... HepG
2
cDNA library (C. Baisez
and A. Harduin-Lepers, unpublished data), as the template
and two specific primers For 6I 5¢-CGATGAATTC
GTTAACGCTCATCACCATCACCATCACGGGAAA
TTGGCCATGGGGT-3¢ containing a HpaIsiteandBack
6I ... of glycoprotein oligosaccharides N-linked to asparagine.
Enzymatic characterization of a Galb1–3 (4) GlcNAc a2 ,3-sial-
yltransferase and a Galb1–4GlcNAc...
... 275–283.
10 Kuwada M, Teramoto T, Kumagaye KY, Nakajima K,
Watanabe T, Kawai T, Kawakami Y, Niidome T,
Sawada K, Nishizawa Y et al. (1994) Omega-agatoxin-
TK containing D-serine at position 46, but ... crosspeaks were assigned and inte-
grated, with concomitant cycles of structure calcula-
tions for evaluation of distance and angle constraint
violations as well as assignments of add...
... grateful to Dr Mariagrazia Squillace for excel-
lent technical assistance and helpful discussion and to
Dr Gabriella Fiorentino, Dr Pietro Amodeo and Dr
Francesca Maria Pisani for critical reading ... shortest functional domain from a crenarchaeal plasmid endowed with
DNA and RNA synthesis and terminal transferase activity.
Abbreviations
AEP, archaeo-eukaryotic replicative prima...
... GAGAATTCAAAAGAAAGCGGGGAAGAA Construction of pOpacWh
W12 CGAGATCTTGTGGACATGGTCCCGTTTC Construction of pOpacWh
S1 CGGAATTCGCTAATGGGAGGAAATCAC Construction of pOpacSh
S2 TACAGATCTTTTCGTCTTGCGAATTTC ... of sipW
W7 TTGTGTAAAAGTGATGACATCGCC Cloning of sipW
W8 GTGATCCCGATTATTCTGTGTGTT Cloning of sipW
W9 GGCGATGTCATCACTTTTACACAA Cloning of sipW
W10 AACACACAGAATAATCGGGATCAC Cloning of...