Báo cáo khoa học: Kinetic modeling can describe in vivo glycolysis in Entamoeba histolytica doc
Báo cáo khoa học: Kinetic modeling can describe in vivo glycolysis in Entamoeba histolytica doc
... (2007) 4922–4940 ª 2007 The Authors Journal compilation ª 2007 FEBS Kinetic modeling can describe in vivo glycolysis in Entamoeba histolytica Emma Saavedra1, Alvaro Marı´n-Herna´ndez1, Rusely ... experi-mentally determined. In the kinetic model described in the present work, the in uence of using Keq in the rateequations was eliminated by introducing the actual Vm00 20 40 60 80 10020406080100PPDKPGAMPPi-PFKHKHK ... Metronidazole resistance in the protozoanparasite Entamoeba histolytica is associated withincreased expression of iron-containing superoxideE. Saavedra et al. Modeling Entamoeba glycolysis FEBS Journal...
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Báo cáo khoa học: "Improved Modeling of Out-Of-Vocabulary Words Using Morphological Classes" docx
... on unknown histories.1 IntroductionOne of the challenges in statistical language mod-eling are words that appear in the recognition taskat hand, but not in the training set, so called out-of-vocabulary ... classes to handling OOVwords in language modeling for English. Previouswork on morphological classes in English has notbeen able to show noticeable improvements in per-plexity. In this article ... modelthat can naturally integrate unknown words. In related work, factored language models(Bilmes and Kirchhoff, 2003) were proposed tomake use of morphological information in highly in ecting languages...
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Báo cáo khoa học: Determining and understanding the control of glycolysis in fast-growth tumor cells Flux control by an over-expressed but strongly product-inhibited hexokinase pdf
... forwardreaction. In this regard, it is well documented thatGlc6P is a potent inhibitor of hexokinase-I, hexo-kinase-II and hexokinase-III [37]. In consequence, theincreased hexokinase activity ... original intracellular concentration.Determination of glycolytic flux in liver andtumor cellsCells (15 mg proteinÆmL)1) were incubated in 3 mL Krebs–Ringer medium with orbital stirring (150 ... reported thathexokinase-II plays an important role in preventingapoptotic events, such as cytochrome c release in HeLacells, by interfering with the binding of the pro-apop-totic protein Bax to the...
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Tài liệu Báo cáo khoa học: Kinetic analysis of effector modulation of butyrylcholinesterase-catalysed hydrolysis of acetanilides and homologous esters pdf
... site of serotonin cannot be inferredfrom these results. Although serotonin can bind to theactive site binding locus (W82), binding to PAS cannotbe ruled out. If serotonin binds only to W82, ... from tyramine binding toPAS. Such qualitatively opposite effects can be tenta-tively interpreted in terms of allosteric inhibition ⁄ acti-vation: the binding of tyramine to PAS induces aconformational ... by tyramine). Kinetic constants were determined by: (a) linearizing theDixon ⁄ Cornish–Bowden plots [74], followed by fitting theline with Sigma Plot 4; (b) re-plotting the slopes fromLineweaver–Burk...
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... 3¢splice site can play a role in determining the binding regis-ter of reaction substrates. Biochemistry 44, 1067–1077.P. P. Dotson II et al. Kinetics of the trans excision-splicing reactionFEBS ... reaction is4.1Æmin)1(Fig. 2), with a typical end point of 70–80%.Over the period of 15–60 min, this end point does notchange, indicating that either an internal equilibriumTable 1. Kinetic parameters ... Self-splicing of group I introns. AnnuRev Biochem 59, 543–568.58 Been MD & Perrotta AT (1991) Group I intron self-splicing with adenosine: evidence for a single nucleo-side-binding site....
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... self-standingstructure protruding from the interface between theDNA binding and the active site subdomains, we man-aged to model the entire domain without it.Another significant finding concerns ... 51–55.Supporting informationThe following supporting information is available:Fig. S1. The in uence of (A) divalent cations, (B) pHand (C) temperature on Rep245 polymerase activity.This supporting information ... used. In addition, a longer variant comprising the N-ter-minal residues 1–516 (Rep516) and lacking the C-ter-minal ATP⁄ GTP-binding site motif A was alsodesigned and the truncated protein was...
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Tài liệu Báo cáo khoa học: Kinetic basis for linking the first two enzymes of chlorophyll biosynthesis doc
... were preincubated in the pres-ence (s) and absence (d) of 0.75 lM ChlH in syringe 1. Syringe 2contained only MgD. (A) MgDME evolution was followed by integ-rating the peaks at 12.3 min on the ... ofthis reaction intermediate, resulting in a reduction in the lag phase of product accumulation, and an increase in initial rate of product evolution. Furthermore, thepresence of ChlH increases the ... pre-equilibrated with three columnvolumes of binding buffer. The column was washed with10 column volumes of binding buffer and 6 column vol-umes of binding buffer containing 60 mm imidazole (washbuffer)...
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Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx
... minor, implying rotations of domains ofjust a few degrees (e.g. 7° interdomain rotations upontRNA binding to the Tt-GluRS and local limitedrearrangements in the active site) [5]. Interestingly, ... provide a rationale for thediscriminating behaviour of Mt-GluRS in E. coli.Studies on Tt-GluRS indicated that discriminatingand nondiscriminating GluRS can be distinguished onthe basis of the ... ethanol, with interval stirring (10 min each) followed.Dried filters were placed in an 8 mL plastic vial. Radioactiv-ity was determined by scintillation counting in a TriCarb2100-TR (Perkin–Elmer,...
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Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt
... designed against methionine gamma-lyase-containing pathogens, has efficacy in vitro and in vivo against Trichomonas vaginalis. Antimicrob AgentsChemother 45, 1743–1745.27 Inoue H, Inagaki K, ... but were minor contaminants in the recombinant protein preparations. To examinewhether irrelevant proteins can also serve as scavengersof carbonothionic difluoride produced from TFM byKinetics ... involved in the degradation ofsulfur-containing amino acids (SAAs), have beenexploited as a target for chemotherapeutic intervention in cases of cancer and infectious diseases [2,3]. Methio-nine...
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Báo cáo khoa học: Kinetic properties of catecholoxidase activity of tarantula hemocyanin pot
... Canters G (2000) Kinetic and paramagneticNMR investigations of the inhibition of Streptomycesantibioticus tyrosinase. J Mol Catal 8B, 27–35.38 Salvato B & Beltramini M (1990) Hemocyanins: ... in l:g2-g2coordination at type 3 copper centers [5,6].Type 3 copper centers cannot only reversibly bindoxygen, as in hemocyanins, but can also activate andmetabolize oxygen in enzymes such as tyrosinase ... of enzyme kinetics, the conditions with constantactivity were established. This was accomplished bypreincubating hemocyanin with 5 mm SDS for 5 minbefore adding the substrate. Using this condition,which...
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Từ khóa: Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDETrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtBÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ