... 5¢-side of the
processing stem, gave rise to rRNA with the majority
of termini in the mature form, whereas pD3, with a
mutation on the 3¢-side, gave rise rRNA with the
majority of the termini in the ... fractions,
respectively. In particular, there was an increase in the
relative amount of the fully mature terminus, with a
concurrent decrease of th...
... analyzed the
effect of HSP25 on the polymerization of intact actin and
aggregation of heated actin.
Interaction of HSP25 with intact actin
In the first series of experiments we analyzed the effect of
HSP25 ... Effect of heating on the kinetics of polymerization (A) and salt-
induced increase of the light scattering (B) of actin. Actin (15 l
M
)
containing 1...
... signaling, and they strongly affect
interactions of many GlnK proteins with their recep-
tors [17]. The divalent cations Mg
2+
or Mn
2+
were
previously shown to negatively affect the binding of
ATP to ... on the recovery of TnrA fol-
lowing nitrate deprivation, in agreement with the lack
of TnrA degradation in this strain. GS was copurified
with TnrA and the recover...
... 17.6
Fig. 5. Insertion of stefins into DOPC and DOPG monolayers. (A)
Kinetic traces of the insertion into DOPG lipid monolayers at initial
pressure of 5 mNÆm
)1
. The proteins were injected into the sub-
phase ... selective [10].
That the interaction with membranes is involved in
amyloid-induced toxicity is supported by the finding
that cholesterol can modify this interac...
... chondro-
cytes [5]. As YKL-40 is only composed of the sole
Glyco_18 domain, the C-terminal tail of C. gigas CLPs
may not noticeably contribute to the structure and the
function of these proteins. Interestingly, ... together
chitinases and proteins devoid of catalytic activity due
to the substitution of a critical amino acid in the cata-
lytic centre. This latter singul...
... with
BY4743Dcoq2, indicating that they were unlikely to contain
a functioning mitochondrial respiratory chain. Although
the ORFs deleted in these strains could be involved in CoQ
uptake, they ... 2077
binding affinity of CoQ
2
or CoQ
4
for a hydrophobic
pocket in a protein through the loss of isoprenoid
units from the side chain of CoQ
6
. To identify proteins
or pathways...
... moiety [36]. The
presence of such a moiety would possibly account in part for
the protein s observed heterogeneity when analyzed by IEF.
Moreover, the truncation of the protein chain beyond the
site ... 0.15% of total protein) in nearly
equimolar amounts (Fig. 6). Ovisacs containing nonency-
sted embryos contained considerably less of the Artemia
CLP subunits (0.03...
... containing amino acids 98–110 of
the flavivirus E protein) may serve as an internal
fusion peptide, as it is directly involved in interactions
with target membranes during the initial stages of
membrane ... localization in the inner leaflet of the bi-
layer of the second one. In a previous study [14], it has
been suggested that the preferential interaction of t...
... other impor-
tant protein lipid and protein protein interactions there.
Indeed, reconstitution systems using bilayer vesicles yielded
higher rates of activity w ith rabbit liver CYP3A6 and
CYP2B4 ... Molecular Medicine, Berlin, Germany
Human cytochrome P 4501A1 (CYP1A1) is one of the key
enzymes in the bioactivation of environmental pollutants
such as benzo[a]pyrene (B[a]P) and...
... Phosphoryla-
tion of HSL by protein kinase A (PKA) is central to
the molecular control of lipolysis, but other events,
notably phosphorylation of the lipid droplet protein
perilipin, are also of key ... found
to bind tightly to LPL in the vicinity of the active site
and also to inhibit the enzymatic activity [12]. Simi-
larly, we show here that bis-ANS inhibits the...