Báo cáo khoa học: Role of active-site residues of dispersin

Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

... chitinases are retaining glycoside hydrolases that have been found in many organisms varying from bacteria to humans [1,2]. The catalytic domains of family 18 chitinases have a (ba) 8 (TIM barrel) ... Asp140, whichappearstobecrucialinChiBandinchitinaseA1 from Bacillus circulans, whereas it may be mutated to asparagine without loss of activity in other family 18 chitinases [28,30...

Ngày tải lên : 07/03/2014, 14:20

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Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf

... 5¢-GCCACT TACTTGAACTTTGACATAGAAGCCGG-3¢,5¢-GCCAC TTACTTGGCATTTGACATAGAAGCC-3¢,5¢-GCCACT TACTTGGACTTTAACATAGAAGCCGG-3¢,5¢-GCCAC TTACTTGGACTTTGCGATAGAAGCCGG-3¢,5¢-GGAC TTTGACATACAAGCCGGTATCGATGC-3¢,5¢-GGACT TTGACATAGCGGCCGGTATCGATGC-3¢ and 5¢-GGA TCACTAGCCTTCGCGAGTGTAGACAGAG-3¢, ... 670–680. 29 Mine S, Nakamura T, Hirata K, Ishikawa K, Hagihara Y & Uegaki K (2006) Crystallization and X-ray...

Ngày tải lên : 15/03/2014, 11:20

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Báo cáo khoa học: Role of active-site residues of dispersin B, a bioﬁlm-releasing b-hexosaminidase from a periodontal pathogen, in substrate hydrolysis pptx

... Forward CCACAGAATAACCAAA TTGATCGCCACC Reverse Y18 7A GATGAATTTGGT GCGTCTGTGGAAAG Forward CTTTCCACAGA CGCACCAAATTCATC Reverse W23 7A CCGAATATTGAAATTACTTAT GCGAGCTATGATGGCG Forward CGCCATCATAGCT CGCATAAGTAATTTCAATATTCGG ... 5999 Role of active-site residues of dispersin B, a bioﬁlm-releasing b-hexosaminidase from a periodontal pathogen, in substrate hydrolysis...

Ngày tải lên : 16/03/2014, 06:20

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Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... ﬁndings suggest that ablation of KCs protects against hepatic drug-metabolizing dysfunction by modulation of the in ammatory response. Abbreviations ALT, alanine aminotrasferase; AST, aspartate ... expression and activity of CYP2E1 were downregulated in a rat hepatoma cell line after the administration of proinﬂammatory cytokines, leading to a loss of catalytic activity. This...

Ngày tải lên : 14/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... several adhesins such as BabA, BabB, SabA, AlpA and AlpB, as well as OipA, which mediate apical binding of the bacteria (1). Attached H. pylori or those in the mucus secrete virulence factors into ... restricted areas before a limited number of bacteria gain access to integrins and inject CagA. The basal injection model of CagA can also explain why H. pylori does not cause more gast...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... 5¢-GTAGGCATGAGAACGGGA AG-3 and for reverse 5¢-GGGGGTAAGAGGAGGAGA AA-3¢ and for CERK-negative forward 5¢-CCGCAAG AGGCTTTATTGTC-3 and reverse 5¢-TATGCCAAGGA CACGGAGAT-3¢, as a negative control ... Biotechnology (Lake Placid, NY, USA). A MEBCYTO Apoptosis Kit was purchased from Medical and Biological Laboratories (Nagoya, Japan), and a Nuclear Extract Kit was from Active Motif (Carlsba...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... determination, enzyme assays and materials CT -A and CT -B, native CT, acridine orange and pepsta- tin A were purchased from Sigma (St Louis, MO, USA). A nontoxic diphtheria toxin CRM 197 mutant and baﬁlomy- cin -A1 ... obtained from Calbiochem (San Diego, CA, USA). Rabbit polyclonal anti-(CT C3062) was obtained from Sigma. Rabbit polyclonal IgG directed against Gsa proteins was o...

Ngày tải lên : 19/02/2014, 02:20

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Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... intracellular signaling pathways [17,18]. Activation of tyrosine kinase receptors is involved in cell survival through downstream signaling cascades such as the MAP kinase and phosphatidylinositol-3¢-OH ... growth factor transmitted protective signals against ADR-induced apoptosis by causing activation of the phosphatidylinositol-3¢ -OH kinase (PtdIns3-K) ⁄ Akt signaling pathway in...

Ngày tải lên : 19/02/2014, 06:20

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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by ﬂuorescence resonance energy transfer and the role of ATP hydrolysis pptx

... strand exchange assay were synthesized by Metabion (Martinsreid, Germany) with the following sequences: PhiC: 5¢-CGATACGCTCAAAGTCA AAATAATCAGCGTGACATTCAGAAGGGTAATAAG AACG-3¢;, PhiW: 5¢-CGTTCTTATTACCCTTCTGAA TGTCACGCTGATTATTTTGACTTTGAGCGTATCG-3¢ and ... 5¢-CGTTCTTATTACCCTTCTGAA TGTCACGCTGATTATTTTGACTTTGAGCGTATCG-3¢ and M13C: 5¢-CTACAACGCCTGTAGCATTCCACAGA CAGCCCTCATAGTTAGCGTAACGAGATCG-3¢. Phi-C...

Ngày tải lên : 19/02/2014, 07:20

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Tài liệu Báo cáo khoa học: Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus doc

... with a modiﬁcation in the mathematical processing of the raw data, as previously described [48]. The measurements were performed at 25 °C in NaCl ⁄ P i containing 1% BSA. Fab4E11-H6 at a constant ... kinetic data were analysed with the biaevaluation 3.0 software (Biacore). The active concentration of each Fab4E11-H6 preparation was determined as described [50]. Brieﬂy, 500 RU of...

Ngày tải lên : 19/02/2014, 07:20

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