Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... G. Berrin et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides Jean-Guy ... itmight have a role in human aging [6].Finally, a b-glucosidase, termed cytosolic b-glucosidase, is present in the liver, kidney, intestine and spleen of humans. This c ytosolic b-glucosidase ... human liver. Furthermore, we investigated the speci®city of the human CBG with r espect to the glyconeand aglycone moieties, and in particular characterized the ef®ciency of the enzyme in hydrolysing...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
... folding of the mutant. The lowerintensity may arise from partitioning of the protein into an aggregated state. The effect of copper binding on the secondary struc-ture was determined using CD in ... protein. Typically an initial injection of 2 lL copperchelate solution was followed by a further 29 injections of 4 lL of Cu(II) into the protein in the sample cell stirredat 300 r.p.m. Injections ... (cystatin B) is a high affinitycopper binding protein, and (b) that amyloid fibrilformation of this protein is diminished in presence of Cu2+ions. Another interesting finding is thatcopper binding...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... potential therapeutic approach when considering the reported high expression of the ankyrin-repeat oncoprotein (gankyrin) in human hepatocellular carcinoma . Gankyrin binds to the cell-cycle regulator ... gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44]. These factors are involved in ethanol-induced liver damage. Thus, the direct injection of ... the reported ethanol-induced expression and activation of cytokines and chemokines in monocytes and macrophages (including Kupffer cells) [41, 42], and ethanol-induced mucosal injury in the...
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Báo cáo y học: "ene expression analysis of human red blood cells"
... mechanism of globin m-RNA stability in human RBCs. Further experiments are needed to understand the mechanisms and the biological meaning of these findings. But gene expression profiling of human ... expression profiling of human erythrocyte could be an important key for under-standing the machinery of anucleate protein synthesis and its meaning in the pathophysiology of diseases. Acknowledgements ... in human RBCs encoding initiation, activation and regulation of transcription and translation (for instance RNA polymerises I,II,III, zinc/PHD finger- DNA-binding proteins, cysteinyl, lysyl-tRNA...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... of cystatin F is hypothetical. Although most cystatins inhi-biting cathepsin L also inhibit cathepsin S, an inhibitoryactivity of cystatin F against cathepsin S has not yet beenreported. The ... of the cystatin F gene and promoter. The structures of the human cystatin F (CST7)andcystatinC(CST3) genes are illustratedschematically at the top. In the upstream region of the CST7 gene, the ... 88–90 (cystatin C numbering).Recombinant cystatin F expressed in Sf9 insect cells isindeed glycosylated [5].Compared to other cystatins, cystatin F is an unusuallyspecific inhibitor. Among the...
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Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... found in DM43 first threedomains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64.Cytotoxicity was analyzed in vitro using ... effectively neut-ralized the myotoxic and cytotoxic effects of both myotox-ins. Interestingly, DM64 did not inhibit the PLA2activity of myotoxin I nor its intracerebroventricular lethality and its anticoagulant ... sequences. The conserved aromatic residues phenylalanine and tyrosine typical of the Ig-fold are bold in each domain.Fig. 5. Inhibition of in vivo myotoxicity of myotoxins I or II by DM64.Groups of...
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Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu pptx
... Alternatively, the B-ringhydroxylation of naringenin to (2S)-eriodictyol preceeding the 3b-hydroxylationyields the substrate (2R,3R)-dihydroquercetin.Both dihydrokaempferol and dihydroquerce-tin ... Germany) asrequired for the substitution of glycine by alanine andproline, respectively, or of proline by glycine (Table 1). Eachindividual mutation was verified by DNA sequencing using the dideoxynucleotide ... by alanine or proline and the exchange of proline by glycine. Glycine residues are frequently found at the C-terminal ends of a helices providing the conforma-tional flexibility required at these...
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Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
... shock/a-crystallinproteins, and a two or three domain structure is proposedfor these proteins [7,8]. The a-crystallin domain, locatedtoward the C-terminus of the protein monomer, consists of 80–100 ... of colony forming units (cfu) per mL were plotted against the length of heat shock in min. The results shown are the average of three independent experiments. Bacteria containing only pET21(+)did ... variable, leading to the idea thatstructural plasticity elicits low specificity and permitsbinding of different target proteins [10,24,42]. Severalmolecules of denaturing proteins, present in an unstablemolten...
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
... under the control of the GAL10 pro-moter. The induction of the yeast, by galactose containingmedia, results in overexpression of GAL4, in turn inducingan increase of the expression of the OR ... Controls for the immunocytochemicalreaction were carried out by replacing either the primary or the secondary antibody by the incubation buffer in the reaction sequence. The sections were finally viewed ... to the difficulty in maintainingolfactory neurons in primary culture, the inconsistency of viral-mediated gene transfer, and the cost if it was tobe applied to a large number of ORs. The aim of...
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Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... obtain a recombinant proteincontaining the C-myc epitope followed by six histidines at the C-terminus. A plasmid containing the wild type version of CCR5 was constructed by introducing the codingsequence ... plasmid. The resulting XhoI/ApaI fragment was cloned into the corres-ponding sites in the polylinker region of the pcDNA3.1-myc-His expression vector (Invitrogen, Groningen, the Netherlands) in order ... glycosaminoglycans [37].As the myc-his tag of the protein was present at the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction bythe...
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