Báo cáo khoa học: Structural features and dynamics of a cold

Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx

... reduce catalytic activity of a cold- adapted alkaline phosphatase. Biochim Biophys Acta 1774, 679–687. 32 Janeway CML, Xu X, Murphy JE, Chaidaroglou A & Kantrowitz ER (1993) Magnesium in the active ... stability of Vibrio alkaline phosphatase, gave a coinci- dental reduction in mobility of a nearby spin-label located at C67, as determined by EPR spectroscopy. Th...

Ngày tải lên : 16/03/2014, 01:20

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Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx

... kinetic parameters K m , V max and k cat were determined for the puriﬁed AATB3. Values for K m and V max for both amino donors (l-aspartate and l-glutamate) and ac- ceptors (a- ketoglutarate and oxaloacetate) ... mML-aspartate was used as amino donor for a- ketoglutarate, and 30 m ML-glutamate was used as amino donor for oxaloacetate. The activity of a- ketoglutarate was adj...

Ngày tải lên : 14/03/2014, 23:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii Maghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S. Jeya Suriya Department ... animal glycoconjugate. They act as an important component of the ligands recognized by the lectins. Recognition can be affected by specific structural variations...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... of AF499 was identiﬁed as an archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a high le vel of identity with th e consensus se quence ()35 to )23, AAANNN TTATATA) ... with an apparent molecular mass of 53 kDa appears as a double band in unboiled samples (lanes A1 and B1). Table 1. N-Terminal sequences of the polypeptides of the puriﬁed enzy...

Ngày tải lên : 21/02/2014, 03:20

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Báo cáo khoa học: Identiﬁcation and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... bp was produced by PCR ampliﬁcation with TOPO 2.1-SmNR1 as a template (forward primer: 5¢-ATTTCAGAAGTTGAAC AAACACAC-3¢, reverse primer: 5¢-AAGATGGTATT GAAGATGATGGTTGA-3¢), puriﬁed from agarose ... DR2: 5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCG TAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAA GGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGG TCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCAAGGT CATGACCTCG-3¢. One strand...

Ngày tải lên : 07/03/2014, 11:20

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Báo cáo khoa học: Puriﬁcation and properties of a new S-adenosyl-Lmethionine:ﬂavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot

... compo- nents of each reaction mixture, after their chromatographic separation and puriﬁcation, were submitted to 1 HNMR (nuclear magnetic resonance) and FABMS (fast atom bombardment mass spectrum) analyses ... The concentration of both the assayed compound and its respective transformation product was determined by comparison of peak data with those obtained from authentic stand...

Ngày tải lên : 08/03/2014, 08:20

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Báo cáo khoa học: Proper targeting and activity of a nonfunctioning thyroid-stimulating hormone receptor (TSHr) combining an inactivating and activating TSHr mutation in one receptor pptx

... Dickinson and Co.) was used to acquire and analyze data. A minimum of at least 10 000 cells was analyzed. Computation of speciﬁc constitutive activity (SCA) and relative SCA (RSCA) Given that the transfection ... directed against the extracel- lular domain of the TSH receptor (NCL-TSH-R2, Novo- castra Laboratories Ltd, Newcastle, UK) (data not shown), and by FACS analysis us...

Ngày tải lên : 08/03/2014, 08:20

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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx

... oyster CaM and CaLP. In the presence of calcium, oyster CaLP and CaM appeared as a single band with an apparent molecular weight of approximately 18 kDa and 14 kDa, respectively, whereas in the absence ... 5¢-untranslated sequence, an open reading frame consisting of 483 bp, a TGA stop, a 146 bp 3¢-untranslated sequence, and a poly (A) tail of 18 nucleotides. A putat...

Ngày tải lên : 16/03/2014, 23:20

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Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx

... from Catharanthus roseus Santosh Kumar, Ajaswrata Dutta, Alok K. Sinha and Jayanti Sen National Centre for Plant Genome Research, JNU Campus, Aruna Asaf Ali Marg, New Delhi, India Catharanthus ... database, i.e. Avicennia (BAB16317), Nicotiana secretory peroxidases (AAD33072), cotton (COTPROXDS) (AAA99868), barley grain (BP1) (AAA32973), Ar. thaliana (ATP 2A) A2 (Q42578) and HRP-C (AAA33...

Ngày tải lên : 23/03/2014, 09:21

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Báo cáo khoa học: Puriﬁcation and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx

... 2007) doi:10.1111/j.1742-4658.2007.05728.x A highly active glutathione S-transferase was puriﬁed from adult German cockroaches, Blattella germanica. The puriﬁed enzyme appeared as a single band of 24 kDa by SDS⁄ PAGE, and had a different ... 28, 321–329. 26 Jirajaroenrat K, Ponjaroenkit S, Krittanai C, Prapant- hadara L -A & Ketterman AJ (2001) Heterologous expression and c...

Ngày tải lên : 23/03/2014, 09:21

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