... Fluorescent analogs of UDP-glucose and their use in characterizing
substrate binding by toxin A from
Clostridium difficile
Sudeep Bhattacharyya, Amy Kerzmann and Andrew L. Feig
Department of ... sugar complexes for use in studying glycosyl-
transferases in general and TcdA in particular. These
substrate analogs are related to the commercially...
... CD53, can have a
signalling role by themselves. Natural ligands of CD53 or
other tetraspan proteins are not yet known. In a way
tetraspanin proteins can be considered as orphan receptors,
and consequently ... immunoprecipitate by the
incorporation of radioactivity in the GST–ATF2 fusion protein used
as substrate. At the top is the assay of the tran sfected HA-JNK activity...
... divided into domains V and
VI. Domain II possesses the active site and is a papain-like
cysteine protease domain, and domain IV contains a
calmodulin-like Ca
2+
-binding domain with multiple
EF-hand ... damage, and neurodegenerative con-
ditions such as Alzheimer’s and Parkinson’s disease [10,11].
The major calpains are l-calpain (calpain 1) and
m-calpain (calpain 2), which...
... between
both assays is the coupling of AR.NTD to GalAD in the
yeast assay, and the absence of a second transactivation
domain linked to AR NTD in the mammalian assay. The
latter assay completely depends ... the
23
FQNLF
27
motif in AR N/C interaction, these amino acids
were substituted by 24/25AA. In both the yeast and
mammalian protein interaction assay, GalAD-
AR.NTD24/2...
... mutagenic primer was 5¢-GGAAATC
ACACCATTATGACCA
AAAACCAGCCCGGGATA
GGC-3¢. The underlined codons code for bF24 and bF57
and were changed to ATA, CCA, AGC and CAA to
introduce Tyr, Trp, Ala and ... acylation by PGA in a similar way as the
acylation by NIPAB. A notable exception was the twofold
increased activity for PGA of the aF14 6Y mutant. It
appeared that this mutant ha...
... sequences, and
of a hydrophobic residue in c-type crystallins.
Contrasting results were obtained in another laboratory,
showing that a recombinant b-crystallin variant is isolated
as a dimer also when ... presence of
spliceosomal introns in eukaryotes, and their absence in
archea and in eubacteria [38–40]. Over recent years, a vast
amount of data has been inter...
... lead concentrations may
be increased in patients with CAD.
Key words: coronary artery disease; trace element; heavy metal; vitamin
INTRODUCTION
Coronary artery disease (CAD) is a leading cause ... patients hav-
ing angiographically demonstrated CAD and 20 pa-
tients having normal coronary arteries attending car-
diology clinic at Yuzuncu Yil University Hospital. The
study was appro...
... contain topics in a text have cer-
tain positional patterns in a paragraph (Lin and
Hovy, 1997), which may apply because reasons
like pros and cons in a review document are most
important ... you are in a train station or a busy
amusement park that is ill-staffed to meet de-
mand!”, “preferential treatment given to large
groups”, and “they don't offer salads...
... Spain
Introduction
Primary hyperoxaluria type I (PHI) is a rare autosomal
recessive disease caused by mutations in the alanine-gly-
oxylate aminotransferase gene (AGXT). Alanine-glyoxy-
late aminotransferase ... favored
over aminobutyrate-pyruvate, b-alanine-pyruvate and
dimethylarginine-pyruvate aminotransferase activities.
In the rat, gluconeogenesis from l-serine takes place
ma...