Báo cáo Y học: Double-stranded RNA-activated protein kinase interacts with apoptosis signal-regulating kinase 1 Implications for apoptosis signaling pathways docx
... and characterize
putative protein networks involved in functional pathways
[10 ,11 ].
A different but complementary approach for understand-
ing which proteins functionally interact is to develop ... from
that of a typical protein surface [17 ,18 ]. Similarly, no residue
preference for the interacting surfaces has been reported,
although a recent study carried out on 6 21 protein...
... specifically
interact with members of the 14 .3.3 protein family [37]. The
14 .3.3 proteins are located in the cytosol where they regulate
various aspects o f cell p hysiology. The 14 .3.3 proteins h ave
also been ... , 12 9 13 4.
13 .Hinz,W.,Gruninger,S.,DePover,A.&Chiesi,M. (19 99)
Properties of the human long and short isoforms of the uncoupling
protein- 3 expressed in yeast c...
... recognized by U1/U2
containing spliceosomes [10 ]. In human, dog and mouse
the NICN1 gene is immediately followed by the AMT gene
for aminomethyltransferase, an enzyme of glycine meta-
bolism [4 ,11 ]. ... 50 mgÆmL
)1
streptomycin (denoted met-free medium). Cells were solu-
bilized with 1 mLÆdish
)1
of cold lysis buffer essentially as
described before [8]. The lysates were incubated...
... I.
Enzymes: DNA topoisomerase I (EC 5.99 .1. 2).
(Received 18 February 2002, revised 20 May 2002,
accepted 31 May 2002)
Eur. J. Biochem. 269, 3504–3 510 (2002) Ó FEBS 2002 doi :10 .10 46/j .14 32 -10 33.2002.03037.x
scleroderma ... antibody to human topo I (TopoGEN).
Relaxing activity of the enzyme was 6 .1 · 10
6
±2.2· 10
6
(n ¼ 4) units per mg protein. Htopo I was also active as a...
... was only slightly lower
than that of EGFP itself [2 .1 ± 0 .1, 1. 7 ± 0 .1, and
1. 8 ± 0 .1 (· 10
3
arbitrary units) for EGFP, EGFP–M1,
and EGFP–M2, respectively], indicating that the EGFP
moiety of ... EGFP-M1 EGFP-M2
AS-ODN
% of EGFP
control
% of EGFP
control
% of EGFP-M1
control
% of EGFP
control
% of EGFP-M2
control
– 10 0 ± 6 340 ± 16 10 0 ± 6 19 0 ± 11 10 0 ± 7
AS-5 93 ± 7...
... with
aF-N without competitors; and with competitors, lanes 4, 5 and 6, with
C8; lanes 7, 8 and 9, with C5; lanes 10 , 11 and 12 , with C5Pc-2; lanes
13 , 14 and 15 , with C5gg; lanes 16 , 17 and 18 , ... extracts, 1 · 10
7
NIH3T3 cells were
harvested and washed with NaCl/P
i
, lysed with a 5 cell-
pack vol. of lysis buffer consisting of 50 m
M
Tris/HCl
(pH 8.0),...