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Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot

Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot

Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot

... study of uptake of Yb3+ by human Tf (hTf) by UV-vis and NMR spectroscopy, and inductively coupled plasma atomic emission spectro-metry (ICP-AES). The binding of Yb-bound Tf to human erythroleukemia ... filter.RESULTSBinding of Yb3+ to apo-hTfThe thermodynamics of binding of Yb3+ to apoTf.Binding of Yb3+ to apo-Tf was investigated by addition of aliquots of Yb3+ to a solution of apo-TF in 100 ... Complexation of ytterbium to human transferrin and its uptake by K562 cells Xiu-lian Du1, Tian-lan Zhang1, Lan Yuan1, Yong-yuan Zhao1, Rong-chang Li1, Kui Wang1, Siu Cheong Yan2,Li...
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Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf

Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf

... presence of acetylcholinesterase and butyrylcholinesterase mRNAs in kidneysupports their synthesis in the organ itself, and the prevalence of glycosyl-phosphatidylinositol-anchored acetylcholinesterase ... origin of the butyrylcholinesterase activityassayed in kidney. This proposal is in agreementwith the cytochemical staining of acetylcholinesterasein the capsule of Bowman [41] and of acetylcholines-terase ... pRCC (Table 1) may indeedrepresent a side effect of the transformed cell pheno-type, but the binding of the cytotoxic cisplatin to acetylcholinesterase [53] and probably to butyrylcho-linesterase...
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Báo cáo khoa học: Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate potx

Báo cáo khoa học: Oligosaccharide synthesis in Fibrobacter succinogenes S85 and its modulation by the substrate potx

... product of cellulolysis), and with a mixture of glucose and cellobiose. Cell extracts and extracellu-lar media were analysed by 2D NMR spectroscopy and by TLC to identify and quantify the oligosaccha-rides ... were separated by centrifugation and analysed, in parallel, by 1H NMR spectroscopy and by TLC. Consumption of the substrates and pro-duction of the final metabolites (succinate and acetate)were ... previously [10] by using amixture of glucose, cellobiose or maltose, maltotriose and maltotetraose, or phosphorylated sugars (3 gÆL)1; Sigma) asa standard. Samples were spotted onto thin-layer...
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Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot

Báo cáo Y học: Regulation of RAS in human platelets Evidence that activation of RAS is not sufficient to lead to ERK1-2 phosphorylation pot

... proteins were resolved by 12.5% SDS/PAGE and analysed by Western blotting using ananti-RAS Ig (top). Proteins o f whole cell lysate were resolved by 12.5% SDS/PAGE and analysed by Western blotting ... be due to the a bility of TPO to activate the e arly events of the R AS signalling pathway.However, a recent study reported that inhibitors of PtdIns3K abolished potentiation of ERK by TPO inresponse ... secondaryresponses, aggregation mediated by t he integrin GPIIb-IIIawas inhibited by EGTA and the activation m ediated by thromboxane A2 and ADP were blocked by indomethacin and apyrase, respectively....
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Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx

Báo cáo Y học: Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli pptx

... by nickel-chelate chromatography to a specific activity of 1.06 UÆmg)1protein at 2 5 °C and with a yield of 98%. Thecharacterization of the purified enzyme showed that GO is ahomotetramer of ... step by metal-chelate chromatography,producing a stable holoenzyme with a high specific activity and a 98% yield quickly and simply. Its properties(aggregation state, absorbance spectrum and ... (N-methylglycine) to form glycine and formalde-hyde. Similarly, DAAO catalyses the oxidative d eamination of neutral and (with a lower efficiency) basicD-amino acids to give the corresponding a-keto acids and...
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Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx

Tài liệu Báo cáo khoa học: Inhibition of pneumococcal choline-binding proteins and cell growth by esters of bicyclic amines pptx

... corresponding to the binding to ABFig. 1. Spectroscopic analysis of ligand binding to C-LytA. (A) Con-formational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and ... order to establish the types of compound to be examined, and to reduce the num-ber of an otherwise vast set of candidates, we tookinto account: (a) commercial availability; (b) watersolubility ... viability and liberation of viru-lence factors. Inhibition of autolysis by excess cho-line might, in the first instance, seem to be of therapeutic interest. However, the amount of ligandneeded, and...
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Báo cáo y học:

Báo cáo y học: "Use of the measure your medical outcome profile (MYMOP2) and W-BQ12 (Well-Being) outcomes measures to evaluate chiropractic treatment: an observational study"

... easily incorporated intothe practice setting because of its brevity. A comparativestudy of MYMOP and the SF-36 has been conducted [1].MYMOP concurrent validity was supported by its ability to ... words.Symptom 2 S2 Optional and is second symptom which is part of the same problem as symptom 1Activity A An activity of daily living of importance to thepatient in which Symptoms 1 and 2 interferewith. ... two symptoms (physical orMYMOP. Measure Yourself Medical Outcome Profile * MYMOP2 * Full name Date of birth Address and postcode Today’s date Practitioner seen Choose one or two symptoms...
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 Báo cáo y học:

Báo cáo y học: "Optimization of 5-fluorouracil solid-lipid nanoparticles: a preliminary study to treat colon cancer"

... delivery of a high concentration of active agents in the colon to improve pharmacotherapy and reduce its potential systemic toxicity and side effects [3]. The early detection, diagnosis, and ... esters of hydrogenated fatty acids. Hydro-genated cottonseed oil (Lubritab™ or Sterotex™), hydrogenated palm oil (Dynasan™ P60 or Softisan™ 154), hydrogenated castor oil (Cutina™ HR), and hy-drogenated ... (Wtotal 5-FU) and the free amount presented in the aqueous phase of supernatant (Wfre e 5-FU) by the total amount used of 5-FU. The %DL w a s obtained by dividing that difference by the total...
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Báo cáo y học:

Báo cáo y học: "Influence of Cyclodextrin Complexation with NSAIDs on NSAID/Cold Stress-Induced Gastric Ulceration in Rats"

... mechanism of action of all NSAIDs is the inhibition of the biosynthesis of prostaglandins by blocking the activity (selectively and/ or non-selectively) of two isoforms of cyclooxygenase enzyme ... aim of this work was to study the ability of β-cyclodextrin (β-CD) or hydroxypropyl β-cyclodextrin (HP-β-CD) to ameliorate the induction of gastric ulcers by a nonsteroidal anti-inflammatory ... anti-inflammatory drugs. Am J Med. 1998; 104: 25-85. 8. Simon LS, Lanza FL, Lipsky PE, et al. Preliminary study of the safety and efficacy of SC-58635, a novel cyclooxygenase 2 inhi-bitor: efficacy and...
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Báo cáo y học:

Báo cáo y học: "A Practical Approach to Management of Chronic Hepatitis B"

... treatment [16]. 4. Indications of HBV Treatment Historically, levels of HBV DNA and ALT, and histological activity of liver biopsy have been used as the three main factors to determine if a patient ... On-treatment Monitoring and Duration of Therapy Patients who receive HBV treatment should be monitored regularly. The interval of follow-up visits may vary depending on regimen and duration of the HBV ... suppression of HBV DNA, normalization of transaminases, and improved histology. The overall response to one year of ADV treatment as determined by HBeAg seroconversion is approximately 12% (vs....
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