zidovudine and zalcita-
bine) and relate them to the bindin...
... of rMIH-B at the arthropodial
membrane of the periopod and returned to the culture
tanks. At 24, 48 and 72 h after injection, the hepatopan-
creas and ovary of the shrimp were dissected for total
RNA ... monodon. As there is a high degree
of sequence similarity and structural conservation of
these genes and gene products, confirmation of the true
identity and functi...
... amount of oligomers, and this effect was used to investigate the
details of both oligomer formation and structure by a combination of bio-
physical techniques. The structural properties of the ... distribu-
tion of 100 l
M proinsulin C-peptide in the
presence of divalent Ca
2+
and Mg
2+
ions
under native conditions (B), and of 100 l
M
C-peptide in the presence of i...
... case of TIMs from
Plasmodium and yeast. Interestingly, the thioether group
of M103 is positioned to make a potentially stabilizing
contact with the guanidinium group of R99 (equivalent
to R98 of ... (equivalent
to R98 of PfTIM and yeast TIM). The shortest distance
from the sulfur atom of M103 to the NH1 nitrogen of
the guanidium group of R99 is 3.23 A
˚
, suggestiv...
... investi-
gated for the multisubstrate Drosophila melanogaster deoxyribonucleoside
kinase. Mutation of E52 to D, Q and H plus mutations of R105 to K and
H were performed to investigate the proposed ... Munch-Petersen B & Eklund H
(2003) Structural basis for feedback inhibition of the
deoxyribonucleoside salvage pathway: studies of the
Drosophila deoxyr...
... Norwegian Structural Biology Centre, Faculty of Science, University of Tromsø, Norway
2 Department of Molecular Biotechnology, Faculty of Medicine, University of Tromsø, Norway
The marine and estuarine ... of VsEndA (Fig. 1). The
theoretical pI was 9.61 for VsEndA and 8.62 for
VcEndA.
Expression and purification
From 7 L Escherichia coli culture, a total of 24 and
50 mg...
... consisting of four copies of four
subunits, a, b, c and d. The a and b subunits are regu-
latory and are the sites of phosphorylation and meta-
bolite binding and are also regulated by the binding
of ... II and IV (CaMKI, CaMKII and
CaMKIV, respectively), CaM kinase kinase (CaMKK),
titin kinase, and death associated kinase [4]. Structural
studies on CaMKI...
... L. Resnikoff and J. L. Dolby, "The Nature of
Affixing in Written English," an algorithm for the structural definition of
affixes was developed and applied to data consisting of all ...
pected to show up in two-vowel-string words. This paper summarizes the
results of applying a modified form of the operational definition to data
consisting of all the four...
... (the senses of GRASP in " ;to grasp a
baseball" and " ;to grasp an idea") or semantically
unrelated (e.g., the meanings of TIRE related to
"sleeplng" and "wheel"). ... initial access of meaning.
Ne suggested such contexts Rrlme one reading of the
ambiguous word, in the sense of Collins and Loftus
(1975), Meyer and Schvaneveldt...
... 1420
1409.04
C49
C51
C52
C53
C48
C50
C55
Fig. 3. Triacylated structures of N-terminal peptides of SitC of other
three S. aureus strains. MALDI-TOF mass spectra of the organic
phase of in-gel tryptic digests of SitC protein, which was isolated
through ... of SA2202 from
the SA113 strain were prepared and analyzed by
MALDI-TOF MS. As shown in Table 2, characteristic
peaks corres...