Báo cáo khoa học: The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii potx

... ligands of the Fe-S cluster. Consequently, the binding of the ATPanalog and the subsequent rearrangement of the inter- domain interaction affects the electronic condition of the Fe-S cluster ... with the reconstitution of Fe-S clusters in aerobically puriﬁed apo -VnfA support the presence of [Fe3S4]+ in VnfA. To obtain further evidencedemonstrating the involvement of the Fe-S cluster ... mono-mer, as well as the stoichiometry of four Fe-S clusters in native VnfA. Functional analyses of the Fe-S cluster To clarify the roles of the Fe-S cluster found in VnfA, we performed in vivo assays...

Tài liệu Báo cáo khoa học: An immunomodulator used to protect young in the pouch of the Tammar wallaby, Macropus eugenii pptx

... production of inter-leukins and secretion of immunoglobulins on the mucosal surface of the intestine [49,50]. The antibacte-rial defence of the intestinal mucosa depends in parton stimulation of CCK ... euge-nin, indicating that eugenin is indeed acting throughCCK2receptors.To further investigate the interaction of eugenin withCCK2receptors, we investigated the effect of eugeninon lymphocyte ... concerning the sequence from the C-terminal end of the peptide, while the Y+2 fragmentations (shown schematically under-neath the spectrum) provide sequencing data from the N-terminal end of the...

Tài liệu Báo cáo khoa học: "Using adaptor grammars to identify synergies in the unsupervised acquisition of linguistic structure" docx

... grammar, in which the subtrees are specified in advance, in an adaptor grammar the subtrees, aswell as their probabilities, are learnt from the train-ing data. In order to make parsing and inferencetractable ... parse of thatstring with a PCFG approximation to the adaptorgrammar. This PCFG contains a production for eachadapted subtree in the parses of the other strings in the training corpus. A final ... grammars relax399this independence assumption by in effect learning the probability of the subtrees rooted in a specifiedsubset M of the nonterminals known as the adaptednonterminals.Adaptor grammars...

Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

... that the RPEL domain of MRTF-A bindsactin more strongly than the RPEL domain of myocar-din, and that the RPEL motif itself is an actin-bindingelement. RPEL1 and RPEL2 of myocardin bind actinweakly ... asSTARS-interacting proteins. These novel proteins con-tain four LIM domains and a C-terminal villin head-piece domain, which mediates actin-binding in severalproteins, such as villin and dematin ... cell lines [94].Table 1. Role of nuclear actin-binding proteins interacting with the androgen receptor. AR, androgen receptor; LBD, ligand-binding domain. Actin-bindingproteinTargetingsequence...

Báo cáo khoa học: "Some Comments on Algorithm and Grammar in the Automatic Parsing of Natural Languages" ppt

... written in the form of appropriate tables of rules. The paper raises the question of the increasing complexity of the tables when more than the most elementary natural-language con- ditions are included, ... machine translation. It must be noted at the outset that in this author’s opinion the aim of the automatic-parsing component of a machine-translation program is the adequate recognition of the ... Examples of this are very common in English and include many of the frequently cited instances of nesting. The second consideration is that the determination of external functioning by con-...

Báo cáo khoa học: A novel serine protease highly expressed in the pancreas is expressed in various kinds of cancer cells potx

... conﬁrm the expression of proseminmRNA in some cell lines. A band of the predicted size(500 bp) of the PCR product was observed for most of the cell lines tested, including HPC-YO and HPC-YS, in ... mole-cular mass from 33 000 to 31 000, indicating successfuldigestion of the artiﬁcial trypsinogen pro-enzyme frag-ment. The amount of 31 kDa prosemin increased in pro-portion to the time of incubation ... cancer cell lines.Ovarian cancers express some kinds of chromosome 16serine proteases, including testisin and prostasin. Weinvestigated the expression of prosemin in ovarian can-cers using immunohistochemistry....

Báo cáo khoa học: Two CCAAT/enhancer binding protein sites in the cytochrome P4503A1 locus Potencial role in the glucocorticoid response ppt

... dexamethasone is aninducer of C/EBPa, exerting a positive effect on the steady-state levels of both the mRNA and protein levels in the liver. The increase in the levels of both hepatic C/EBPa isoforms clearly ... C/EBPresponsiveness to the thymidine kinase promoter of the pT81Luc recombinant. We performed the cotransfection of CHO cells with pMSV/EBPa and single recombinantscontaining two or three synthetic copies of 3A1-300 ... precedes induction of the CYP3A1mRNA, suggesting that these factors may play an active role in the transcriptional activation of the CYP3A1 gene bydexamethasone. In vivoCYP3A1-C/EBPa binding activityWe...

Báo cáo khoa học: FLIP and MAPK play crucial roles in the MLN51-mediated hyperproliferation of ﬁbroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis pdf

... critical role in the proliferation of MH7A cells. In order to determine whether MLN51 is involved in the anti-apoptosis as well as cell proliferation of MH7A in response to GM-CSF, we examined the apoptosis ... followed by the blocking of FLS apoptosis.FLIP upregulated by MLN51 plays a crucial role in the anti-apoptosis of MH7A cellsWe examined whether FLIP was involved in the anti-apoptosis of MH7A ... animportant role in the post-translational modiﬁcation of MLN51, resulting in the protection of MLN51 from ubiquitin-mediated degradation. In summary, in RA FLSs: (1) GM-CSF signalingactivates...

Báo cáo khoa học: Functionally distinct dopamine and octopamine transporters in the CNS of the cabbage looper moth* potx

... dopamine-selective transporter is found.Keywords: insect; neurotransmitter; transporter; dopamine;octopamine; cocaine. The catecholamine dopamine (DA), the phenolamineoctopamine (OA) and the indolamine ... does the moth OAT substitute for DAT in dopami-nergic signaling pathways in the moth CNS, and what is the nature of the mechanism that substitutes for OAT in the octopaminergic pathways in the ... conﬁrmed these proteinsare produced from distinct genes by characterization of theirexpression using Northern analysis and in situ hybridiza-tion. The pattern of DAT RNA expression in the caterpillarCNS...

Báo cáo khoa học: Upstream and intronic regulatory sequences interact in the activation of the glutamine synthetase promoter pot

... (muscle). The application of the ANOVAtechnique allowed us to segregate the main(intrinsic) activities of the respective elements from the effects of their interactions. Using this approach, the upstream ... sequences present in the respective constructsare shown in the left portion of the figure as solid black lines. The upper line shows the linkage of the 5.3-kb genomic GS segment with the luciferasereporter ... protein concentration (bicinchoninic acid reagent;Pierce).Splicing of modified first intronConstructs carrying the different modules of the GS firstintron were tested for proper splicing of the...

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