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Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

... FEBS Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron- encoded protein contribute to its maturase and intron DNA-insertion functionMarı´a D. Molina-Sa´nchez, ... that the C-tail of the RmInt1 intron- encoded protein contributes to the maturase function of this reverse transcriptase protein. Furthermore, within the C-terminal region, we identified, in apredicted ... suggestedthat the N-terminus of the RT domain is required for protein interactions with the high-affinity binding site in subdomain DIVa of the intron, whereas otherregions of the RT and domain X interact...
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Báo cáo khoa học: Structural diversity in lipopolysaccharide expression in nontypeable Haemophilus influenzae Identification of L-glycero -D-manno-heptose in the outer-core region in three clinical isolates potx

Báo cáo khoa học: Structural diversity in lipopolysaccharide expression in nontypeable Haemophilus influenzae Identification of L-glycero -D-manno-heptose in the outer-core region in three clinical isolates potx

... 1209.Structure of the core region of the Hep4-glycoforms. In the 1H NMR spectrum of OS-1¢-B (Fig. 5C), anomericresonances of the three heptose residues (HepI–HepIII) in the inner-core region were ... acetylationat O-4 of GlcI and at O-3 of HepIII, as observed for the Hep3-glycoforms (see above). From the combined data, the structure in Scheme 4 is proposed for the Hex5 glycoform in the Hep4-glycoform ... (I, II and III) and t-Hex-Hex-Hex (I and III), respectively. Isomer III could bederived from the ion pairs at m/z 753.3/1145.3 (cleavageHepI–HepII) and 1001.5/897.5 (cleavage HepII–HepIII).Isomer...
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Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... FEBS 3759IntroductionC-peptide is derived from most of the proinsulin seg-ment in between the B and A chains of insulin [1] and has an important structural role in the proper folding and disulfide ... in the presence of trifluoroethanol [15]. In addition, molecular dynam-ics simulations propose turn-like motifs in the mid- region and in the C-terminal region [16]. The ability of peptides and ... distribu-tion of 100 lM proinsulin C-peptide in the presence of divalent Ca2+ and Mg2+ionsunder native conditions (B), and of 100 lMC-peptide in the presence of insulin (C) and of 100 lM...
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Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation pptx

Báo cáo khoa học: SAF-3, a novel splice variant of the SAF-1/MAZ/Pur-1 family, is expressed during inflammation pptx

... splicing. Insertion of a newexon originating from the non-coding sequences of intron 4 of the SAF-1/MAZ gene resulted in creation of a different C-terminus consisting of eight zinc fingerdomains ... binding of the N-terminus by ancillaryfactors, (c) the lack of a negatively functioning modulethat is present in the N-terminus of SAF-1 [26], or (d)all of the above.It was interesting to ... and increase its DNA-binding and transactivating func-tions. The SAF-1 DNA-binding and transcriptionalactivity is significantly increased in response to media-tors of signal transduction and phosphorylation...
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Báo cáo khoa học: A simple in vivo assay for measuring the efficiency of gene length-dependent processes in yeast mRNA biogenesis doc

Báo cáo khoa học: A simple in vivo assay for measuring the efficiency of gene length-dependent processes in yeast mRNA biogenesis doc

... plasmids containing the PHO5coding region transcribed under the control of the GAL1 promoter, but differing in the length of their3¢-UTR. To increase the length of the 3¢-UTR weinserted Escherichia ... presence of sublethal amounts of MPA. (B) mRNA levels of the transcription units and strains ana-lyzed in (A) cultured in the absence of MPA. The relative values of the mRNA levels with respect to the ... mutants,affected in the large subunit of yTFIIE [31]; and twomutants, srb10D and srb11D, lacking subunits of the cyclin–kinase complex that negatively regulates tran-scription initiation [32,33]. None of them...
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Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx

Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx

... of the AP dimer showing the spin-labeled sites. The spin-labeled sites comprised S53, C67, S78, S80,S337 and S373. The other subunit binds to the left of the subunitshown and in front of the ... The S80 position allows the nitroxide spin-label placed there to point into the active site close to the S65 and in the direction of the metal ions. An almost complete reactivation of S78R1 and ... steric interaction between the spin-label and the side chain at position 274. The results of the EPR indicate a reduction in the rate of movement of the R1 attachment-point (DH0)1compo-nent in...
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Báo cáo khoa học: What’s in a covalent bond? On the role and formation of covalently bound flavin cofactors doc

Báo cáo khoa học: What’s in a covalent bond? On the role and formation of covalently bound flavin cofactors doc

... B. The isoalloxa-zine ring of FAD is in yellow. The axis of the pink helix pointsdirectly at the C2-O of the isoalloxazine. Cys397, covalently bound to the 8a-carbon of the isoalloxazine ring, ... differenceswithin one type of binding fold. With regard to the latter, in most VAO-type covalent flavoproteins, the flavin is covalently linked to an amino acid that ispart of the FAD-binding domain, whereas ... formation and protein oligomerization.For a richer understanding of the role of covalentflavin binding to proteins, the scope of the researchperformed in this area should be broadened. Establish-ing the...
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Báo cáo khoa học: Cell type-specific transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt

Báo cáo khoa học: Cell type-specific transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt

... study was to investigate the intracellular fate of PrPCby examining for the firsttime its biosynthesis in the secretory pathway of neuro-endocrine cells in vivo and the effect of the transgeneexpression ... suggesting that the expression of the GFP–PrPCfusion protein in the inter-mediate pituitary is coregulated with these proteins. The POMC and p24d1 ⁄ 2 protein levels were similar in the ALs of ... December2005)doi:10.1111/j.1742-4658.2006.05118.x The cellular form of prion protein (PrPC) is anchored to the plasma mem-brane of the cell and expressed in most tissues, but predominantly in the brain, including in the pituitary gland....
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Báo cáo khoa học: LIN54 is an essential core subunit of the DREAM / LINC complex that binds to the cdc2 promoter in a sequence-specific manner ppt

Báo cáo khoa học: LIN54 is an essential core subunit of the DREAM / LINC complex that binds to the cdc2 promoter in a sequence-specific manner ppt

... reported the binding of E2F4 and p130 to the CDE element in G0 [22]. Binding of LIN54 to the adjacent CHRelement could therefore stabilize the binding of E2F4 ⁄ p130 at CDE. To address whether the ... importantfor the binding of LIN54 to the cdc2 promoter(Fig. 5C).We next asked whether the same element wasinvolved in the binding of a LIN54-containing com-plex from cellular lysates. To address ... used to compete for the binding of the GST protein to the labeled probe (+).Labeled DNA was detected by autoradiogra-phy. The positions of the free probe and the shifted GST-CXC protein are indicated.F....
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Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt

Báo cáo khoa học: Mycobacterium tuberculosis ClpC1 Characterization and role of the N-terminal domain in its function ppt

... N-domain, homologous to the N-domains of ClpA or ClpB, and a linkerdomain homologous to, but half the size of, the linker domain of ClpB [19]. The N-terminal region contains two 32-amino acid ... Clpfamily of proteins in survival and virulence, it is of interest to understand the mode of action of theseproteins in M. tuberculosis. In this study, we functionally characterized the ClpC1 protein ... rendered the protein more sta-ble. In ClpC1D1, the N-terminal repeat II is extendedby 40 amino acids of the linker sequence betweenrepeats I and II. In ClpC1D2, the N-terminal conservedrepeat II...
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