Solar and Space Physics and Its Role in Space Exploration doc

Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx

... mutations in the AUH gene. This indi- cated that AUH, in addition to its RNA binding func- tion, must play an important role in leucine catabolism. AUH was overproduced in E. coli and characterized by ... of leucine catabolism at the protein and DNA levels and developed a novel assay for enzyme analysis in a diagnostic setting. The human AUH protein was first recognized by...
Ngày tải lên : 19/02/2014, 07:20
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Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx

Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx

... digest- ible protein substrate, and finally calpastatin. The finding that calpastatin was the most efficient ligand at promoting calpain transition, detected as an increase in mAb that bound to calpain, is ... the intensity of calpain staining increased 7– 8-fold, indicating that the protease was now mainly present in the high-affinity form. In these conditions, although the highest amoun...
Ngày tải lên : 07/03/2014, 12:20
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... with GST-CD2 (MAP2 v-KIND binding core). Input (cerebellum) GST v-KIND KIND1 v-KIND KIND2 Spir-2 KIND PTPN13 KIND Input (MAP2) GST v-KIND KIND1 v-KIND KIND2 Spir-2 KIND PTPN13 KIND PD from cerebellar ... stain) EGFP EGFP-KIND2-1 EGFP-KIND2-2 EGFP-KIND2-3 EGFP-KIND2-4 EGFP-KIND2-5 EGFP-KIND2 EGFP-KIND1 KIND2-1 487 KIND2-2 521 KIND2-3 555 KIND2-5 589 KIND2-4 488 456 620 KIND2 v-KIND + + + – – I...
Ngày tải lên : 14/02/2014, 19:20
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Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx

Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx

... findings, our research provides new insights into the role of exon 9 in the regulation of apoptosis and invasion in liver cell lines. A B Fig. 7. Effects of occludin shRNA on apoptosis and invasiveness. ... level increased in Occ WT -overexpressing cells, and that 2-APB inhibited the effects of Occ WT . Therefore, the increase in intracellular calcium may explain the induction...
Ngày tải lên : 18/02/2014, 18:20
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

... places, but in particular, stefin B has a number of histidines in the C-terminus (box in Fig. 1) at sites: 92, 75, 66 and 58. As histidine residues are central to copper binding in many proteins they ... competitor to nonspecific protein copper interactions. A control ITC experiment of titrating stefin B protein with glycine alone was performed and no binding was observed. In our h...
Ngày tải lên : 19/02/2014, 06:20
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Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc

Tài liệu Báo cáo khoa học: Inorganic pyrophosphatase in the roundworm Ascaris and its role in the development and molting process of the larval stage parasites doc

... P i released min )1 Æmg )1 of protein. Enzyme kinetic study The K m (Michaelis constant) and V max (maximum velocity) values were determined by incubating the diluted recombin- ant proteins in the standard ... at 26 000 g for 30 min at 4 °C. The supernatants containing recombinant proteins of AsPPase were purified using ProBond TM resin (Invitro- gen) under nondenaturing conditions and s...
Ngày tải lên : 20/02/2014, 11:20
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

... a protein containing a single ankyrin repeat that interacts with HIV-1 viral protein R (Vpr) and we designated this protein as Vpr-binding ankyrin repeat protein (VBARP). This interaction was ... aprotinin, and protease inhibitors leupep- tin, chymostatin, and pepstatin (1 lgÆmL )1 ; Sigma) as des- cribed [14] for 15 min in ice. Cell lysates were centrifuged at 22 000 g for 10 min at...
Ngày tải lên : 07/03/2014, 21:20
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Tài liệu Corporate Ownership structure and the Informativeness of Accounting Earnings in East Asia doc

Tài liệu Corporate Ownership structure and the Informativeness of Accounting Earnings in East Asia doc

... (Jacobson and Aaker, 1993; Ali and Hwang, 2000). In addition, instead of using NI in our regression model, we have also used ∆NI, change in earnings (current earnings minus lagged earnings all ... complex earnings-generating process, may have weaker earnings-return relations than firms operating in a single industry. 15 Finally, we include firm size as a control for other missi...
Ngày tải lên : 18/02/2014, 01:20
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt

Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt

... (4NPGlc), incubating for 30 min at 37 °C, and measuring the rel ease of 4NP. Protein assays and protein sequencing Total protein in crude and semipuri®ed samples was estimated using the Pierce Protein ... ncreasing D t o 2 0% (10 min), 50% ( 15 min), 100% (5 min), held at 100% (5 min); cyanodin glycosides; 5% B/95% A initial (5 min), increasing B to 20% (10 m in) , 90% (10 min), held...
Ngày tải lên : 08/03/2014, 16:20
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The Sun to the Earth -- and Beyond: A Decadal Research Strategy in Solar and Space Physics potx

The Sun to the Earth -- and Beyond: A Decadal Research Strategy in Solar and Space Physics potx

... Earth and Beyond A Decadal Research Strategy in Solar and Space Physics Solar and Space Physics Survey Committee Committee on Solar and Space Physics Space Studies Board Division on Engineering and ... future directions of U.S. ground- and space- based programs in solar and space physics research. The NRC’s Space Studies Board and its Committ...
Ngày tải lên : 05/03/2014, 11:21
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