... MPH a Forward: 5¢-TAGAATTCGCTGCTCCACAAGTTAGAACT-3¢Reverse: 5¢-TAGCGGCCGCTTACTTTGGGTTAACGACGGA-3¢Mutant MPHbG194P 5¢-CCTGACGATTCTAAACCGTTCTTCAAGGGTGCC-3¢G198P 5¢-AAAGGTTTCTTCAAGCCGGCCATGGCTTCCCTT-3¢G194P ... was isolated fromthe soil at a pesticide factory in Tianjin, China, andstored in our laboratory [7]. The E. coli strains JM109(Promega, Madison, WI, USA) and BL21 (DE3) (Nov-agen, Darmstadt, ... Each test wascarried out with at least three replicates. The Kmand kcatval-ues were calculated by nonlinear regression using graphpadprism 5.0 (GraphPad Software Inc., La Jolla, CA, USA).Thermostability...