Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identification of amino acid residues critical for eIF5A activity and hypusine modification doc
Báo cáo khoa học: Mutational analyses of human eIF5A-1 – identification of amino acid residues critical for eIF5A activity and hypusine modification doc
... claim to original US government works Mutational analyses of human eIF5A- 1 – identification of amino acid residues critical for eIF5A activity and hypusine modification Veridiana S. P. Cano1,2,*, ... struc-tures of both the N-terminal and C-terminal domains of eIF5A (Fig. 1, amino acids 1 7–8 2 and 8 5–1 46) arenecessary for the biological functions of eIF5A. The loss of eIF5A function for various ... there is noinformation available as to which parts of the eIF5A molecule and the ribosome are involved in the inter-action.The identification of amino acid residues critical for eIF5A activity is...
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Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot
... 1. 1–1 .8 1. 1–1 .8IRS1 0. 6–0 .7 0. 6–0 .7 1. 8–2 .0 1. 8–2 .0 1. 8–2 .0 1. 8–2 .0PKB 0. 9–1 .1 0. 3–0 .4 0. 6–0 .7 0. 6–0 .7 0. 6–0 .7 0. 6–0 .7Glucose transport 0. 1–0 .2 0.0 2–0 .03 0. 1–0 .2 0. 1–0 .2 0. 1–0 .2 0. 1–0 .2A. Danielsson ... (MAP) kinases – extracellularsignal-related kinase (ERK) 1 and 2 [3], and p38 [4,5] – protein kinases that phosphorylate and control the activity of other downstream protein kinases and Keywordsglucose ... (C).Fig. 4. Dose–response effect of insulin on phosphorylation of insu-lin receptor, IRS1, and PKB before (s) and after (d) overnightrecovery. Whole cell lysates, of adipocytes form control subjects,were...
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Báo cáo khóa học: Binding analyses between Human PPARc–LBD and ligands Surface plasmon resonance biosensor assay correlating with circular dichroic spectroscopy determination and molecular docking ppt
... products for discovering active compounds and targetinformation. Curr. Medical Chem. 10, 232 7–2 342.Ó FEBS 2003 Binding analyses of human PPARc–LBD to ligands (Eur. J. Biochem. 271) 397Binding analyses ... energies of the ligands binding to PPARc. The binding free energies (kcalÆmol)1) of the protein–ligand complex wereestimated by the scoring function of AUTODOCK3.0.Number Ligand – log (KD) ... cis-Parinaric acid is a ligand for the human peroxisome proliferator activated receptor c:development of a novel spectrophotometric assay for the discovery of PPARc ligands. FEBS Lett. 431, 47 6–4 80.21....
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... cleft. The arginase loop (residues 12 6–1 43 inthe sequence of human liver arginase) contains Asn130 and other residues proposed as ligands for the a-carb-oxylate group of the substrate arginine. ... shown in Table 1, upon mutation of Asn130 toaspartate, the arginase activity of human arginase I wasreduced to about 17% of the wild-type activity, and theKmvalue for arginine was increased about ... 2006)doi:10.1111/j.1742-4658.2006.05551.xUpon mutation of Asn130 to aspartate, the catalytic activity of human arginase I was reduced to 17% of wild-type activity, the Kmvalue for arginine was increased 9-fold, and the kcat⁄...
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Tài liệu Báo cáo khoa học: Mutational analysis of plasminogen activator inhibitor-1 Interactions of a-helix F and its neighbouring structural elements regulates the activity and the rate of latency transition pdf
... 172Consensus – G K I – DELLV – – VLID––TPAI-1 K G M I S N L L G K G A V D Q L Ta1PI QGKI VDLVK– – ELDRDTATIII E G R I T D V I P S E A I N E L TÓ FEBS 2003 Mutational analysis of PAI-1 ... substitution of the residues F100, V126, F128 and I137, respectively, led to increased substrate beha-viour and a low unstable inhibitory activity, and for I137A a biphasic loss of activity (Fig. 2 and ... in green, a-helix F inorange and parts of b-sheet A in pink. Backbone atoms of the s1A/hF-loop (residues D127 to E130) and of the top of hF and the hF/s3A-loop (residues T146 to L154) are shown...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... H. (1993) Identification of glutamic acid 204 and aspartic acid 200 in chitinase A1 of Bacillus circulans WL-12 as essential residues for chitinase activity. J. Biol. Chem. 268,1856 7–1 8572.27. ... being formed, and covalent bonds being formed and broken). Thus, the calculated effect of rotation of Asp142 on the pKa of Glu144 only gives an indication of what may happen to the acidity of Glu144. ... of subsets of these conserved residues in various family 18 chitinases hasshown for the majority that they are important for catalysis[20,2 6–3 1]. However, the mechanistic roles of several of these...
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Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf
... 45Nicotinonitrilekcat(s)1) 131 – – – – Km(mM) 0.12 – – – – kcat/Km(s)1ÆmM)1) 1090 – – – – Isonicotinonitrilekcat(s)1)9 0– – Km(mM) 0.079 – – – – kcat/Km(s)1ÆmM)1) 1140 – – – – Cobalt contentCo/ab ... various inhibitor concen-trations ( 0–1 0 mM for n-butyric acid, 0–5 0 mM for propionic acid, and 0–1 00 lM for benzoic acid) and two substrate concentrations (0.5 and 5 mMmetacrylo-nitrile).Site-directed ... reduced stability and enzymatic activity [17,18]. Of the residues of P. thermophila NHase participating inthe recognition of a substrate, three (bLeu48, bPhe51 and bTrp72) form a hydrophobic...
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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx
... [19], and NMRspectroscopic data of p25a homologs from mice,Caenorhabditis elegans and humans [2 0–2 2] demon-strate a folded central core and unfolded N- and C-ter-minal extensions of 45 and ... [28]. The instability of DA at the neutral pH of the cytosol causes the forma-tion of toxic aminochromes, and these species are notformed when DA is protonated at the acidic pH of thestorage vesicles. ... 45 and 70 amino acid residues, respectively. We constructed two deletion mutants:p25aDN, which lacked N-terminal residues 3–4 3, and p25aDC, which lacked C-terminal residues 15 6–2 19(Fig. 2A)....
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt
... Positions of E665 and E555 of the CBP–KIX domain, and residues K291 and R294 of the c-Myb transactivation domain are indicated. (C) TheCBP–KIX:cMyb:MLL1 TAD ternary complex (drawn form PDB code: ... TAD is shown in green and the colors for the CBP–KIX:cMyb are as in (B). Upon formation of the ternary complex, residues E665 and E666 of the CBP–KIX domain become ordered and interact with the ... chan-nel that divides a pair of acidic lobes, one of which iscomposed of residues from the SET-I region and theother of residues from the SET-C and post-SETregions. Lysine 4 of histone H3 is inserted...
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