Báo cáo Y học: Membrane embedded location of Na+ or H+ binding sites on the rotor ring of F1F0 ATP synthases ppt
Báo cáo Y học: Membrane embedded location of Na+ or H+ binding sites on the rotor ring of F1F0 ATP synthases ppt
... intrinsic rotor channels. The common membrane embedded location of the binding site of ATP synthases suggest a commonmechanism for ion transfer across the membrane. Keywords: coupling ion binding ... with cGlu65 of the ATP synthase of I. tartaricus or cAsp61 of the ATP synthase of E. coli and is therefore suitable for fluorescenceinvestigations.Reconstitution of the E. coli ATP synthaseinto ... Localization of ion binding sites in ATP synthases (Eur. J. Biochem. 269) 5583quenching the accessibility of these sites and their location within the membrane. The results of Fig. 2 show the inactivation...
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Báo cáo y học: "Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain"
... behavioral hypersensitivity (3-5). A cur-rent theory for the etiology of neuropathic pain in-volves CNS immune activation with cytokine pro-duction inducing the expression of final common pain ... administered intrathecally once daily for 7 days, starting from 1 day before CCI surgery. Evaluation of tactile allodynia and thermal hyperalgesia The paw withdrawal latency (PWL) to radiant ... the activation of NF-κB p65 and production of proinflammatory cytokines (e.g., TNF-α and IL-1β). Conclusions/Significance: These findings suggest that suppression of TLR4 mediated by intrathecally...
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Tài liệu Báo cáo Y học: Granule-bound starch synthase I A major enzyme involved in the biogenesis of B-crystallites in starch granules ppt
... pre-existing amorphous amylopectin into the formation of B-type crystals. Another mechanism would consist of crystallization of newly synthesized molecules into B-typematerial. This could involve only de ... type of crystals synthesizedunder these conditions show that GBSSI induces the formation of B-type crystals either in close associationwith pre-existing amorphous amylopectin or by crystalli-zation ... amylosesynthesis on granule organization and more specifically on starch crystallinity. STA2 gene disruptions do not yield anymeasurable glucose residue incorporation under theseconditions and the...
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Báo cáo Y học: A chimeric scorpion a-toxin displays de novo electrophysiological properties similar to those of a-like toxins docx
... quinquestriatus hebraeus scorpions [15].Classically, binding of scorpion a-toxins to the receptorsite 3 on the extracellular surface of sodium channelsinduces prolongation of action potentials due ... show any effect neither on action potential or sodium current decay as compared to BotXIV (data notshown). These data confirm the absence of insect toxicitynoticed previously. On the other hand, ... (B)application. Note the progressive evolution of the action potentialdurations under BotXIV (A) and the axonal membrane depolarizationunder M8-10 associated to a slight prolongation of AP duration...
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Báo cáo Y học: Amino acids 3–13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf
... function of AR.NTD. Alternativeexplanations might be induction of a more favorableNTD conformation or stabilization of the in vivo N/Cinteraction, which are not reflected in the pull-down assaysand ... Together with coactivators, generaltranscription factors and RNA polymerase II, they form astable transcription initiation complex [1–4].Upon ligand binding, the LBD acquires a conformationthat ... coactivators and enables the binding of corepressors[3,5].P160 coactivators not only bind to the LBD, but also to the NTD [6,7]. This interaction is independent of the NRboxes. As shown for the...
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Báo cáo Y học: Guanosine diphosphate-4-keto-6-deoxy-D-mannose reductase in the pathway for the synthesis of GDP-6-deoxy-D-talose in Actinobacillus actinomycetemcomitans potx
... and the stereoselectivity of the reduction deter-mines the direction of synthesis of these two 6-deoxyhex-oses. However, neither the gene encoding the biosynthesis of GDP-6-deoxy-D-talose nor ... respectively) to the proteins enco-ded by ORFs in the clusters responsible for synthesizing the SPAs in other serotypes of A. actinomycetemcomitans. The biosynthetic pathway for GDP-6-deoxy-D-talose,Fig. ... 48.0% [43]. The GC content of the region essential for the biosynthesis of SPA in the otherserotype strains (b–e) of A. actinomycetemcomitans is alsolower than the average GC content of these genes...
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Báo cáo Y học: Irregular spiking in free calcium concentration in single, human platelets Regulation by modulation of the inositol trisphosphate receptors ppt
... probability of the receptor channels are the luminal Ca2+concentration in the endoplasmic reticulum[9,10], modulation or oxidation of the receptor sulfhydrylgroups [11–14], and phosphorylation b y ... Heemskerk11Departments of Biochemistry and Human Biology, University of Maastricht, the Netherlands;2Department of Medical Physiology,University Medical Centre Utrecht, the NetherlandsFluorescence ratio ... mostly type 1and type 2 receptors in addition to some type 3 receptors[23–26]. In p latelet membrane preparations it is shown that the InsP3receptors are susceptible to sulfhydryl modifica-tion...
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Báo cáo Y học: Cytochrome c from a thermophilic bacterium has provided insights into the mechanisms of protein maturation, folding, and stability potx
... stability of HT c-552, and the corresponding regions in the wild-type PA c-551 and itsquintuple mutant. The mutated residues in the quintuple mutant, and the corresponding ones of the wild-type ... information on the mech-anism underlying protein stability.BIOGENESIS OF BACTERIALCYTOCHROMES cTo confirm experimentally the amino-acid residues respon-sible for the stability indicated by ... production level The yields of heterologously expressed cytochromes c maydepend on the copy numbers of the plasmids used. Inaddition, coexpression with ccm genes is effective for higherlevels of...
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Báo cáo y học: "Iraqi health system in kurdistan region: medical professionals’ perspectives on challenges and priorities for improvement"
... professionals’ rating of the quality of servicesand availability of resources in the health institutions,their view on different aspects of the health system, andfinally identifying the priority ... profes-sionals in clinical practice. The questionnaire was directly dispatched to the selected sample with a brief description of the purpose of the study, clarification of the type of questions ... rating the health services while they are the one who deliversuch services. The primary focus of the study was the public sector of the health system. Even though the role of private sector in...
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Báo cáo y học: "Parvovirus B19 Genotype Specific Amino Acid Substitution in NS1 Reduces the Protein’s Cytotoxicity in Culture"
... substitution of isoleucine 181 for methionine, as occurs in B19 genotype II, results in the reduction of B19 NS1-induced cytotoxicity of liver cells. Our results support the hypothesis that in the ... po-tential orientation of the side chain and hence a change in the secondary structure of that region in the protein. Since the I181M substitution did not oblite-rate B19 NS1 cytotoxicity, but only ... NS1-induced cytotoxicity (34, 35). Further confirmation for the reduction of apop-tosis in the PathI transduced population of HepG2 cells was obtained using cell cycle analysis of HepG2 cells...
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