... tem-peratures.Strategies for enhancing thermalstability and activity ofhyperthermozymesCrucial for the development and optimization of high-temperature biocatalysis systems is the need to gainfurther understanding ... the stem, and withtheir Topt[15].In spite of attempts to correlate the G-C content ofhyperthermophilic genomes with their Topt, it shouldbe noted that experiments performed in vitro and ... synthases from the hyper-thermophilic Pyrococcus furiosus ( Topt¼ 100 °C), the thermophilic Thermoplasma acidophilum (Topt¼55 °C), the mesophilic mammal (pig; Topt¼ 37 °C), and the psychrophilic...