Báo cáo khoa học: Cellulose crystallinity – a key predictor of the enzymatic hydrolysis rate pptx
Báo cáo khoa học: Cellulose crystallinity – a key predictor of the enzymatic hydrolysis rate pptx
... crystallinity rather than adsorption on the enzymatic rate. Thus, the cellulase activity and initial rate data obtained from varioussamples may provide valuable information about the details of the ... compilation ª 2010 FEBS Cellulose crystallinity – a key predictor of the enzymatic hydrolysis rate Me´lanie Hall, Prabuddha Bansal, Jay H. Lee, Matthew J. Realff and Andreas S. BommariusSchool of ... accessibility, and therefore the hydrolysis rates [29,40,41].Another important criterion related to hydrolysis rate involves the adsorption capacity of cellulases onto cellulose. The rate of hydrolysis was...
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Báo cáo khoa học: Calpain 3: a key regulator of the sarcomere? pot
... demonstrated the safetyand efficacy of adeno-associated virus (AAV)-mediatedcalpain 3 cDNA transfer in a mouse model of LGMD 2A [26]. However, gene therapy still has someobstacles that remain to ... Sorimachi H (1998) A novel aspect of cal-pain activation. FEBS Lett 433, 1–4 .12 Sorimachi H, Toyama-Sorimachi N, Saido TC,Kawasaki H, Sugita H, Miyasaka M, Arahata K,Ishiura S & Suzuki K (1993) ... that cytoskeletal proteins are one class of its substrates.Through the absence of cleavage of these substrates, calpain 3 deficiencyleads to abnormal sarcomeres, impairment of muscle contractile...
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Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner doc
... (forward), 5¢-agaacgcatgcagccgaggg-3¢ (reverse); KS2 5¢-tggtagctacctgggaagcc-3¢ (forward), 5¢-gaagcaccagactcattctg-3¢ (reverse);MACS1 5¢-gagttggagctccaagctgg-3¢ (forward), 5¢-tgatccctgttcgcatgcag-3¢ ... toproduce acyl-CoA on the outer membrane of mitochondria.Acyl-CoA is then transported into the matrix forb-oxidation of acyl-group. Among the MACS familyproteins with the acyl-CoA synthetase activity, ... (5¢-GAATTCatgaaggttctcctccactg-3¢)and rO-MACS-XhoI-D (5¢-CTCGAGtgcccgtccccactcctggt-3¢)for o-macs; EcoRI-mMACS1-U (5¢-GAATTCatgcagtggctgaagagttt-3¢) and mMACS1-XbaI-D (5¢-TCTAGAtagctgaccaaactccttg-3¢)forMACS1, and cloned...
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Báo cáo khoa học: ERS1 encodes a functional homologue of the human lysosomal cystine transporter pptx
... also assayed LysoSensor greenstaining of a vma1D strain, which lacks the 118-kDasubunit of the V-ATPase (Fig. 5A) and is defective invacuolar acidification. Compared to the ers1D strain(data ... cells thatlack Meh1, the vast majority of Gtr1 appeared soluble,fractionating in the S16 supernatant. These results dem-onstrate that the membrane association of Gtr1 isdependent on Meh1 and ... ers1Dphenotype (data not shown). These observations sug-gest that the accumulation of cystine in the vacuole of yeast does not lead to the types of cellular damage thatis observed in mammalian cells....
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... 27693QEGYGSTQLPSDEPLNGLNDCAA GAA GGA TAT GGC AGC ACC CAA CTT CCA TCG GAC GAG CCA TTG AAC GGG CTC AAC GAC 336113KAIQDILNEHNMFRAKERVPAAG GCC ATA CAG GAC ATC CTC AAC GAG CAC AAC ATG TTC CGC GCC AAG GAG CGC GTC CCG ... GAG CAG GTA GCG TCC GAG TCA GCC TCC CTC ACA ACA CCA 21673TTREEQPAAEAAASTAGGSQACC ACC AGG GAA GAA CAA CCG GCG GCA GAG GCC GCG GCG TCC ACC GCT GGC GGT AGC CAA 27693QEGYGSTQLPSDEPLNGLNDCAA GAA ... 516173SGAISWKTTVDTWSDEKKSYTCC GGC GCC ATC TCC TGG AAG ACG ACG GTG GAC ACG TGG AGC GAC GAG AAG AAG AGC TAC 576193HYGSNTCDQGKMCGHYTAVVCAC TAC GGC TCC AAC ACC TGC GAC CAA GGC AAG ATG TGC GGC CAC TAC...
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Báo cáo khoa học: Slc12a2 is a direct target of two closely related homeobox proteins, Six1 and Six4 docx
... basement membranes. Histochem Cell Biol 113,11 5–1 24.52 Nagahama H, Hatakeyama S, Nakayama K, Nagata M,Tomita K & Nakayama K (2001) Spatial and temporalexpression patterns of the cyclin-dependent ... oligocDNA probe and a sense cDNA probe (complementary to the antisense) for mouse Slc1 2a2 mRNA were designed asfollows; Slc1 2a2 antisense, 5¢-ATCTTCACAAGAAAAATCACCTGGTACCAAGGATGT; Slc1 2a2 sense, ... 15 1–1 64.26 Tomari S, Nagahama H, Shu Y, Hoshi S, NakayamaK, Nakayama KI & Nagata M (2002) Glomerular dif-ferentiation in p27 and p57 double-mutant metanephroi.Anat Embryol 206, 3 1–3 6.27...
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Báo cáo khoa học: "PREDICTIVE COMBINATORS A METHOD PROCESSING OF COMBINATORY CATEGORIAL GRAMMARS" doc
... 198 5a. Natural Language Parsing with Combinatory Categorial Grammars in a Graph- Unification-Based Formalism. Ph.D. disser- tation, University of Texas at Austin.[Some of this material is available ... suspects that the generative capacity of the gram- mars that Steedman assumes, say, for Dutch, is in the same class with Tree Adjoining Grammars (Joshi 1985) and Head Grammars (Pollard 1984). ... indicates that the argument Y must appear to the right of the functor, as in X/Y; a left- slanting slash indicates that the argument Y must appear to the left, as in X\Y. 5 A vertical slash...
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Báo cáo khoa học: MicroRNA-373, a new regulator of protein phosphatase 6, functions as an oncogene in hepatocellular carcinoma pdf
... worksimage acquisition and analysis software was used to quan-tify band intensities. Antibodies were purchased from Tian-jin Saier Biotech and Sigma-Aldrich.Statistical analysisData are expressed ... LA,Sjoblom T, Barad O, Bentwich Z, Szafranska AE, Lab-ourier E et al. (2006) The colorectal microRNAome.Proc Natl Acad Sci USA 103, 368 7–3 692.14 Yanaihara N, Caplen N, Bowman E, Seike M, Kumam-oto ... predictmiRNA targets, which are based mainly on base pair-ing of miRNAs and the 3¢-UTRs of genes [6,2 4–2 6].Twelve candidate genes were predicted by three bioin-formatics software packages (pictar, targetscan,and...
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Báo cáo khoa học: Broad antibiotic resistance profile of the subclass B3 metallo-b-lactamase GOB-1, a di-zinc enzyme potx
... reverse:(5¢-GATCTTGCTGCTTACTGCGGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCCGCAGTAAGCAGC-3¢)For the Q116N mutant forward and reverse:(5¢-GATCTTGCTGCTTACTAACGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCGTTAGTAAGCAGC-3¢)For ... reverse:(5¢-GATCTTGCTGCTTACTAACGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCGTTAGTAAGCAGC-3¢)For the Q116H mutant forward and reverse:(5¢-GATCTTGCTGCTTACTCATGCTCACTACGACCATACAGG-3¢)(5¢-GCACCTGTATGGTCGTAGTGAGCATGAGTAAGCAGC-3¢)Production and ... substrate. In these cases, the kcatvalues were obtained frominitial hydrolysis rates measured at saturating substrateconcentrations. All data were analysed using MicrosoftExcel and the kaleidagraph...
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Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx
... in the presence of antisense primer SB1-20 (5¢-TCAAACCACACCCCGAGAAA-3¢)and[32P]dATP[aP](3000 CiÆmmol)1; Amersham Pharmacia Biotech.), follow-ing the manufacturer’s instructions. The labeled ... shown are the mean (j) and standard deviation (bars) for each cRNA a: b ratio, and the sample size percRNA combination is shown in parenthesis. Data are from five different batches of oocytes, each ... MW.Custom-made software and hardware were used for acquisition andanalysis of data. Leakage and linear capacity currents weresubtracted by using P/4 protocols. Data were sampled onceevery 5 ls and...
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