Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections ppt
Báo cáo khoa học: Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections ppt
... (Eur. J. Biochem. 270) 3407 Pherokine-2 and -3 Two Drosophila molecules related to pheromone/odor-binding proteins induced by viral and bacterial infections Laurence Sabatier1,*, Emmanuelle Jouanguy1,*†, ... wasanalyzed by HPLC and the molecule induced by the viral infection was detected by MALDI-TOF MS (Fig. 2B). Themolecule was purified to homogeneity by gel permeation and reversed-phase chromatography and ... monitored by absorbance at 214 nm and the fractions were hand-collected and analyzed by MALDI-TOF MS. Threepurified fragments were submitted to automated Edmandegradation on a pulse liquid automatic...
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Báo cáo khoa học: Maturation of Pichia pastoris-derived recombinant pro-Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite doc
... a ndDer p 1 in Pichia pastoris was recently r eported by Yasuharaet al.[23]andBestet al. [24,25], respectively. Maturation ofrpro-Der f 1 was shown to be induced by dialysis againstpH 4.0, ... ature and pro-Der p 1 were obtained by PCR (Table 1). Subse-quently, they were cloned into pPICZaA(andpGAPZaA)in-frame with the s ecretion peptide (Table 1). DNAsequences were d etermined by automated ... silver-stainedaccording to the ExcelGel pr otocol (Amersham PharmaciaBiotech, Uppsala, Sweden). Western blotting was per-formed by transferring the proteins on to nitrocellulosemembrane as described by the...
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Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf
... 4·-RNase HI and 5·-RNaseHI proteins by 27.0, 14.8 and 7.0 kJÆmol)1 and 3.1, 1.7 and 0.8 m, respectively. Thus, the stabilities of the5·-RNase HI and 6·-RNase HI proteins against urea- induced ... residues 29, T34 and E131 are shown. The hydrogen bonds betweenN29 and Oc and T34 and Oc, and between N29 and Nd and E131 and Oe2, in the wild-type protein are shown as green broken lines, and the ion ... Lys90 and Lys89 and ⁄ or between Lys90 and Arg97 are eliminated.The Arg97 fi Gly mutation stabilizes the wild-type and 4·-RNase HI proteins by 5.4 and 3.4 °C, respec-tively, in Tm, and 3.5 and...
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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx
... 5¢-GAGAATCCAGCTTCTTTCCC-3¢ and antisense, 5¢-GGCGACACTGTATGAATTGC-3¢);SENP2 (sense, 5¢-AACAGTCTCTACAATGCGGCCA-3¢ and antisense, 5¢-CCGTGTTCCATTACAAGCAGAA-3¢);SAE1 (sense, 5¢-GACCTGCTTCCCGATGACTTT-3¢ and antisense, ... 5¢-TTCCTCCAACCACAGCACATAC-3¢); UBC9(sense, 5¢-CAACAAAGAACCCTGATGGCACGA-3¢ and antisense, 5¢-GCATCCGTAGCTTGAACAAGCCTC-3¢);PIAS3 (sense, 5¢-ACTGCAGGGACCCTGCTACA-3¢ and antisense, 5¢-CTTGATCAGTGCTCGGGAATG-3¢); PIASxa(sense, ... breastadipose and may play a role in breast tumor develop-ment [13,14].Nuclear hormone receptors are typically ligand-acti-vated transcription factors and their activities aremodulated by coactivators...
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Báo cáo khoa học: Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals pptx
... conserved sequence signatures to early deuterosto-mes [12,13,21–23], and indeed to lophotrochozoans,prebilaterans and even unicellular eukaryotes, althoughit appears to have been lost throughout ... mediterranea, and the sea anemoneNematostella vectensis. However, human pFGE and also pFGE from 20 further mammalian species (fromvarious primates to squirrel, bat, dolphin, sloth and wallaby) carry ... pFGE⁄ FGEheterodimerization and ternary complex formationwith sulfatases was reported by Zito et al. [24] to serveas a regulatory mechanism for FGE activity. We have to point out that, although...
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Báo cáo khoa học: Secondary structure conversions of Alzheimer’s Ab(1–40) peptide induced by membrane-mimicking detergents pdf
... dodecyl sulfate to a dilute aqueous solution, Ab(1–40) isconverted to b-sheet, as observed by CD at 3 °C and 20 °C. The transitionis mainly a two- state process, as revealed by approximately ... structured state and conversion to b-sheet and then to a-helix are evident. The CD intensities at thiswavelength allowed us to compare the detergentsecondary structure induction at 3 °C and 20 °C(Fig. ... prep-aration and are not detectable by NMR or CD. At1.4 mm SDS (D⁄ P = 14), the IR band indicating ab-sheet is transformed into two shoulders, which mightrepresent the seeds and the b-sheet...
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Tài liệu Báo cáo khoa học: Activation loop 3 and the 170 loop interact in the active conformation of coagulation factor VIIa pdf
... (A) and FVIIa (B) with sTF. The curves represent analyte injected at 20, 40,80, 160 and 320 nM, respectively, from bottom to top.Hydrogen bonds involving Gly372 in factor VIIa E. Persson and ... TF-interactive helix and the 170 loop (red), and the covalently attached inhibitor fFR-cmk (purple). The hydro-gen bonds from Gly372(223) to Arg315(170C) and Ser-333(185) areindicated by dotted lines, ... substrates by mutant (s) and wild-typeFVIIa (d) are shown. The data obtained with S-2288 and S-2366were fitted to the Michaelis–Menten equation, and non-linearregression (using GraFit) was used to...
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![Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc](https://media.store123doc.com/images/document/14/br/vp/small_vpv1392870032.jpg)
Tài liệu Báo cáo khoa học: Specific targeting of a DNA-alkylating reagent to mitochondria Synthesis and characterization of [4-((11aS)-7-methoxy-1,2,3,11a-tetrahydro-5H-pyrrolo[2,1-c][1,4]benzodiazepin-5-on-8-oxy)butyl]-triphenylphosphonium iodide doc
... [30,31].Accumulation of mitoDC-81 by isolated mitochondriaAn ion-selective electrode sensitive to mitoDC-81 wasconstructed in order to determine whether mitoDC-81 wasaccumulated by energized mitochondria ... electrode to cation concentra-Fig. 2. Uptake of mitoDC-81 by energized mitochondria. An ion-selective electrode was used to measure the uptake of mitoDC-81 intorat liver mitochondria. To calibrate ... to protein thiols, enabling it to be detected by anti-TPP serum [35]. If mitoDC-81 binds to mtDNA itwill also be retained within mitochondria after fixation and should be visible by immunocytochemistry...
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Báo cáo khoa học: Isoquinoline-1,3,4-trione and its derivatives attenuate b-amyloid-induced apoptosis of neuronal cells pdf
... cold- and ischemia -induced apopto-sis in donor livers and reduces sinusoidal endothelialcell apoptosis and caspase-3 activity by 94%. It hasrecently been granted orphan drug status for liver and ... caspase-3 inhibitors can blockPC12 apoptosis induced by Ab(25–35) and that theyare not toxic for PC12 cells.The effect of compound 1 on cytotoxicity induced by Ab was assessed using the conventional ... apparently blocked cellapoptosis rate at 28 lM induced by the neu-rotoxicity of Ab(25–35) without toxicity to PC12 cells, and Ac-DEVD-CHO protectedapoptosis at 2 lM. (D) Caspase-3 activityof PC12...
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Báo cáo khóa học: S-(2,3-Dichlorotriazinyl)glutathione A new affinity label for probing the structure and function of glutathione transferases potx
... against toxicxenobiotics and byproducts of oxidative metabolism. Inaddition to their catalytic activities, plant GSTs are alsoinvolved in the response to different biotic and abioticstresses, and ... A˚2,andtheB-factors of S atoms are 38.14 A˚2 and 79.30 A˚2, respect-ively. It is therefore reasonable to propose that conform-ational changes and changes in dynamics may alsocontribute to ... determination of total carboxyl and primaryamino groups, respectively. The same treatment was alsoapplied to the unmodified GST I, as a control. Totalcarboxy and primary amino groups in the modified and unmodified...
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