... Schistosoma mansoni egg glycolipids (Eur. J. Biochem. 269) 493
Characterization of glycosphingolipids from
Schistosoma mansoni
eggs carrying Fuc(a1±3)GalNAc-, GalNAc(b1±4)[Fuc(a1±3)]GlcNAc-
and Gal(b1±4)[Fuc(a1±3)]GlcNAc- ... University of Giessen, Germany;
2
School of Biological Sciences, University of Wales, Bangor, Wales, UK
The carbohydrate moieties of glycosphi...
... 10
6
mL
)1
. Next, 0.5 mL of
propidium iodide staining solution (10 mg of propidium
iodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of
NaCl ⁄ P
i
) was added to 0.5 mL of cell suspension. The ... were
analysed in terms of production ⁄ consumption of TG.
In mammalian cells, TG may be deleted to meet the
needs of cell metabolism, either as a source of energy
or as a res...
... addi-
tional C -terminal sequence of about 90 amino acids of
unknown function. The high degree of identity of the
Cg-Clp1 and Cg-Clp2 Glyco_18 domains (84% iden-
tity) argues for a conservation of the ... an additional 90 amino acid C -terminal
sequence of unknown function (Fig. 1). Hence, the
overall structure of Cg-Clp2 is similar to that of
Cg-Clp1.
Expression of Cg-Clp...
... followed by mixture with
6 lL of Lipofectamine Plus reagent at room temperature
for 10 min. Four microliters of Lipofectamine Plus reagent
were diluted in 100 lL of Opti-MEM I-reduced serum med-
ium. ... Department of Legal Medicine and Medical Genetics, Gunma University, Japan
2 Division of Medical Genetics and Biochemistry, University of Fukui, Japan
3 Division of Legal Medici...
... the mechanism of action of PPI1.
The analysis of the pH dependence of PPI1-
induced activation of the H
+
-ATPase showed that
stimulation decreases dramatically with the increase
of pH above pH ... with
PPI1
588
His
6
.
The analysis of the effect of PPI1
588
His
6
on the
dependence of PM H
+
-ATPase activity on the concen-
tration of MgATP (Fig. 3) showed that stimulation...
... from
Tritirachium album Limber. Characterization of the
chromosomal gene and expression of the cDNA in
Escherichia coli. Eur J Biochem 179, 185–194.
Characterization of a Serratia proteinase K-like ... Willassen
1,2
1 Department of Molecular Biotechnology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, Norway
2 The Norwegian Structural Biology Centr...
... of all of these genes remains to be clarified.
Expression and purification of Sno1p and Snz1p
In light of the similarity in the amino acid sequence of
Sno1p to that of the glutaminase subunit of ... with a
library of yeast chromosomal DNA. It was found that 4.6
and 5.1 kb overlapping fragments of chromosome XIII
carrying SNO1 and SNZ1 were capable of complementing
the defe...
... differences
between the TP isoforms in terms of their mechanisms
of signaling [18,19], modes of regulation⁄ desensitiza-
tion [20–23] and patterns of expression [24], this repre-
sents a question of potentially ... or
hypertrophic growth of VSM [5,6], inhibition of angio-
genesis ⁄ neo-vascularization [7] and apoptosis of
CD4 ⁄ CD8
++⁄ –
thymocytes [8]. Alterations in the leve...
... data.
Data analyses
Data were analysed by means of sigmaplot 2001 (SPSS
Science Software Gmbh, Erkrath, Germany). Data for the
characterization of the kinetics of electrogenic BSP uptake
fitted the Michaelis–Menten ... process.
litre/osmol
01234
nmoles of BSP disappeared
0,0
0,4
0,8
1,2
1,6
Fig. 2. The dependence of valinomycin-induced disappearance of
BSP on the osmolarity of...
... of ICAM
transfectants to 0.4 lg of I domain GST
captured with 0.2 lg of coated anti-GST Ab.
Controls include wells with captured GST and
the binding of wild-type L cells. Background
binding of ... Inhibition of adhesion of ICAM trans-
fectants to purified CD11b I domain GST
fusion protein. The effects of anti-ICAM and
anti-I domain mAbs on adhesion of ICAM
transfectants to 0.4...