... Dependence of the rate of FMN synthesis on Rf concentra-tions. (C,D) Time courses of FAD synthesis and Rf appearance after addition of either1 lM or 50 lM FMN. Values are the mean of three replicates ... S0.5=nÖKm.To fit the experimental data and to obtain estimates of the kinetic parameters, use was made of the grafit soft-ware (Version 3.00, 1992, by R. J. Leatherbarrow, Eritha-cus Software, Horley, ... at 37 °Cwith ATP (1 mm) and either Rf or FMN in the pres-ence of MgCl2(5 mm) (Fig. 5). The amounts of FAD,FMN and Rf in the neutralized perchloric acidextracts of the suspension were measured...