Báo cáo khoa học: Subunit sequences of the 4 · 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata Intramolecular evolution of the chelicerate hemocyanin subunits pot

... Subunit sequences of the 4 · 6-mer hemocyanin from the golden orb-web spider, Nephila inaurata Intramolecular evolution of the chelicerate hemocyanin subunits Anne Averdam, ... the 4 · 6-mer hemocyanin requires the stoichiometric association of all seven subunit types (4 · a, 2· b, 2· c ,4 d ,4 e ,4 f ,4 g), witheach subunit occupying a distinct position within the nativeoligomer ... leading to the b/c subunits emerged 545 ± 24 MYA, while the other subunit types emerged about 45 0 47 0 MYA.DiscussionStructure and subunit composition of N. inaurata hemocyanin The absence of subunit...

Báo cáo khoa học: Subunit composition of the glycyl radical enzyme p-hydroxyphenylacetate decarboxylase doc

... AJ 543 425 (hpdB), AJ 543 426 (hpdC)andAJ 543 427 (hpdA). Within the hpdB gene, nine nucleotideswere exchanged between the type strain and strain 630, butonly two of these replacements changed the ... allowin-frame cloning of the genes. While the BamHI sitesintroduced in the 3¢-UTR of the genes did not affect the resulting amino acid sequences, the introduction of NheIorNcoI sites next to the start ... established. The production of toxicfermentation end products may allow an ongoing suppres-sion of the endogenous microﬂora and therefore may play animportant role in the progression of the disease.The...

Báo cáo khoa học: Subunit S5a of the 26S proteasome is regulated by antiapoptotic signals pot

... transcriptional regulation of the humanS5a gene, we used PCR to isolate 1.2 kb of HeLagenomic DNA upstream of the ﬁrst exon of the ABCDEF24h 48 h 72h 96hRegulation of subunit S5a of the 26S proteasome ... factor of the Wntsignaling cascade, was present at position ) 244 of the promoter. The Wnt pathway regulates the ability of the proto-oncogene b-catenin to activate the transcrip-tion of speciﬁc ... of luciferase.In the FOPFlash reporter these three sites are mutated. Nor-malization of transcription from the TOPFlash reporter tothat from the FOPFlash reporter gives the net effect of the Wnt...

Báo cáo khoa học: Kinetic mechanism for p38 MAP kinase a A partial rapid-equilibrium random-order ternary-complex mechanism for the phosphorylation of a protein substrate potx

... aFEBS Journal 272 (2005) 46 31 46 45 ª 2005 FEBS 46 41extinction coefﬁcient (A280) of 52501 cm)1Æm)1, following the method of Gill and von Hippel [46 ]. The homogeneity of the protein was routinely ... Journal 272 (2005) 46 31 46 45 ª 2005 FEBS 46 43Murcko MA & Su MS (1997) The structural basis for the speciﬁcity of pyridinylimidazole inhibitors of p38MAP kinase. Chem Biol 4, 42 3 43 1.11 Pargellis ... for the second-order rate con-stant of ka¼ 10 4 m)1Æs)1for the binding of MgATP top38 MAPKa gives a rate constant for the dissociation of MgATP from the enzyme of k-a¼ 3.5 s)1, if the...

Báo cáo khoa học: "Extracting Sequences from the Web" pptx

... lever-aging the functionality and density properties of sequences as done in our SEQ system.286Pattern Example the ORD the fifth the RB ORD the very first the JJS the best the RB JJS the very best the ... presidents of the UnitedStates or the most common causes of deathin the U.S. SEQ leverages regularitiesabout sequences to extract a coherent set of sequences from Web text. SEQ nearlydoubles the ... given the sentence “With help from his father, JFK was elected as the 35th Pres-ident of the United States in 1960”, SEQ finds the candidate sequences with names “President”,“President of the...

Báo cáo khoa học: Weak organic acid stress inhibits aromatic amino acid uptake by yeast, causing a strong inﬂuence of amino acid auxotrophies on the phenotypes of membrane transporter mutants ppt

... lowers the intracellular levels of benzoate and fluoresceinby catalysing an active efflux of these compounds from the cell [2 ,4] . The inhibitory effects of different organic acids oncells of the ... Sal1-digested and cloned into the SalI site located between the ADH1 promoter and termi-nator of expression vector pAD4M [ 14] . The correct DNAsequence of the TAT2 insert of the resulting vector wasconfirmed ... either in the absence or the presence of acetate stress. The trp5D mutant is clearly muchmore sensitive than the wild-type to the inhibitory effects of acetate under these conditions (Fig. 4A,B)....

Báo cáo khoa học: and protein bilinear indices – novel bio-macromolecular descriptors for protein research: I. Predicting protein stability effects of a complete set of alanine substitutions in the Arc repressor ppt

... )1.38 36 SA 44- st11 46 .3 42 .1 4. 1913 KA2-st6 58.7 58 .4 0.26 37 NA29-st11 45 .3 45 .0 0.32 14 QA9-st6 58 .4 57.5 0. 94 38 VA33-st11 44 .1 47 .6 )3. 54 15 GA3-st6 58.1 59.0 )0. 94 39 EA48-st11 43 .2 46 .0 )2.7916 ... 3 .40 0.69 3. 64 3.91 43 RA31-st11 3 .40 1.11 2.52 2 .42 44 MA42-st11 3.60 0.75 2.36 3 .48 45 SA32-st11 3.80 0.50 Outlier 46 YA38-st11 3.80 1.51 2.65 3.76 47 WA 14- st11* 4. 00 4. 27 4. 80 5.02 48 RA40-st11 4. 60 ... 1.36 14 QA9-st6 58 .4 60.3 1.92 2.29 38 VA33-st11 44 .1 48 .7 4. 56 5. 04 15 GA3-st6 58.1 62.1 4. 02 4. 47 39 EA48-st11 43 .2 47 .2 3.95 4. 5816 MA1-st6* 58.0 54. 8 )3.16 ) 40 LA12-st11 42 .3 39.8 )2 .49 )3.1217...

Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc

... requires furtherinvestigation. In light of the apoptotic effect of PPARcin adipocytes, we conclude that PPARc is one of the key regulators involved in the physiology of the adipo-cyte, from its ... and30870559 from the China National Nature SciencesFoundation, 2006CB910703 from the Ministry of Sciences and Technology of China, and 07dz05907 from the Committee of Sciences and Technology of Shanghai.References1 ... accepted 24 November2009)doi:10.1111/j.1 742 -46 58.2009.075 14. xAlthough thiazolidinediones (TZDs) are potent promoters of adipogenesisin the preadipocyte, they induce apoptosis in several other...

Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf

... determined the effect of AG 147 8on the levels of MAPK phosphatases MKP-1 andMKP-2. As shown in Fig. 4B, AG 147 8 decreased the expression of the MKP-1 protein. As for the MKP-2protein, however, AG 147 8 ... examined the effect of AG 147 8 on the viabil-ity of human NSCLC cell line PC-9. Treatment of the cells with AG 147 8 markedly suppressed the cell viabil-ity, as determined by the results of a colorimetric ... AG 147 8 induced JNK activation, weincubated PC-9 cells in the presence of AG 147 8 forseveral periods, and then prepared cell lysates from these cells to determine the phosphorylation of MKK4and...

Tài liệu Báo cáo khóa học: A multi-protein complex containing cold shock domain (Y-box) and polypyrimidine tract binding proteins forms on the vascular endothelial growth factor mRNA Potential role in mRNA stabilization pptx

... by PCR from the pfVEGFconstruct [15], into pGEM4Z (Promega). The pGEM 44, 46 and 47 constructs contain, respectively, mouse VEGF5¢-UTR sequences +1 to +325, +46 1 to +727 and +735 to+10 14 (relative ... composed of the entire VEGFmouse 5¢-UTR (+1 to +10 14) , the coding region for the 1 64 aminoacidformofVEGFandtheVEGF3¢-UTR ( +4 to+2195). The major polyadenylation site is at +1861 [59]. AFig. 2. The ... majorcomplex (CCV1) formed on the V1 probe and migrated in asimilar position to the CC44a complex on the 44 RNAprobe (Fig. 2B). To verify that the CC44a and CCV1complexes were the same, complexes were...

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