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Báo cáo khoa học: "THE DESIGN OF THE KERNEL ARCHITECTURE FOR THE EUROTRA* SOFTWARE" pptx

Báo cáo khoa học: Structural recognition of an optimized substrate for the ephrin family of receptor tyrosine kinases pot

Báo cáo khoa học: Structural recognition of an optimized substrate for the ephrin family of receptor tyrosine kinases pot

... aresubtle, the phosphotyrosine at the +2 plays an impor-tant role in reordering the structure of the region of the C-terminal lobe that interacts with these substrates.In the absence of the phosphate ... shows clear density for most of the substrate pep-tide, including main chain atoms for the )4 through to the +4 position, but partial or no density for the sidechains of the N-terminal three ... comprised of residues Arg712 and the regionincluding residues Arg823–Asn830, whereas the OPT-YF peptide does not; therefore, the loss of the interac-tion with Asn830 would be more significant for the OPTYF...
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Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc

Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc

... primersused for PCR amplification. a + c for amplification of the larger IAtranscript; b + c for amplification of the shorter IB transcript. (B)PCR amplification of the two specific transcripts and of the ... identifies for the first time, the presence of alternative promoter usage for the MGP gene and provides clear evidence for differentialexpression of this gene during the very early stages of embryonic ... development.ResultsIdentification of a functional proximal promoter for X. laevisAlignment of the 5¢-flanking region of exon IB from the XlMGP gene with the 5¢-flanking regions of ATG-containing exons of mouse, rat...
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Báo cáo khoa học:

Báo cáo khoa học: "Automatic Induction of Finite State Transducers for Simple Phonological Rules" pptx

... corresponds to the end of an input sample. The output symbols are placed as near the root of the tree as possible while avoiding conflicts in the output of a given arc. An example of the result of this ... have the same behavior if they agree each of the following: • the index i of the output symbol corresponding to the input symbol (determined from the alignment procedure) • the difference of ... to each state of the machine, describing the behavior of that state. For exam- ple, the decision tree for state 2 of the machine in Figure 1 is shown in Figure 8. Note that if the underlying...
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Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... 4452–4463 ª 2010 The Authors Journal compilation ª 2010 FEBS 4453 the a-hydrogen of the substrate, and the other isG374, which forms the binding pocket for the branched-chain acyl moiety of substrate ... the skuphenotype. Because G376V was a novel mutation inIVD, the effect of the mutation was further investi-gated by molecular modelling. Together with the previ-ously reported traits of the ... nucleotidelength of each part is also shown. The sin-gle nucleotide substitution 1127G>T in the sku mutant is represented as a dashed line. The position of the primers used for RT-PCRand that of the...
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Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... CCL13enhances the proliferation of FLS [13,47]. Our groupreported that the proliferation of FLS by MCP-4 ⁄ CCL13 is dependent on activation of the extracellu-lar regulated kinase MAPK. The activation of ... protective against the development of RA [61]. The gene copy number variations of CCL3-like 1(CCL3L1), a nonallelic isoform of CCL3 encoded bydifferent genes and a potent ligand for CCR1 andCCR5, ... primarily affects the small joints of the hands and feet. The inflammatory process is characterized by infiltration of inflammatory cellsinto the joints, leading to proliferation of synoviocytes...
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Tài liệu Báo cáo khoa học: Influence of modulated structural dynamics on the kinetics of a-chymotrypsin catalysis Insights through chemical glycosylation, molecular dynamics and domain motion analysis pptx

Tài liệu Báo cáo khoa học: Influence of modulated structural dynamics on the kinetics of a-chymotrypsin catalysis Insights through chemical glycosylation, molecular dynamics and domain motion analysis pptx

... observed for the glycosylated a-CT conjugates as these residues aredirectly involved in the hydrogen transfer steps of cata-lysis. A decrease in the dynamics of these two loopscould reinforce the ... (nucleophilicity) for the in silico creation of the various glycoconjugates wasdetermined by calculating the electrostatic potentials (EP) for the Ne of the lysine residues of a-CT at pH 9 were the chemical ... within a cutoff distance (rc¼ 6.0 A˚). The dynamics of the resulting network are then defined by the Ni · Nj Kir-chhoff connectivity matrix of interresidue contacts (G)where the off diagonal...
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Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... 3B). The pK2increased as the size of the residue at position 169 increased. The effect of the size of the residue at position 169 may berelated to the solvent accessibility of the side-chain of His210 ... nucleophilicity of the Ser194 hydroxyl oxygen. Accord-ingly, the Asp113–His57 diad is primarily important for the expression of the nucleophilicity of the catalytic Ser194. The side-chain of Asp113 ... [16,17]. The parallel orientation of the imida-zole–indole pair formed between Trp169 and His210 is the first case found in the active sites of serine proteases.Although the role of a proton donor for...
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Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt

Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt

... that for Metand Cys, whereas the kcat of EhMGL2 for TFM was16–22-fold higher than that for these physiologicalsubstrates. The Km of EhMGLs for TFM was 52–470-fold lower than that of the ... molecular ratio of 1 : 1000,and further tested for the Met-degrading activity on the basis of the detection of a-keto acid after the addi-tion of 2 mm Met (Fig. 3). Approximately 85% of EhMGL2 activity ... iso-leucine biosynthesis and the production of S-methyl-cysteine, the putative storage molecule for sulfide ormethyl groups, which is formed by the transfer of the acetyl moiety of O-acetyl-l-serine...
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Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx

Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx

... interactions.To be specific, the dissociation constants of the var-ious phosphorylation states of the target for the kinasewere all set equal to L0, except for the value LN for the fully phosphorylated ... Schematicrepresentation of a phosphorylation–dephosphorylation cycle. (B)Mathematical model for a sequential mechanism of multiple phos-phorylation based on the schema of Fig. 1A. The free form of the n-times ... is of note that the number of different possible sequences to achievefull phosphorylation of the target is 1 for the sequential mechanismand N! for the random mechanism.Kinetic models of multisite...
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Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

... PAGE(Fig. 3) showed that the purified enzymes were of the expected molecular mass of 48 kDa for the full-lengthenzyme and 42 kDa for the truncated enzyme. The yield of recombinant proteins purified ... 30 °C, the oxidation of the substrate DHO with the quinone cosub-strate was coupled to the reduction of the chromogen 2,6-di-chloroindophenol. The Km of DHO was determined byvarying the concentration ... second fragment. The overlappingPCR fragments were then used as templates for PCR withprimers Ca-forlong2 and CaDHODH-rev. For subcloning of the DHODH ORF the restriction sides for BamHI ⁄EcoRI...
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