... constructed a pro-tyrosinase mutantcarrying an alanine at this position, F45 3A. Curi-ously, an increase in protein expression was obtainedfor this mutant tyrosinase similar to that obtainedwhen ... tyrosinase constructs without thepredicted N-terminal signal peptide (amino acids1–36), we retained amino acid 36, an alanine, ratherthan using amino acid 37, a lysine, because it is known that, ... pre-pro-tyrosinase (Fig. S1) can be dividedapproximately into three domains: a twin argininetranslocase (TAT) signal peptide, a core domain con-taining the two copper-binding motifs and a C-termi-nal...