Báo cáo khoa học: A short proregion of trialysin, a pore-forming protein of Triatoma infestans salivary glands, controls activity by folding the N-terminal lytic motif pdf

... A short proregion of trialysin, a pore-forming protein of Triatoma infestans salivary glands, controls activity by folding the N-terminal lytic motif Rafael M. Martins1, Rogerio Amino2, ... 465–472.5 Amino R, Porto RM, Chammas R, Egami MI &Schenkman S (1998) Identiﬁcation and characterization of a sialidase released by the salivary gland of the hematophagous insect Triatoma infestans. ... R, Tanaka AS & Schenkman S (2001) Triapsin,an unusual activatable serine protease from the saliva of the hematophagous vector of Chagas’ disease Tria-toma infestans (Hemiptera: Reduviidae)....

Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx

... simpliﬁcation of the set of equations by assuming a steady state of the intra-cellular metabolites. An approach that combines ﬂuxbalance analysis (FBA) with an ordinary differentialequation ... by a process called inducer exclusion,whereas phosphorylated EIIA activates adenylatecyclase (CyaA) and leads to an increase in the intra-cellular cyclic AMP (cAMP) level [1].Mathematical ... design of bacterial strainsbased on mathematical models.Nishio et al. [15] provided simulation data of theirmodel and discussed the agreement with literatureexperimental data from a qualitative...

Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... actin-binding domainThirumaran Thanabalu1,2, Rajamuthiah Rajmohan2, Lei Meng2, Gang Ren4,5, Parimala R. Vajjhala4and Alan L. Munn1,3,4,6*1 Institute of Molecular and Cell Biology, A* STAR Biomedical ... importance of WASP–WIP interaction in mammalian cells is sup-ported by the observation that the vast majority of the disease-causing missense mutations in human WASPmap to the N-terminal WH1 ... and growth atelevated temperature, on the one hand, and in corticalactin-patch polarization, on the other hand, are at leastpartially distinct [23]. However, there may still be a functional...

Tài liệu Báo cáo khoa học: Aldehydes release zinc from proteins. A pathway from oxidative stress⁄lipid peroxidation to cellular functions of zinc pptx

... disulﬁram. Alcohol Alcohol 28,461–468.30 Isse T, Oyama T, Kitagawa K, Matsuno K, Matsumoto A, Yoshida A, Nakayama K, Nakayama K & Kawa-moto T (2002) Diminished alcohol preference in trans-genic ... exception of formaldehyde, none of the aldehydes affects the absorbance of PAR. The data for the reaction of MT with formalde-hyde were corrected for the absorbance changes in the absence of MT.Thiol ... release, the E-amino groups of lysines in MT2 were carbamoylated with potassiumcyanate and the modiﬁed protein was assayed for zincrelease as described above. Acetaldehyde releasesalmost the...

Tài liệu Báo cáo khoa học: Deciphering enzymes Genetic selection as a probe of structure and mechanism docx

... [38]. A transition state analog, shownin a ball-and-stick representation, is bound in each of the active sites,which are located at the trimer interfaces. The location of the cystal-lographically ... better anticipate the naturalevolution of bacterial antibiotic resistance. Hydrolysis of aztreonam requires a change in the substrate speciﬁcity of TEM-1 b-lactamase. The chance of ﬁnding the threemutations ... Binarypatterning [17] and restricted amino acid sets [18,22] canproduce proteins capable of folding. What is the smallestset of amino acids that can still produce a catalyticallycompetent protein? ...

Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identiﬁcation of oscillophores ppt

... removal. It is obtained for allsubstrates of any elementary reaction. The main point of the present section is that any Jacobianmatrix element equals the sum of a number of direct parallelinfluence ... influence paths) in the kineticscheme, i.e. the sum of paths through reaction-centeredgraphs of the type o f Graph 1 (these paths may containparallel and antiparallel arrows). The sign of that elementtherefore ... define the value of a subgraph that contains p reactions and p concentrations, asminus the sum of all its combined autoinfluence paths of order p. Note therefore that negativity of a subgraph andpositivity...

Tài liệu Báo cáo khoa học: Molecular cloning, recombinant expression and IgE-binding epitope of x-5 gliadin, a major allergen in wheat-dependent exercise-induced anaphylaxis ppt

... ¢-AAGTGAGCAATAGTAAACACAAATCAAAC-3¢and 5¢-CGTTACATTATGCTCCATTGACTAACAACGATG-3¢, were constructed based on fragment DNA sequences of the x-5 gliadin gene (GenBank accession numbersBE590673 and ... puriﬁcation of recombinant protein Sense (5¢-ATTTCATATGCAACAACAATTCCCCCAGCAACAATCA-3¢) and antisense (5¢-TCTCGGATCCTCATAGGCCACTGATACTTATAACGTCGCTCCC-3¢) oligo-nucleotide primers having an initiation ... this half of the x- 5a gliadin protein by PCR, the DNA fragment wasinserted into the expression vector pET-2 1a. E. coliRosetta (DE3) was used as a host strain as the x- 5a gene has a lot of rare...

Tài liệu Báo cáo khoa học: "Exploiting Readymades in Linguistic Creativity: A System Demonstration of the Jigsaw Bard" docx

... combinations than the averagehuman. The necessary barrage of linguistic stimulican be provided by the Google 1T database of Webngrams (Brants and Franz, 2006). Trawling thesengrams, a modestly ... orrestaurant names, or the names of racehorses, andso on, and on. The 1996 movie The Usual Suspectstakes its name from a memorable scene in 1942’sCasablanca, as does the Woody Allen play andmovie ... shall showthat the notion of a linguistic readymade has sig-nificant practical merit in the realms of text gen-eration and computational creativity.2 Linguistic ReadymadesReadymades are the...

Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf

... Uppsala, SwedenFax: +46 18 536971Tel: +46 18 4714177E-mail: maria.selmer@icm.uu.seDatabase The atomic coordinates and observedstructure factors are available in the Protein Data Bank database ... conformational changes in the eukaryotic ribosomal translocase. Nat Struct Biol10,379–385.30 Al Karadaghi S, Aevarsson A, Garber M, Zheltonos-ova J & Liljas A (1996) The structure of elongationfactor ... 666 and 670, within one b-strand of domain V, are also important for interdomain interac-tions. The N-terminal part of the strand interacts with the N-terminal part of helix CGin domain G, and...

Báo cáo khoa học: Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase l isoform pdf

... encoding at least nine splice variants, as wellas biochemical characterization of the canonical alfaisoform (mSMOa), have been reported recently [10,11]. The subcellular localization of the catalytically ... controlspeciﬁc primer pairs: b-actin-forward 5¢-TGTTACCAACTGGGACGACA-3¢ and b-actin-reverse 5¢-AAGGAAGGCTGGAAAAGAGC-3¢. Three separate experiments wereperformed from each RNA preparation.AcknowledgementsThis ... in NaCl ⁄ Pi(15 min at 4 °C) to evaluate the sub-cellular localization of the various isoforms. Determination of the subcellular localization of mSMOa,-l and -lD⁄V5-tagged proteins was carried...

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