... the yeast cytochromebc1 complex based on analysis of single and double deletion mutantslacking supernumerary subunits and cytochromebVincenzo Zara1, Ilaria Palmisano1, Laura Conte1and ... Electro-phoretic analysis of DNA on agarose gels, restrictionendonuclease analysis, ligation of DNA fragments,Table 1. Yeast strains used in this study.Strain Genotype ReferenceW303–1 A (WT) MATa, ade2–1, ... genedeletion in Saccharomyces cerevisiae. Nucleic Acids Res. 21,3329–3330.22. Ito, H., Fukuda, Y., Murata, K. & Kimura, A. (1983) Transfor-mation of intact yeast cells treated with alkali cations....