Báo cáo khoa học: Structural origins for selectivity and specificity in an engineered bacterial repressor–inducer pair pdf
Báo cáo khoa học: Structural origins for selectivity and specificity in an engineered bacterial repressor–inducer pair pdf
... positioning of the ligands and the conformations of the residues lining the bindingsite (Table 2). In an initial comparison of the ligandpositions of tetracycline, dox and atc in TetR, theseligands ... well as for all corresponding single and double mutant variants,are highly valuable for discussing the origins of speci-ficity and selectivity [8,9] (Table 3). When analyzingthe changes in the ... represent achallenging benchmark data set for testing and validat-ing computational models aimed at predicting and designing the specificity and selectivity of protein–ligand complexes.Experimental...
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Báo cáo khoa học: Structural basis for the erythro-stereospecificity of the L-arginine oxygenase VioC in viomycin biosynthesis docx
... 2009 FEBS 3673 and His316. These residues are positioned within theloop linking b-strands C and D (His168 and Glu170) and on b-strand H (His316), indicating that the iron-binding facial triad ... undergoing interactionsmainly with the C-terminal helical subdomain. Accordingly, VioC exertsbroadened substrate specificity by accepting the analogs l-homoarginine and l-canavanine for Cb-hydroxylation.AbbreviationsA-domain, ... side chain ofl-arginine, as exemplified by the binding and catalyticturnover of l-homoarginine. Concerning l-canavanineturnover by VioC, the modified guanidinium group islikely to be analogously...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... papain and actinidin, and on aminoacid sequence information for cathepsins B and H, and stem bromelain. J Mol Biol 182, 317–329.11 Carter CE, Marriage H & Goodenough PW (2000)Mutagenesis and ... of papain and ervatamin-A for residue 157 (papainnumbering, 155 for ervatamin-A) and residue 67, respectively.Table 1. Kinetic constants for peptidyl p-nitroanilides and IC50value for E-64. ... mechanism and substrate specificity and to understand the differences therein for this classof enzymes. In order to identify the subsites of theervatamins and to understand substrate or inhibitorbinding...
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Báo cáo khoa học: Probing the determinants of coenzyme specificity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis potx
... and Asp245, to coenzymebinding and specificity in P. asaccharolyticus GDH.Five mutants were created: in E243K and E243R, theP7 glutamate was replaced by positively charged lysine and arginine, ... coenzyme binding was tighter and thereforemeasurable and, in particular, gave measurable Kdvalues for binding of NADPH to both E243K and E243R, whereas the binding of NADH to thesemutants was ... E243R and E243K, reflecting thecombination of improved efficiency with NADPH and diminished efficiency with NADH. This is muchgreater than the shift in discrimination values for D245K, E243D and...
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Tài liệu Báo cáo khoa học: Structural bases for recognition of Anp32⁄LANP proteins doc
... domain.Stabilizing interactions could form between His230 ofPP2A-C and Asp119 and Asn94 of Anp32a. A saltbridge could form between Glu226 of PP2A-C and Lys67, Lys68 and Lys91.Testing the interaction ... subnuclearstructures. The interaction was mapped onto the LRR and AXH domain of Anp32 and Atx1 respectively, and was shown to be stronger for expanded Atx1[16,18]. The temporal and cell-specific expressionpattern ... down and washed threetimes with RIPA buffer before being resuspended in SDS ⁄ PAGE sample buffer. Samples and input controlswere analyzed by PAGE and western blotting using mono-clonal antibodies...
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Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt
... maltodextrin-binding protein ( TvuCMBP).The bacterial sugar-binding protein is a member ofthe bacterial ATP-binding cassette transport system, and the mechanism of the maltodextrin transport in Escherichia ... proteinsAs the sugar-binding site is formed by N- and C-domains, residues involved in sugar binding aregrouped into two parts, the N-domain part and theTable 1. Data collection and refinement statistics.Derivative ... searched for using the DALI server [25], and many bacterial sugar-binding proteins and otherperiplasmic binding proteins were listed. In this search,TvuCMBP most resembled a closed form of EcoMBPcomplexed...
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Báo cáo khoa học: Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase doc
... lgofc1purified in detergent, the slower migrating bandreappeared (lanes 4 and 8). Upon incubation of 2 lg of pure c11with 2 and 10 lgofc1purified in chloroform ⁄ methanol, the slowermigrating c ring ... c subunit, and in accordance withthe binding change mechanism [3], this arrangementsuggests a catalytic mechanism in which the c subunitrotates within the a3b3cylinder. Elegant experimentshave ... obtainpure c ring, as described in the Experimental procedures (lanes 2 and 5). As a control, c-ring bands migrating more slowly were cutout and electroeluted (lane 3). Upon incubation of 2 lg of pure...
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Báo cáo khoa học: Structural basis for recognition of Co2+ by RNA aptamers pot
... G28 or G37 and G38 in aptamer no. 18, as well as G25 and G26 orG39 and G40 in aptamer no. 20, in Co2+binding.Interestingly, a similar metal ion binding mode tothe tandem guanines has been ... determined j values were verysimilar: 4.0 and 3.6 for G27 and G28 in aptamerno. 18 and 3.7 and 3.5 for G25 and G26 in aptamerFig. 3. Iodine cleavage analysis of phospho-tioate nucleoside analogs ... sitesremain available for interactions with RNA ligands,including the N7 atom of purines. Therefore, there isthe possible involvement of the N7 atom of the neigh-boring tandem guanines, G27 and...
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Báo cáo khoa học: Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase potx
... Enzyme–MeGlcA2Xyl3interactions (for clarity,Ser258, forming a hydrogen bond to MeGlcA, is not shown). Ligands and amino acids involved in ligand binding are in ball -and- stick represen-tations (atoms: ... a)8-barreldomain is in red, the C-terminal b9-barrel domain is in blue, the con-necting region is in orange, the catalytic amino acids Glu165 and Glu253 are in ball -and- stick representations, and MeGlcA2Xyl3, ... Characterization of the binding site surface and the list of the interactions between E. chrysanthemiGH30 xylanase and MeGlcA2Xyl3ligand.This supplementary material can be found in theonline version...
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Báo cáo khoa học: Structural requirements for antimicrobial versus chemoattractant activities for dermaseptin S9 pptx
... (2852 and 2923 cm)1) assignedto the symmetric and antisymmetric methylene stretchingmodes, and weaker bands (2875 and 2956 cm)1) assigned tothe symmetric and antisymmetric methyl stretching ... of the mem-branes using an Alpha Innotech image analyser and theAlpha Innotech fluorchem 8800 (San Leandro, CA, USA)software. To differentiate between chemotaxis and chemo-kinesis, experiments ... 4143processes, and those with functional implications in vivo[49]. For example, both human Pmel17, which plays an important role in the biosynthesis of the pigment mela-nin, and the factor XII protein...
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