Báo cáo khoa học: Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice doc

... clearance of soluble CD69 receptors in the blood- stream of C57BL ⁄ 6 mice. (A) The 125I-radiolabeled recombinant pro-teins were injected into the tail vein of the mice and the radioactivity in individual ... 2008 The Authors Journal compilation ª 2008 FEBS Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact ... both the long circulation and the intactness of the moststable human CD69 protein (together with its rat and mouse orthologs) in the blood of mice. For theseproteins, approximately 40% of the initial...

Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane afﬁnity and hyper-anticoagulant activity in mouse plasma pptx

... concentration in the membrane results in an increase in the number of binding sites (compare Fig. 3D and 3E) and also in an average site with increased afﬁnity. If Gla proteinswere to bind exclusively to ... mem-brane binding afﬁnity enhancement by minor increases in the overall on-rate and ⁄ or minor decreases in the overall off-rate. The results obtained in the present study supportthat the binding ... shown to enhance the assembly and function of several clot-ting factor complexes [22,23]. We also show that POPE in uences the binding of mouse protein C. Comparing the binding data of 20-10-70 and...

Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx

... available:Fig. S1. Amino acid alignment of linker region and ligand-binding domain (LBD) of ecdysone receptors. Fig. S2. Amino acid alignment of linker region and ligand-binding domain (LBD) of ultraspiracle ... images. The pixel intensity of the bandsignal was determined for the deﬁned band area and adjustedrelative to one of the signals, as designated, to calculate the relative band density.Vector description ... agonists in the cell culture system involve not only allosteric changes in the receptor itself, but also factors such as the effect of DNA and ligand on receptor stability and the regulation of nuclear...

Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx

... clone and gave the remaining part of the coding region. The coding part of the Y6gene is contained within one exon and encodes a protein of 374 amino acids displaying the characteristics of other ... severalother primates [32,34,36], pig [37] and guinea-pig [38], and it has been lost in rat [39]. On the other hand, the gene has an intact open reading frame in a distantrelative of the pig, the ... compared to the GenBank databaseusing the On-Line blastx program and found to be sim-ilar to the mammalian Y6 receptors. The cloned insert waslabeled using the Random Primer Labeling Kit (Amer-sham...

Báo cáo khoa học: Human recombinant prolidase from eukaryotic and prokaryotic sources Expression, puriﬁcation, characterization and long-term stability studies pptx

... vector pET16b containing an N-terminal His tag and the sequencefor the Factor Xa cleavage site in- frame with the human prolidase sequence under the control of the T7 promoter.Human recombinant ... 55% of the initial activity and the untagged prolidase 66% (Fig. 5B). The addition of MnCl2 and GSH showed the mostpromising effects in stabilizing and activating the enzyme. Both tagged and ... different substrates, in the presence of various bivalent ions, in the presence of the strong inhib-itor Cbz-Pro, and at different temperatures and pHs. The recombinant enzyme with and without a tag...

Báo cáo khoa học: "Veterinary decision making in relation to metritis - a qualitative approach to understand the background for variation and bias in veterinary medical records"

... understanding with regard to decision levelsBased on analysis of the veterinarians' perceptions of howthey wished to use the metritis score in their practice and on dialogue with the farmer and ... scores and veterinarians' decision making connected to metritis treat-ment and potential links to data quality. This understand-ing provides insight into potential errors (bias and random ... and the inter-viewed during the interviews that could influence the statements of the interviewed e.g., the use of leading ques-tions. In the phase of analysis it is important to determine...

Báo cáo khoa học: "Medical emergency teams: deciphering clues to crises in hospitals"

... and the incidence of crisis recognition in hospitals. Their review of over 2000 events revealed an increase in events at certaintimes of the day, notably near nursing handoffs and physicianrounding. ... patients who are in crisis, and then respond to them swiftly and effectively to prevent unnecessary deaths. In 1994, Franklin and Mathew [1] recognized that cardiacarrests in hospitals are often preceded ... teams and METs. In North America and in Europe, there now appears to be a rapid increase in number of organizations that have implemented a MET program, following a trend set in Australia. The...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... ligands: the ﬁve same ligands in the Mn-reconstituted enzyme and an additional water oxy-gen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed an equatorial ... had an independent metal-binding siteat the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the ... residuesinvolved in the binding of the metal cofactor.ApeSODs in different forms were superimposed with the least square ﬁt of Ca atoms of the residues in the range10–200. ApeSOD and P. shermanii...

Tài liệu Báo cáo khoa học: The plasminogen activator inhibitor 2 transcript is destabilized via a multi-component 3¢ UTR localized adenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenes pdf

... SJS259 and SJS260, and the creation of the ARE II ⁄ ARE IV double mutant used primers SJS261 and SJS262. The mutagenesis of ARE I and III introducedHindIII restriction sites and so the creation of ... was analysed byRNase protection assay or by northern analysis (10 lgoftotal RNA) using the intron spanning32P-labelled globin and GAPDH probes described above. The b-globin and GAPDH mRNA band ... activator inhibitor 2 transcript isdestabilized via a multi-component 3¢ UTR localizedadenylate and uridylate-rich instability element in an analogous manner to cytokines and oncogenesStan Stasinopoulos1,...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... binding. Pointmutations disrupting the RNA-binding capacity of any of the two RRMs led to the nuclear sequestration of the reporter protein, conﬁrming the inability of suchmutants to exit the ... in the presence of RNAse. Interestingly, most of the identi-ﬁed proteins are known to be involved in RNA metab-olism and belong to three large families of RNA-binding proteins. These proteins ... factors(RNA-binding proteins and noncoding regulatoryRNAs) that bind to these sequences. Among the beststudied regulatory sequences, the adenine and uridine-rich elements (AREs) located in the 3¢ UTR of mRNAs...

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