Báo cáo khoa học: The tetralogy of Fallot-associated G274D mutation impairs folding of the second epidermal growth factor repeat in Jagged-1 potx

... vi-tro induced by the G274D mutation, the misfolding of the G274S and G274A mutants, the impossibility of rescuing the G274D mutation with compensatorymutations, the sensitivity of the G274D ... folded. The results obtained in the present study suggest the opposite. There areseveral lines of evidence in support of this hypothesis: the dramatic impairment of the oxidative folding in vi-tro ... Toinvestigate the effects of this mutation, we studied the in vitro oxidative folding of the wild-type and mutant peptides encompassing the second epi-dermal growth factor. We found that the G274D...

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... conformation, the SH2 domain binds to pYion the C-terminal tail, and the Src homology 3 (SH3) domain binds to the linkerbetween the SH2 and kinase domains at the back of the small lobe, preventing the ... by presenting the pulse of Src activity in the plane of the inactive and active fractions, siand s1. If the duration of the stimulus exceeds the critical value, the trajectory in the (si, ... RTKs, can bind the Src SH2 domain, facilitatingdephosphorylation of the inhibitory tyrosine pYi. The phosphatases that dephosphorylate the activating sitepYainclude the C-terminal site phosphatases,...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... composition, indicating that the peptide is of the correct sequence and free of contaminating pro-teins. Five cycles of N-terminal sequencing conﬁrmed the expected residues including the presence of methio-nine ... : 2000 in blocking solution for 30 min. Cells wererinsed in NaCl ⁄ Piseveral times and incubated in blockingserum containing anti-mouse IgG (Vectastain) for a fur-ther 30 min. Staining was ... drawback of such approachesis the cost of the afﬁnity resins used to isolate the fusion protein and the proteases required to liberate the protein of interest from the fusion protein. Suchconsiderations...

Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-speciﬁc alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt

... transcriptional initi-ation sites.Determining the translation initiation site of isoform 2 KLK11Messenger RNA for isoform 2 contains two initiationcodons near the 5¢ end. To examine whether the ﬁrstinitiation ... signalpeptide of isoform 1 (Fig. 2A) [8]. The second initi-ation codon is thought to be an actual translationinitiation site of isoform 2, instead of the ﬁrst initi-ation codon followed by 31 amino ... Predicted methioninesare shown as M. Shaded boxes show the hydrophobic region cor-responding to the signal peptide of isoform 1. In the M33S mutant, the second initiation codon in isoform 2 was...

Tài liệu Báo cáo khóa học: Active-site residues and amino acid speciﬁcity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... important for the second- half reaction and are part of the active-site of CoaB; these residues may be directlyinvolved in binding of one of the substrates, the amino acidcysteine (Fig. 3). ... cysteine. The synthesis of PPC occurs in two half reactions starting with the formation of 4¢-phosphopantothenoyl-CMP (activation of the carboxyl group of 4¢-phosphopantothenate). In a second ... important for the second- half reaction (and that isprobably involved in cysteine binding), the in vitro activity of further mutants was measured in the presence andabsence of cysteine. It turned...

Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identiﬁcation of oscillophores ppt

... cycles in such a combinationdo not touch each other). The value (the sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence ... concentrations of itsspecies. Therewith the c oefficient of o rder p of the characteristic equation equals the sum of all simplestcombined Ôminus autoinfluence paths of order pÕ in the network. ... cause instability. Then only the negative autoinflu-ence path of Eqn (16) remains, which then cancels the positive autoinfluence of Eqn (17). Revolving around the cycle then does not lead to an increase...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... (F340) increases, whereas the ﬂuorescence intensity at 380 nm (F380) decreases upon the binding of Ca2+. The change in the ratio of F340 ⁄ F380 determined the change in Ca2+concentrationinside ... Ca2+⁄CaM-binding IQ domain that is involved in the activa-tion of Ras-GRF1.Stimulation of all the splice variants of the Gs-coupledserotonin receptor 5-hydroxytryptamine7(5-HT7)increases intracellular ... of Ser916 inhibiting (Ser916Ala) or mimicking(Ser916Asp ⁄ Glu) phosphorylation did not alter these effects. However, the deletion of amino acids 1–225, including the Ca2+⁄ calmodulin-binding...

Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

... sequences of HS chains are de®ned for the bFGF binding and for the recognition of the speci®c bFGFreceptor, leading to the formation of a ternary complexcomprising HSPG±bFGF±FGFR. These oligosaccharidicmotifs ... FGF at the transcriptional l evel. The bFGFinhibitory eect was also observed in the presence of dibu-tyryl-cAMP, ch olera toxin or R O-20-1724, all inducing highlevels of cAMP, the second ... cells were incubated for 24 heither in the absence or in prese nce of FSH, dbcAMP,cholera toxin or bFGF, either in combination with FSH ordbcAMP or cholera toxin and bFGF.Extraction of total...

Tài liệu Báo cáo khoa học: Casein kinase 2 speciﬁcally binds to and phosphorylates the carboxy termini of ENaC subunits ppt

... aldosterone and insulin, as well asintracellular signalling components such as protein kinase Cand protein kinase A, were found to increase the in vivophosphorylation of the C termini of both b ... byphosphorimaging of the band corresponding to the full-length protein, and normalizing to the Coomassie bluestaining of the same band.)It was next demonstrated that the kinase eluted in fraction ... fraction in the absence of ATP. The beads containing the fusionproteins (and other proteins associated with them) wereprecipitated, washed stringently, and incubated with[32P]ATP with no added kinases....

Báo cáo khoa học: Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis docx

... Microinjection of NLS-containing substrates in a myoblast. The movie represents the NLS microin-jection experiment shown in Fig. 6A.Video S2. Microinjection of NLS-containing substrates in a ... cultured in 1.2 mgÆmL)1puromycin-containing DMEM supplementedwith 10% fetal bovine serum. After conﬁrming their abilityto differentiate, cells were maintained in 1.0 mgÆmL)1puro-mycin-containing ... GDP,across the nuclear envelope regulates the binding andrelease of cargo by transport factors. RanGTP is abun-dant in the nucleus as a result of the activity of RCC1,a guanine nucleotide exchange factor...

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