... molecule binds at three subsites in the polysaccharide binding site of the TSbH domain of HylP2. The positions of ligandsare indicated at the substrate binding groove. (B) The view from the top ... molecules, indicating the strong nature of the binding of ascorbic acid to proteins. Althoughascorbic acid is a small molecule, it blocks the mostattractive binding subsites in the protein, leading ... examination of theseregions indicates that PheC175 protrudes into the sub-strate binding area between subsites 1 and 2. Thus, ithampers the attachment of ligand at this subsite. Simi-larly, the...