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Báo cáo khoa học: Supramolecular calsequestrin complex Protein–protein interactions in chronic low-frequency stimulated muscle, postnatal development and ageing pot

Báo cáo khoa học: Supramolecular calsequestrin complex Protein–protein interactions in chronic low-frequency stimulated muscle, postnatal development and ageing pot

Báo cáo khoa học: Supramolecular calsequestrin complex Protein–protein interactions in chronic low-frequency stimulated muscle, postnatal development and ageing pot

... calsequestrin complex Protein–protein interactions in chronic low-frequency stimulated muscle, postnatal development and ageing Louise Glover1, Sandra Quinn1, Michelle Ryan1, Dirk Pette2 and Kay Ohlendieck31Department ... process. The increase in calsequestrin and decrease in junctin expression during postnatal development resulted in similar changes in theintensity of binding of the calsequestrin conjugate to ... TRI and the CSQ-binding elementJUN, show decreased protein–protein interactions duringfiber type shifting and the ageing process. Reduced proteincoupling between the major elements regulating...
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Báo cáo khoa học: Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions doc

Báo cáo khoa học: Light-harvesting complex II protein CP29 binds to photosystem I of Chlamydomonas reinhardtii under State 2 conditions doc

... observed in theS2 LHCI–PSI supercomplex in the vicinity of PsaH isindeed phosphorylated CP29, modelled in blue in Fig. 5 according to the X-ray structure of the LHCIIprotein [30]. In order ... thylakoids and LHCI–PSI obtained from State 1 and State 2 C. reinhardtii cells. (A) Proteinprofiles of thylakoids (Thy) and LHCI–PSI (F3) complexes obtained from psbD-His State 1- (S1) and State ... densityindicative of binding of LHCII in this region. Indeed,to date, there is no direct structural evidence of howphosphorylated LHCII binds to PSI, although recentcross-linking and antisense...
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Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... accumulation and consumption in Paracoccus pantotro-phus strains. Starter cultures were grownaerobically in LB with shaking before inocu-lation of mineral salt medium containing20 mM nitrate in a ... to that in Asp141 of Met8P.The idea of NirF being a dehydrogenase is appealingbecause of the presence of a putative nucleotide-bind-ing motif in the N-terminal of the protein sequence and also ... NirF(concentrations of heme and protein were calculatedby using the extinction coefficient mentioned in theexperimental section and the standard Bradford assay,respectively). To test whether the binding of d1hemeto...
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Tài liệu Báo cáo khoa học: The PA-TM-RING protein RING finger protein 13 is an endosomal integral membrane E3 ubiquitin ligase whose RING finger domain is released to the cytoplasm by proteolysis ppt

Tài liệu Báo cáo khoa học: The PA-TM-RING protein RING finger protein 13 is an endosomal integral membrane E3 ubiquitin ligase whose RING finger domain is released to the cytoplasm by proteolysis ppt

... 2009)doi:10.1111/j.1742-4658.2009.06913.xPA-TM-RING proteins have an N-terminal protease-associated domain, astructure found in numerous proteases and implicated in protein binding, and C-terminal RING finger and PEST domains. Homologous ... zinc ions. The RING subfamily isthought to function exclusively in protein–protein interactions rather than protein–nucleic acid interac-tions [8]. Many RING finger proteins are E3 ubiquitinligases ... ubiquitination system functions in avariety of cellular processes, including protein degra-dation and protein trafficking.PA-TM-RING proteins that combine these twodomains have been identified in...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc

... proteins, 73% are proteins involved in mRNA stability, but there are also proteins involved in other cellular processes, such as actin. Further stud-ies characterizing proteins interacting with ... E185, D226, D244) in PINdomains involved in binding Mg2+ions are conserved in all three sequences. Other conserved residues areR263, V139 and N144. Determination of the MCPIPdomain architecture ... are exerted by the PIN domain, asshown by experiments with mutated MCPIP (either lack-ing the PIN domain, or with two conservative aminoacid mutations within PIN domain). Interaction ofMCPIP...
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Tài liệu Báo cáo khoa học: The Mycobacterium tuberculosis membrane protein Rv2560 ) biochemical and functional studies pdf

Tài liệu Báo cáo khoa học: The Mycobacterium tuberculosis membrane protein Rv2560 ) biochemical and functional studies pdf

... protein encoding gene and its transcripts in the M. tuberculosis complex and clinical isolate strains,as well as the characterization of the high-activitybinding peptide (HABP) involved in the ... proteins that bind to biotinylated cellfractions, including the already characterized adhesionheparin-binding haemagglutinin [27].A B Fig. 5. Saturation curves and critical residues of cell binding. ... show any binding toHepG2 cells or erythrocytes (data not shown).Invasion inhibition assay using HABPsA549 and U937 cell lines were preincubated in thepresence of HABP 11024 in two independent...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx

Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx

... Ne-carboxymethyl-lysines and Ne-carboxy-ethyl-lysines and pentosidine) being known to accumu-late with age on tissue proteins, and at an increasedrate in LDL and atherosclerotic lesions in peoplewith ... (1994) Binding and modification ofproteins by methylglyoxal under physiologicalconditions. A kinetic and mechanistic study withNalpha-acetylarginine, Nalpha-acetylcysteine, and Nalpha-acetyllysine, ... reactive amine (e.g. Lys and Argside chains, N-terminus [11–13]) or thiol (Cys) groupson proteins [14], such as those of the single proteinmolecule of LDL, apolipoprotein B-100 (apoB). Theinitial...
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Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc

... to typeII ribosome-inactivating proteins, ricin and abrin.cDNA cloning and molecular modeling study. J BiolChem 271, 1480–1485.41 Wu YH, Shih SF & Lin JY (2004) Ricin triggers apop-totic ... HBS, heparin-binding site; RBC, red blood cell; VVA,volvatoxin A; VVA1, volvatoxin A1; VVA2, volvatoxin A2; VVA1-CTD, volvatoxin A1 C-terminal domain (198–391 residues); VVA1-NTD,volvatoxin A1 ... produced by both prokaryotes and eukaryotes, and include well-known toxins such asdiphtheria and anthrax toxins, as well as the less well-known a-hemolysin and equinatoxin II [11–16]. Animportant...
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Tài liệu Báo cáo khoa học: Mg2+-modulated KMnO4 reactivity of thymines in the open transcription complex reflects variation in the negative electrostatic potential along the separated DNA strands ppt

Tài liệu Báo cáo khoa học: Mg2+-modulated KMnO4 reactivity of thymines in the open transcription complex reflects variation in the negative electrostatic potential along the separated DNA strands ppt

... bandscorresponding to all thymines in the )11 … +2 pro-moter region came up clearly in the footprintsobtained even at 13 °C and the lowest oxidant doseapplied and in the absence of MgCl2. In the ... (A) and template (B) DNAstrands; doses of KMnO4 (in M · s) applied at 37 °C are indicated above lanes 2–11. Minus and plus signs indicate the absence and pres-ence of 10 mM MgCl2 in footprinting ... footprints were obtained with the use of aMolecular Dynamics Phosphorimager. Integrated intensitiesof bands (or groups of bands) and their intensity profilesalong gel lanes were obtained with...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf

Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf

... gene encoding the hybrid protein ofthe GAL4p activation domain and NOP53p. YCpGAL-A–NOP53 was constructed by inserting the BamHI ⁄ SalINOP53-containing fragment obtained from pBTM-NOP53into Ycp33GALl-A ... was obtained by inserting the fragment (Bam-HI ⁄ Sal I) containing NOP53 sequence and the fragment(EcoRI ⁄ BamHI) containing GAL1-HIS sequence into thepRS313 vector digested with EcoRI and SalI. ... protein containingthe lexA DNA binding domain and the full-length NOP53ORF. Transformation of YDG146 was performed withplasmid pGAD-NOP17 containing NOP17 ORF fused tothe GAL4 activation domain....
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