... taggedprotein. Merging of the images indicated that most of the puncta of CFP–Atg8L were colocalized with those of YFP–LC3 (Fig. 4,f,I,l). In the absence of inhibitors,only a few puncta of either type were ... [21]. The aminoacid sequence of mouse Atg8L ⁄ Apg8L shows 100% and 54% identity to those of human Atg8L and yeastAtg8, respectively, and 33%, 60% and 86% identity to the amino acid sequences of ... cleavage of the C-terminus of Atg8L. Wild-typeTRX–Atg8L)3xFLAG was expressed in Escherichia coli; the cellswere lysed and centrifuged, and the supernatants were used as the substrate. The supernatants...