Báo cáo khoa học: Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures potx

... 19REVIEW ARTICLE Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures Alexander Wlodawer1, Wladek Minor2,3, Zbigniew ... or processing error in the PDB.Another resolution-related problem is whether indi-vidual B-factors were reﬁned, or whether only anoverall B (for the whole structure) or group B-factors (for ... is therefore another important parameter ofdata quality. The above numerical criteria are usually quoted for all data and for the highest resolution shell. Unfortu-nately, it is not customary...

Tài liệu Báo cáo khoa học: "ENGLISH WORDS AND DATA BASES: HOW TO BRIDGE THE GAP" pdf

... with the formal query which would formulate in terms of the actual data base structure what the desired information is. An important decision in the design of a q.a. system is therefore, how to ... This may seem to force us to give up local translation for certain cases: instead, we would have to design an algorithm which looks out for sub-expressions of the form I ignore the complexities ... performed. There- fore, the result of the EFL -to- DBL translation is processed by a module which applies logical equi- valence transformations in order ~o simplify the expression. At the...

Báo cáo khoa học: "A System for Large-Scale Acquisition of Verbal, Nominal and Adjectival Subcategorization Frames from Corpora" pot

... than the ones reported here for the newsystem.5 The results for different word classes are not directly com-parable because they are affected by the total number of SCFsevaluated for each word ... with the size of the inputdata to acquisition (the more data, the better perfor-mance). When considering the F-measure results for the individual words shown in Table 3 there appears to be ... and testdata for subcategorization acquisition. The tool in-corporates an intuitive interface with the ability to signiﬁcantly reduce the number of frames presented to the user for each sentence.We...

Báo cáo khoa học: "Ranking Algorithms for Named–Entity Extraction: Boosting and the Voted Perceptron" pdf

... .InitializeSetSet For all , set .Set For , calculate–––Repeat for = 1 to ChooseSetUpdate one parameter, for ––– for ,– for ,– for ––– for ,– for ,– For all features whose values ofand /or have ... features of the words before/after the startof the entity (16 features total, only 4 shown) TO0 00= TO1 11= TO2 000= TO2 111=Trigram boundary features of the words before/after the endof the entity ... will use vec-tor notation. Deﬁne to be the vector. Then the ranking scorecan also be written as whereis the dot product between vectors and .4.2 The boosting algorithm The ﬁrst algorithm we...

Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf

... 50 lMSB-415286 for 60 min prior to incubation withinsulin (1 nM or 100 nM) for 10 min (for phospho-blots) or for 30 min(uptake assays). Cells were lysed and 50 lglysateproteinwasimmunoblotted ... pretreated for 5 min with 100 nMwortmannin, 10 lMSB-203580, 10 lMPD-98059 or 10 lMrapamycin prior to cell stimulationwith 100 nMinsulin for 30 min or 50 mMLi for 60 min. Inhibitors were ... < 0.01). (B) For GS activity, cells were treated with 50 mMLi or 10 lMSB-203580 for 60 min or with 100 nMinsulin for 10 min prior to assaying incorporation of glucose from uridine diphospho-[6-3H]-D-glucose...

Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt

... afﬁnities for sugars but of whether the protein adopts the openform or the closed form. TvuCMBP–c-CD complex isseen as the closed form (Fig. 2A,B), and this struc-ture shows that the sugar-binding ... cleft formed by the twodomains. A common functional conformational change has been reportedin this protein family, which involves switching from an open form to a sugar-transporter bindable form, ... does not have the ability to perform the proper interaction with the MalFGK2sugar-transporter complex. Interestingly, EcoMBPadopts the open form in the unliganded protein butalso in the complex...

Báo cáo khoa học: Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate potx

... scaffold proteins interact withp38 MAPK isoforms, how these interactions are regu-lated, how they interplay with catalysis, how they maybe exploited therapeutically or how they differ within the ... the inhibitor displays selectivity towards certainenzyme forms. We believe the uncompetitive patterntowards ATF2D115 (no slope effect) results becauseMgADP does not bind the free form of the ... GST fusion protein in E. coli BL21 (DE3) pLysS. The protein was puri-ﬁed by GSH-afﬁnity chromatography according to stand-ard protocols (Sigma Product Information for G4510).Pooled protein fractions...

Báo cáo khoa học: "Medical treatment for the terminally ill: the ‘risk of unacceptable badness’"

... loved one. Once life-supporting care is instituted, the patient has options for ‘survival’ that they did not have before, even though theyare dependent on ‘life support’. There are now variablesCommentaryMedical ... theirauthority to make choices extends to making bad ones.2. Moribund patients look comfortable on ‘life support’. Anobserver’s primal reaction to the vibrant external appearanceof a body supported ... byinstituting the most invasive life support technology. However,if organ system failure is not reversible, then the reasoningbehind life support technology becomes moot. We must thenbe prepared to remove...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... A˚2 for main-chain atoms, 35.7 A˚2 for side-chainatoms and 33.8 A˚2overall (3244 atoms). For the pep-tide, B factors are slightly higher than those for importin-a: 36.9 A˚2 for main-chain ... according to the following equation:Bz¼BiÀ ljrjwhere Bzis the normalized B factor, Biis the B factor ofatom i, ljis the mean B factor (excluding water moleculesand peptide atoms) ... help-ing to facilitate the release of the cargo within the nucleus [10,11]. The removal of the autoinhibitorydomain to create a truncated importin-a avoids possi-ble competition for the binding...

Tài liệu Báo cáo khoa học: A strategy for discovery of cancer glyco-biomarkers in serum using newly developed technologies for glycoproteomics ppt

... were converted to their corre-sponding alditols before MS acquisition. The othertypes of glycan stored in the GMDB are mostly taggedwith 2-aminopyridine, which can be used for fluo-rescence ... on the principle of evanescent-field fluorescencedetection (Fig. 3A). Furthermore, they succeeded inimproving the array platform analysis to achieve the highest sensitivity reported to date (the ... approach to identify cancer glyco-biomarkers[35,36]. The authors recovered all of the glycoproteins from serum, released the N-glycans from the totalglycoprotein pool by PNGase digestion, and thenperformed...

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