Báo cáo khoa học: Contributions to catalysis and potential interactions of the three catalytic domains in a contiguous trimeric creatine kinase doc

... Contributions to catalysis and potential interactions of the three catalytic domains in a contiguous trimeric creatine kinase Gregg G. Hoffman1, Omar Davulcu2, Sona Sona1 and W. Ross ... residue(s) to selectively eliminate catalysis in each of the individual domains and in allpossible combinations of domains. Inactivation of thiscysteine has been shown to reduce catalytic turnover(kcat) ... two-domainarginine kinases [19–21], a two-domain carbonic anhy-drase [22], a three- domain luciferase [23] and a three- domain adenylate kinase [24]. The present studyprovides insight into the inter-dependent...

Báo cáo khoa học: Par j 1 and Par j 2, the two major allergens in Parietaria judaica, bind preferentially to monoacylated negative lipids potx

... Gonza´lez-Rioja R, Ferrer A, Arilla MC, Ibarrola I,Viguera AR, Andreu C, Martı´nez A & Asturias JA(2007) Diagnosis of Parietaria judaica pollen allergyusing natural and recombinant Par ... protein as the major protein in the surface wax of broccoli (Brassica oleracea) leaves. ArchBiochem Biophys 331, 460–468.48 Takishima K, Watanabe S, Yama da M, Suga T &Mamiya G (1988) Amino ... beencloned and sequenced, and their recombinant counter-parts were able to induce histamine release frombasophils of patients allergic to P. judaica pollen in a way comparable to that of the crude...

Báo cáo khoa học: Substrate speciﬁcity and transport mode of the proton-dependent amino acid transporter mPAT2 potx

... substrate spectrum thatincludes not only t he amino acids glycine, alanine, serine and proline but also osmolytes such as sarcosine and betaine, and the D-enantiomers of serine and alanine. The apparent ... the apparent Kmvalues of glycine and a lanine at pH values of pH 5.5–8.5 (Fig. 4) and a broad range of membranepotentials. At hyperpolarized membrane potentials Kmvalues of glycine and alanine ... polarity were analysed. The size of the amino acid side chain was shown to be a critical determinant for substrate interaction with PAT2.Only glycine and alanine were a ble to induce comparableinward...

Báo cáo khoa học: Dual mitochondrial localization and different roles of the reversible reaction of mammalian ferrochelatase ppt

... Shimokawa O, Matsui H & Taketani S(2005) Mechanisms involved in delta-aminolevulinicacid (ALA)-induced photosensitivity of tumor cells:relation of ferrochelatase and uptake of ALA to the accumulation ... trypsin. The amount of ferroch-elatase in the mitochondria was decreased by trypsintreatment, suggesting that a part of the ferrochelataseprotein is located at the surface of mitochondria. Alka-line ... ferrochelatase in mitochondria To examine the localization of ferrochelatase, the cDNA for ferrochelatase was transfected into Cos-7cells and the localization of expressed ferrochelatasewas compared...

Báo cáo khoa học: nNOS inhibition, antimicrobial and anticancer activity of the amphibian skin peptide, citropin 1.1 and synthetic modiﬁcations pot

... reactive radical NO [21,22]. Nitric oxide synthasesare composed of two domains: (a) the catalytic oxygenasedomain that binds heme, tetrahydrobiopterin and the substrate arginine, and (b) the ... supplying reductasedomain that binds NADPH, FAD and FMN. Communi-cation between the oxygenase and reductase domains isdetermined by the regulatory protein calmodulin whichinteracts at a specific ... is the meanstructure obtained by best-fitting and averaging the coordinates of backbone N, a- C and carbonyl-C atoms of the 20 final structures. (SA)ris the representative structure obtained after...

Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modiﬁed conotoxin, reveals that the glycan structure is a-D-Gal-(1ﬁ3)-a-D-GalNAc pot

... of N-acetylgalactosamine ( GalNAc) and galactose (Gal), but the interresidue linkage was notcharacterized. W e h ypothesized that t x 5a contained the T-antigen, b-D-Gal-(1ﬁ3) -a- D-GalNAc, ... on the t hreonine residue of tx 5a was previously investigated [9]. It was shown t hat the peptide contained N-acetylgalactosamine (GalNAc) and galactose ( Gal) in approximately e qual m olar amounts;however, ... b-D-Gal-(1ﬁ3) -a- D-GalNAc c ontaining glyco-peptide, and native t x 5a were simultaneously incubated a ndreacted with the same vial of the enzyme b-galactosidase.Native tx 5a, tx 5a hydrophilic, and tx5a...

Báo cáo khoa học: Site-directed mutagenesis and footprinting analysis of the interaction of the sunﬂower KNOX protein HAKN1 with DNA ppt

... USA). Ampliﬁcations were performed usingPfu DNA polymerase and oligonucleotides MALN1: 5¢-GCGGAATTCAAAAAGAGAAAGAAAGGG-3¢ and MALC:5¢-GGCCTGCAGCTAGAGAAGTGAAACATC-3¢ withHAKN1 cDNA [28] as the ... radicalfootprinting and interference assays A more detailed picture of the binding of HAKN1 to its target site was obtained by the analysis of footprint-ing patterns after cleavage of free and protein-boundDNA ... (5¢-TAAACCARAGGGTGCGGCAYTGGA-3¢), K54VR (5¢-TCCARTGCCGCACCCTYTGGTTTA-3¢), R55AF (5¢-CAAAGGAAGGCGCACTGGAA-3¢), R55AR (5¢-TTCCAGTGCGCCTTCCTTTG-3¢), R55KF (5¢-CAAAGGAAGAAGCACTGGAA-3¢) and...

Báo cáo khoa học: Phosphatidylserine induces functional and structural alterations of the membrane-associated pleckstrin homology domain of phospholipase C-d1 docx

... 187Phosphatidylserine induces functional and structuralalterations of the membrane-associated pleckstrinhomology domain of phospholipase C-d1Naoko Uekama, Takio Sugita, Masashi Okada, Hitoshi Yagisawa and ... diffraction. Asshown with dotted lines, the chemical shifts of the sig-nals of Ala116 and Ala118 are not affected by addition of PS, indicating that conformations of Ala116 and Ala118 within the ... C-terminal a3 -helix of the PHdomain are unchanged. These signals are indifferent to the presence of the membranes, probably because the positions of Ala116 and Ala118 are located far fromthe...

Báo cáo khoa học: Comparative metal binding and genomic analysis of the avian (chicken) and mammalian metallothionein potx

... (chicken) and mammalian metallothioneinLaura Villarreal1, Laura Tı´o2, Merce`Capdevila1 and Sı´lviaAtrian21 Departament de Quı´mica, Facultat de Cie`ncies, Universitat Auto`noma de ... involving a simultaneous intermolecularformation (N a1 –Cd a2 and N a2 –Cd a1 ) and intramolecu-lar loss (N a1 –Cd a1 and N a2 –Cd a2 ) of two Cd–NHisbonds, via the second nitrogen donor atom of ... BamHI and SalI (TakaraShuzo Co. Ltd, Kyoto, Japan) and subsequently ligatedinto the same sites of pGEX-4T-1. All the DNA constructswere conﬁrmed by automatic DNA sequencing (ABI 370,Perkin...

Báo cáo khoa học: Structure, mRNA expression and linkage mapping of the brain-type fatty acid-binding protein gene (fabp7 ) from zebraﬁsh (Danio rerio) potx

... 0.915 tcaCCAAttgaV$ECAT/NFY.01 nuclear factor Y )1203 (+) 1.000 0.937 aggacCCAAtaagggaV$GATA/GATA2.02 GATA-binding factor 2 )177 (–) 1.000 0.912 agcGATAttaV$GATA/GATA1.03 GATA-binding factor 1 ... taaaGATAaacaaV$GATA/GATA1.02 GATA-binding factor 1 )940 (+) 1.000 0.965 taagaGATAatcggV$SORY/SOX5.01 Sox-5 )200 (–) 1.000 0.860 attaCAATtgV$SORY/SOX5.01 Sox-5 )561 (–) 1.000 0.989 caaaCAATgcV$SORY/SOX5.01 ... 5¢-GTACATATTGTCGTTAGAACGCGTAATACGACTCACTATAGGGA-3¢,3¢-CATGTATAACAGCAATCTTGCGCATTATGCTGAGTGATATCCCTCTAG-5¢,usingT4DNA ligase (Promega). Following precipitation, the DNAwas resuspended in 15 lL of sterile,...

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