Báo cáo Y học: Regulation of a1,3galactosyltransferase expression in pig endothelial cells Implications for xenotransplantation doc

... Regulation of a1,3GalT expression in pig endothelial cells (Eur. J. Biochem. 269) 1473 Regulation of a1,3galactosyltransferase expression in pig endothelial cells Implications for xenotransplantation Dominique ... promoter of the long form of b1,4-gala ctosyl-transferase contains 12 Sp1 binding sites, and is active in avariety of cell types [42, 43]. Two putative NF-jB b indingsites have been found in promoter ... 2002 Regulation of a1,3GalT expression in pig endothelial cells (Eur. J. Biochem. 269) 1467sites that could be important for promoter activity in pig cells, such a s GATA- and GC-boxes, AP-1, Inr...

Báo cáo Y học: Inhibition of hyaluronan synthesis in Streptococcus equi FM100 by 4-methylumbelliferone doc

... HA releasedinto the culture medium was measured by the sandwichbinding protein assay using hyaluronan binding protein(HABP) according to the manufacture’s instructions for thehyaluronic acid ... level of the hasoperon containing the hyaluronan synthase gene or theprotein level of hyaluronan synthase. Solubilization of theenzyme from membranes of MU-treated cells and addition of the ... that theinhibition of HA production was not caused by directinhibition of HAS activity.Effect of MU on HAS activity in FM100 cells treatedwith MUThe activity of the HAS in FM100 cells cultured...

Báo cáo Y học: Regulation of glypican-1, syndecan-1 and syndecan-4 mRNAs expression by follicle-stimulating hormone, cAMP increase and calcium inﬂux during rat Sertoli cell development pptx

... glycosylphophatidylinositol (GPI)anchors [1,2]. Although the primary structure of the glypi-cans is only marginally conserved (about 30% identity), thereis a strict conservation of 14 cysteine ... within thecore protein leading to compact conformation but also of HS-attachment consensus sequences at the C-termini of proteins.Syndecans and glypicans are individually expressed in distinct ... by growth factors and cytokine [13,14]. In most cells, levels of syndecan synthesis correlate well withsyndecan mRNA levels, suggesting that the regulation ismainly transcriptional [8].Syndecans...

Tài liệu Báo cáo Y học: Regulation of transcription of the Dnmt1 gene by Sp1 and Sp3 zinc ﬁnger proteins doc

... role in progression of the cellcycle and the differentiation of cells [11,12].The methylation of DNA plays a role in the regulation of gene expression [13,14], genomic imprinting [15] andinactivation ... (Fig. 5A,B). In contrast, the intensity of the shifted band that included GST–Sp1 graduallyincreased. Conversely, the addition of GST–Sp3 resulted in a similar reduction in the binding of Sp1 to ... University of Tokyo, JapanThe Sp family is a family of transcription factors thatbind to cis-elements in the promoter regions of variousgenes. Regulation of transcription by Sp proteins is basedon...

Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufﬁcient to lead to ERK1-2 phosphorylation pot

... glycoproteinVI; PtdIns3K, phosphatidylinositol 3-kinase; PVDF, poly(vinylidenediﬂuoride); PKC, protein kinase C; PP1, (4-amino-4-(4-methylphe-nyl)-7-(t-butyl)pyrazola[3,4-d]pyrimidine); SH2, ... ERK activation induced by thrombinis dependent on SRC kinasesSRC family kinases are necessary for the initial signallingevents induced by GPVI, notably for phosphorylation of the G PVI-associated ... protein con taining the RAS-b inding domain o f RAF. Precipi-tated proteins were resolved by 12.5% SDS/PAGE and analysed byWestern blotting using an anti-RAS Ig (top). Proteins of whole celllysate...

Báo cáo Y học: Regulation of stress-activated protein kinase signaling pathways by protein phosphatases pot

... implicated in the mammalian SAPK systems. REGULATION OF SAPK SIGNALPATHWAYS BY PROTEINPHOSPHATASES IN YEAST CELLS A molecular g enetic analysis of ye ast cells indicated that twodistinct protein phosphatase ... phosphorylation of conserved tyrosine and threonine residues within thecatalytic domain. This phosphorylation is mediated by dualspeciﬁcity protein kinases, members of the MAPK kinase(MKK) family. ... dephosphorylates Hog1 in S.cerevisiae both in vivo and in vitro [15]. REGULATION OF SAPK SIGNALPATHWAYS BY PROTEINPHOSPHATASES IN MAMMALIAN CELLS In mammalian cells, like yeast cells, both...

Báo cáo Y học: Regulation of peptide-chain elongation in mammalian cells pptx

... members of main protein kinasesuperfamily, Ryazanov coined the term Ôa-kinaseÕ for thisunusual group of enzymes. Further discussion of this familymay be found in a recent review by Ryazanov ... translo-cation and in poly(U)-directed polyphenylalanine synthesis[26,27], by preventing it from binding to the ribosome [28].Early data showed that the phosphorylation of eEF2 wasincreased by very low ... phosphorylation by MS6Kmay be involved in their regulation in response to serum in vivo.eEF1A and eEF1B are also substrates for phosphoryla-tion by the classical protein kinase C (PKC) isoforms in...

Báo cáo Y học: Regulation of mammalian translation factors by nutrients pot

... addressed by exploring the effect of rapamycin on the control of overall rates of proteinsynthesis. In cell lines, rapamycin generally exerts only asmall inhibitory effect on the rate of protein synthesis ... (1997) Regulation of proteinkinase B and glycogen synthase kinase 3 by insulin and b-adr-energic agonists in rat epididymal fat cells. Activation of proteinkinase B by wortmannin-sensitive and-insensitive ... data indicate thatinsulin is required for the feeding-induced activation of translation, but that increases in insulin levels may not be,thus suggesting insulin plays a permissive role here.REGULATION...

Báo cáo Y học: Repression of FasL expression by retinoic acid involves a novel mechanism of inhibition of transactivation function of the nuclear factors of activated T-cells pptx

... dephosphorylation ismediated by calcineurin, rephosphorylation is catalyzed bya variety of serine kinases, such as glycogen synthase kinase-3, ERK, p38, casein kinase-2, and c-Jun N-terminal kinase[40–43]. ... [3–5].Thus, the elimination of highly activated T -cells by the Fas/FasL system is critical for the downregulation of immuneresponses, the homeostasis of lymphocytes, and the main-tenance of peripheral ... Repression of FasL expression by retinoic acid involves a novelmechanism of inhibition of transactivation function of the nuclearfactors of activated T -cells Mi-Ock Lee1,*, Hyo-Jin Kang1,*, Young...

Báo cáo khoa học: Regulation of the expression and subcellular localization of the taurine transporter TauT in mouse NIH3T3 ﬁbroblasts doc

... within the initial 20 min following theaddition of 14C-labelled taurine. Using taurine uptakewithin the initial 20 min in NaCl medium containingextracellular taurine (0–50 lM) and ﬁtting ... NF-jB and for tonicityresponse element binding protein (Ton-EBP) [10–12]. Theactivity of p53 is regulated by a series of proteinphosphorylations, acetylations and glycosylations of par-ticular ... of osmo- regulation in t he actions of taurine. Amino Acids 19 , 527–546.5. Miyamoto, Y. , Kanayama, A., Inoue, J.I., Konishi, Y. S. &Shimizu, M. (2003) Taurine is involved in oxidation of...

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