... NuijensPharming, Archimedesweg, Leiden, the NetherlandsLactoferrin (LF) is an iron-binding glycoprotein of the innate host defence system. To elucidate the role of N-linkedglycosylation in protection of ... (10)3· Mr) indicate the migration of the protein standards. hC2-tryp, hC1-tryp and hN1-tryp represent the tryptic C- and N-lobefragments of hLF bearing either 2 or 1 N-linked glycans.680 ... SDS/PAGE(12.5%). bLF-A (lanes 1, 3 and 5) and bLF-B (lanes 2, 4 and 6); after 0,30 and 240 min of digestion, respectively. bC3-tryp, bC3,bN2-tryp and bN1-tryp indicates the tryptic C- and N-lobe fragments...