... equation where F0is the fluorescence atzero ligand concentration, Fmis the maximum fluorescencechange, T is the total ligand concentration, P is the proteinmonomer concentration, and Kais the ... slow monomer dimer equilibrium. Toconfirm that there is an equilibrium between the two forms, the monomeric (0.3 lMmonomer concentration) and dimeric (1.1 lMmonomer concentration) fractions ... analyzed bynonlinear regression curve fitting using the followingequation, where v is the number of moles of Ca2+boundper mol of monomer, x is the free Ca2+concentration, n is the number of binding...