Báo cáo khoa học: Differential tissue-speciﬁc distribution of transcripts for the duplicated fatty acid-binding protein 10 (fabp10) genes in embryos, larvae and adult zebraﬁsh (Danio rerio) docx

... 2009 The Authors Journal compilation ª 2009 FEBS 6797 Differential tissue-specific distribution of transcripts for the duplicated fatty acid-binding protein 10 (fabp10) genes in embryos, larvae and ... differential tissue-specific distribution of fabp10a and fabp10b transcripts in developing and adult zebrafish, evidence of the divergence of regula-tory elements in the promoters of the fabp10a ... elements in itspromoter, as a possible process for the retention of duplicated genes in the genome. Transcripts of fabp10a were detected in liver, intes-tine and testis of adult zebrafish and only in...

Báo cáo khoa học: Site-speciﬁc phosphorylation of MCM4 during the cell cycle in mammalian cells pot

... re-replication of DNA in a single cell cycle. Inactivation of CDK1 leadsto re-replication of DNA in eukaryotic cells includinghuman cells [7]. Targets of the kinase in the regulation of DNA replication include ... presence of geminin, which inhibits the ability of CDT1 to load MCM proteins onto chromatin, mayhelp prevent re-replication of DNA in the G2 and Mphase. During interphase, chromatin-bound ... Triton X -100 used for cell fractionation and then incubated at 37 °C for 15 min in the same buffer before ﬁxation (Figs 9 and 10) .Cells were washed with NaCl ⁄ Pi and then permeabilized and blocked...

Tài liệu Báo cáo khóa học: Non-speciﬁc depolymerization of chitosan by pronase and characterization of the resultant products pptx

... scatteringrange (2h )of0 –45°. The crystallinity index (CrI, %) wasdetermined using the formula (I 110 – Iam) · 100 /I 110 ,whereI 110 is the maximum intensity at 20° and Iamis the intensity of amorphous ... convolutedspectral band around 3600–3000 cm)1 in the spectra of bothchitosan and LMWC indicated the absence of free -OHgroups and the involvement of both -OH… 3and -CH2OH…6 in intra- and intermolecular ... chains by inter- and intrachain hydrogenbonding network resulting in the formation of particle-like structure [1]. ESEM of LMWC (obtained after 5 hFig. 2. Infrared (IR) spectra of chitosanand...

Báo cáo khoa học: Mycobacterium tuberculosis possesses a functional enzyme for the synthesis of vitamin C, L-gulono-1,4-lactone dehydrogenase doc

... biosynthesis of colla-gen and other hydroxyproline ⁄ hydroxylysine-containingproteins ⁄ peptides; synthesis of secondary metabolites,hormones and cytokines [1]; oxidative protein folding and endoplasmic ... protein. The Rv1771 gene wascloned and expressed in Escherichia coli, and the corresponding protein wasafﬁnity-puriﬁed and characterized. The FAD-binding motif-containingRv1771 protein is a ... Escherichia coli. The recombinant protein contained an engineered enterokinase cleavagesite in the junction between the GST tag and the Rv1771 sequence. Upon 3 h of induction of the pDEST15_Rv1771...

Báo cáo khoa học: Stage speciﬁc expression of poly(malic acid)-afﬁliated genes in the life cycle of Physarum polycephalum Spherulin 3b and polymalatase potx

... proteins con-tain the ‘Greek key’ typical of the bc-crystallin family of proteins. While spheruline 3a like another two-domain protein, protein S [22], responds in terms of stress proteins [23] to ... bc-crystallin fold. The residues shown in grey indicate the side chains and backbone sites thatare involved in calcium binding [24]. The residues in the conserved tyrosine corners are in bold [24]. ... M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein util-izing the principle of protein- dye binding. Anal Biochem72, 248–254.N. Pinchai et al. Cell...

Báo cáo khóa học: Differential carbohydrate epitope recognition of globotriaosyl ceramide by verotoxins and a monoclonal antibody pdf

... age-related binding. Whileacetone treatment induced FITC–VT1B glomerular binding in type A samples, binding in type B samples was onlyslightly induced, indicating a robust distinction in terms of the ... FITC–VT1B binding toexplain the lack of adult renal glomerular binding.Our earlier studies showed that FITC-coupling at Lys53 of the VT1B subunit inhibited FITC-VT1B/Gb3binding[43] and that ... equivalent The binding of these three ligands to adult human renaltissue was then compared by overlay of frozen serialsections. VT1, VT2 and mAb anti-Gb3staining wereFig. 1. Binding of VT1,...

Báo cáo khoa học: Hepatocyte-speciﬁc interplay of transcription factors at the far-upstream enhancer of the carbamoylphosphate synthetase gene upon glucocorticoid induction doc

... sta-bilization of GR binding to the GRU, and therebystabilization of the interaction of the coregulatorsinteracting with the nucleoprotein complex formed at the GRU. This raises the possibility that the ... did not result in any changes in the pattern of bands for either of the two cell types, showing that binding of C ⁄ EBP and FoxA to the CPS GRU in FTO-2B cells is independ-ent of these hormonal ... the lowerstrand. FoxA binding resulted in protection of the gua-nines at positions 347 and 351 on the upper strand, and protection at position 352 on the lower strand.These footprints in the...

Báo cáo khoa học: Stage-speciﬁc activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx

... against polyhistidine revealed the same-sizedbands, suggesting that the C-terminal histidine tagremained in both the proform and activated form in the recombinant enzyme after its secretion into ... study the function of ADAMTSs during organogenesis in vivo. In this study, we isolated recombinant MIG-17 and determined the amino acid sequence of the N-ter-minal neo-epitope of the activated form, ... stage, and the activation in the gonad mostlyceased in older adults.Discussion In the present study, we expressed the MIG-17 cDNA in Sf21 cells and detected the proform and activatedform of MIG-17–6His...

Báo cáo khoa học: Stage-speciﬁc expression of Caenorhabditis elegans ribonuclease H1 enzymes with different substrate speciﬁcities and bivalent cation requirements ppt

... H1-related genes, and one of them has an isoform produced by alter-native splicing. However, little is known about the enzymatic features of the proteins encoded by these genes. To determine the differences ... completely the same,but the other proteins exhibited different patterns(Fig. 5A). On the other hand, when the RNase H assaywas performed using ﬂuorescein isothiocyanate labelingat the 3¢ end of the ... intoan RNase H1 protein that lacks a dsRHbd at the N-ter-minus. We analyzed the expression patterns of the ﬁve transcripts, including the pair of alternative splicingvariants, throughout the...

Tài liệu Báo cáo khoa học: "A semantically-derived subset of English for hardware verification" pdf

... on the syntactic context for example, whether it occurs in the antecedent or consequent of an implication. 6There is a further kind of ontological richness in English at this level, involving ... designers and therefore alternative interfaces involving graphics and natural language have been explored. In this paper, we address some of the challenges raised by converting l In practice, ... when so is selected as the initial state. States correspond to points of time in the course of a computation, and branches represent non-determinism. Formulas of CTL are either true or false...

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