... for the optimal activity of
BsArb43B.
Furthermore, to determine whether the role of the
calcium is structural, thermal shift assays were per-
formed to determine the T
m
of the protein in the ... optimizing binding of the carbohydrate chain
in the cleft, and resulting in trioses as products. These
observations suggest that, by blocking one of the ends...
... doi:10.1046/j.1432-1033.2003.03554.x
hypothetical cascade of events initiated by ouabain occu-
pancy of a small fraction of the sodium pumps. One of them
had advocated the role of EDLF for years [15].
Possible pitfalls for a ... general rule is the almost ouabain-insensitive a
1
-isoform
seen in rats ( [for references, see [18]). In most tissues in the
rat, howe...
... c
10
complex in the recombinant c ring
preparations suggests that the insertion of the last c
subunit forms the limiting step in the assembly process
of a functional oligomer.
Upon closer inspection, the ... to
clarify whether the stoichiometry of the c rings is
in uenced by variation of the expression level of
subunit c.
In the present study we investigat...
... that
the 4¢-phosphopantetheine moiety of the peptidyl carrier protein changes
its position as a result of a conformational change in the A domain, which
is induced by the binding of an amino acyl ... phenylalanine are not suitable because their
use would lead to the formation of the amino acyl
thioester on the Ppant of the PCP domain and prime
the enzyme for t...
... 0.01).
TBP-promoter induced conformational change in CYIIB
We then examined the effect of TBP–AdML binding on the
FRET efficiency observed for CYIIB (Fig. 3A). The ratio
of the intensity between the peak of 526 ... accelerates the formation of a
TFIIB–TBP–DNA complex
To gain insight into the kinetics of the TFIIB–TBP–
promoter complex formation, we investigated t...
... parallel corpus of two languages and al-
most parse information for the sentences of one
of the languages, one can rapidly develop a gram-
mar for the other language using supertagging, as
suggested ... For example, in the sentence "Many Indians
]eared
their country
might
split again" the pres-
ence of
might
influences the choice of the supe...
... ap-
peal of these methods is that their training criterion
is often discriminative, attempting to explicitly push
the score or probability of the correct structure for
each training sentence above the ... split into
5 sections, and in each case the maximum-entropy
tagger was trained on 4/5 of the data, then used to
decode the remaining 1/5. The top 20 hypotheses
under...
... explained by the proximity of the
mutation to the cleavage site, probably resulting in a
small interference with the binding of the activator to
the FXI zymogen.
There are some examples of inherited ... (1993)
The linkage between binding of the C-terminal domain
of hirudin and amidase activity in human alpha-throm-
bin. Biochem J 289, 475–480.
45 Baglia FA &...
... Currently, there
are approximately 60 grammar rules defined for
Greek, allowing for the complete parse of about
50% of the sentences in a corpus like Europarl
(Koehn, 2005), which contains proceedings of
the ... contains proceedings of
the European Parliament. For the remaining sen-
tences, partial analyses are instead proposed for
the chunks identified.
One of...
... leading to the preferential hydration of the
protein domain is the driving force of protein stabiliza-
tion or folding [3,4]. Furthermore, the effect of
osmolytes on the functional activity of ... DG
D
° values in the
presence of these osmolytes are given in Table 1. How-
ever, DG
D
° values of RNase-A in the presence of
alanine, serine, lysine, b-alanine, ta...