Báo cáo khoa học: Unique features of the hemoglobin system of the Antarctic notothenioid ﬁsh Gobionotothen gibberifrons ppt

... Unique features of the hemoglobin system of the Antarctic notothenioid fish Gobionotothen gibberifrons Panagiotis Marinakis, Maurizio Tamburrini, Vito Carratore and Guido di PriscoInstitute of ... Naples, ItalyThehemolysateoftheAntarcticteleostGobionotothen gibberifrons (family Nototheniidae) contains two hemoglo-bins (Hb 1 and Hb 2). The concentration of Hb 2 (15–20% of the total hemoglobin ... environmental conditions which the fish may have toface. The unique features of the oxygen-transport system of this species are reflected in the phylogeny of the hemoglobin amino-acid sequences,...

Tài liệu Báo cáo khoa học: Unique features of recombinant heme oxygenase of Drosophila melanogaster compared with those of other heme oxygenases studied docx

... side of the heme. In DmHO,Thr35 corresponding to Thr21 of HO-1 is conserved, but the other two amino acid residues are not. The crystalstructure of HO-1 also shows that the backbone atoms of the ... Ascorbate was omitted from the control system. Reduction was initiated by the addition of ascorbate. Other conditions were the same as those for the NADPH/DmDCPR system. Reaction of hemin bound to DmDHO ... glucose at 37 °Covernight.Then 200 lL of the preculture was added to 500 mL of the same medium for incubation at 37 °C. After the A600 of the culture reached about 1.0, the incubation wascontinued...

Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt

... iscapable of reducing the level of fructose 2,6-P2inde-pendent of the phosphorylation state of iPFK-2.Reducing the level of fructose 2,6-P2and thus the activity of PFK-1 improves the supply of ... the activity of the enzyme by x%results in a signiﬁcant change of the pathway ﬂux byat least 0.5x% (whether 0.5x% or higher is a matter of convention). The way that the change of enzyme activ-ity ... Ca2+concentration leads todetachment of PFK from the cytoskeleton and thusdiminishes the provision of local ATP in the vicinity of the cytoskeleton [71]. The expression of PFK in tumorcells can be...

Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

... region.Effect of mutations in the C-terminal region of the RmInt1 IEP on RNA splicing in vivoWe constructed a series of mutants to identify the functional features of the C-terminal region of the RmInt1 ... resi-due at the N-terminus of a-helix a4, the domain Xcontains the conserved amino acid residues HKXRA(RmInt1 residues 388–392) carrying a stretch of basicamino acids. Some of the residues of the ... [10,11].Biochemical analyses of LtrA mutants (the IEP of the Ll.ltrB intron of Lactococcus lactis) have suggestedthat the N-terminus of the RT domain is required forprotein interactions with the high-afﬁnity...

Báo cáo khoa học: Mercury(II) binding to metallothioneins Variables governing the formation and structural features of the mammalian Hg-MT species pptx

... that the stoichiometries of the Hg-bMT species becomedependent on t and X after t he forma tion of Hg7-bMT.Comparison of the three sets of CD data indicates that the degree of chirality of the ... provide information on the binding of Hg(II) to the corresponding apo-MT form [23]. In a ddition, compar-ison of the two sets of data gives an indication of the role of Zn(II) in the Hg(II)-containing ... as evidenced by the comparison of the CDspectra of isostoichiometric species obtained under differentconditions. The contribution of the counter-ion to the 3Dstructure of the Hg-MT aggregates...

Báo cáo khoa học: " Medication errors: a prospective cohort study of hand-written and computerised physician order entry in the intensive care unit"

... (where the patient received the drug). The intercepted errors were scored separately on the basis of their possible impact on the patient, if the prescriptionhad been administered as prescribed. The ... blindedto the prescribing system; this could have introduced the potential for bias in the results and is a limitation of the study.Medical errors are among the leading causes of death in the United ... once in the proportion of error analysis. This explains why the total of hand-written prescribing (HWP) error types stated here is in excess of the total number of errors stated in the results...

Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx

... characterization of these compounds isgrowing and may result in the synthesis of speciﬁcinhibitors based on the structure of the pathogensiderophore [16].A promising approach for the isolation of secondarymetabolites, ... derivatization-basedelucidation of the overall stereochemistry. Further-more, the functional properties of erythrochelin actingas an iron-chelating compound were investigated. On the basis of the analysis of the S. ... composed of a hOrn and a haOrn moiety.Furthermore, a long-range1H–13C correlation wasdetected between the carbonyl carbon of the serine and the d-CH2 of the hOrn, which constitutes the DKP.Therefore,...

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... [8,9]. One of the interesting features of the plant PSs is the presence of a conserved 24 residue insertion in the sequence [9].This sequence of amino acids, which is missing in the sequence of bacterial ... pantothe-nate from low amounts of pantoate [9]. Here, wereport the results of the structural studies of the panto-ate-bound form of the N-terminal domain of E. coliPS (nPS). The structure and substrate-binding ... [13].Details of the structural basis for the biochemicallyobserved inhibition of plant PS by pantoate, as well as of the alleviation of this inhibition by another sub-strate of the enzyme, ATP,...

Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... which does not appear to be the native conditionfor C-peptide [13]. The structural features of the aggre-gation properties of the amyloid precursor protein, aswell as of the opioid peptide dynorphin, ... of approximately 16 A˚,which is in agreement with the results of the NMR. The addition of SDS to the C-peptide sample leads toformation of larger objects, even at an SDS concentra-tion of ... widerange of spectroscopic techniques.Suitable methods for investigation of the secondarystructure and morphology of such structures, however,require the presence of large amounts of the oligomericstate,...

Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf

... a molecular mass of 16 309.7 Da, butthis matched none of the DNA-predicted proteins.MS ⁄ MS of these ions gave the C-terminal sequence of Fig. 5. These and all other peaks of that spectrum ... substitution;MS ⁄ MS will then show the substituent positions of dif-ferent isomers. A problem for MS ⁄ MS of either the peptides for the bottom-up approach or of the proteinsfor the top-down approach ... Tocheck for covalent modiﬁcations of the enzyme, the effect of the inhibitor on the molecular mass value of HAD was measured; instead of an adduct increase, orno change, the value had unexpectedly...

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