... physiologicalpathways, including blood coagulation, haemostasis and the production of anti-phospholipid antibodies (APA).b2GPI inhibits the contact activation of the intrinsicpathway by binding to and neutralizing ... according to the manufacturer’sinstructions. Bovine serum albumin was used as standard to determine the protein concentrations in the liver lysates.Plasmid DNA constructs The 5¢ flanking region of ... protein levels: the promoter region, splicejunctions and the coding region. In our extensive survey of the coding region and the intron–exon boundaries,we have not found causative mutations, other...