Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
... yellow, acarviose transferase; pink, maltogenic a- amylase; blue, a- amylases from Bacillus and actinomycetes; light blue, a- amylases from fungi and yeast; green, maltotetraohydrolases and maltopentaohydrolase. ... (SBD): a- amylase, maltotetraohydrolase, maltopentaohydrolase, maltogenic a- amylase, acarviose transferase, and cyclodextrin glu-canotransferase (CGTase). Such enzymes are multidomainproteins and ... specificity, and taxonomy of the a- amylase family members containing a C-terminal starch-binding domain Sˇtefan Janecˇek1, Birte Svensson2 and E. Ann MacGregor31Institute of Molecular Biology,...
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Tài liệu Báo cáo khoa học: Multiple promoters regulate tissue-specific alternative splicing of the human kallikrein gene, KLK11⁄hippostasin ppt
... 5¢-TCATGCTGGTTGAGACTGGA-3¢ and 5¢-CCAGGTGTGGAGCAAGGCAG-3¢) and a 938 bp fragment of promoter 3 (primers, 5¢-CTCACAGCAGCCAGTAAGTG-3¢ and 5¢-ACTCACAGGCTCTGGGGCTG-3¢) were amplified using a Taq polymerase ... 5¢-GGACTCAAGAGAGGAACCTG-3¢; F1b, 5¢-CTGCCTTGCTCCACACCTG-3¢; F1c, 5¢-CTGCCGTCTCCGCCGCCACT-3¢; FU, 5¢-TCAAGCCCCGCTACATAGTT-3¢; and R3, 5¢-AGGAACCAAACACCAAGTGG-3¢(Fig. 2A) .Luciferase promoter assayHuman ... raised against KLK11 was the same sizeas that of the translational product by isoform 2mRNA (Fig. 4B). The product by isoform 1 mRNAwas smaller than the isoform 2 product. The multiplebands may...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... CTCTTTGATGACAGAAGTGCCT-3¢Syndecan-4 5¢-85 GAGTCGATTCGAGAGACTGA-3¢ 3655¢-450 AAAAATGTTGCTGCCCTG-3¢Glypican-1 5¢-566 GAATGACTCGGAGCGTACACTG-3¢ 4885¢-1054 CCTTTGAGCACATTTCGGCAA-3¢P450 aromatase 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ ... 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ 2895¢-1821CAAGGGTAAATTCATTGGGCTTGG-3¢b-actin 5¢-2350 ACAGACTACCTCATGAAGAT-3¢ 6655¢-3222 AGCCATGCCAAATGTCTCAT-3¢Fig. 1. Dose-related eect of bFGF on FSH-stimulated ... USA).DNA quanti®cation The DNA content of the cell layer at the end of incubationwas quanti®ed by the method of West et al .[31].Aftersolubilization of the cell layer in 1MNaOH and s ubsequentneutralization...
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Báo cáo khoa học: Ribonuclease H: properties, substrate specificity and roles in retroviral reverse transcription ppt
... of the associated polymerase domain. In the internalcleavage mode, the RNases H behave as typical endo-nucleases and cleave the RNA along the length of a DNA ⁄ RNA hybrid substrate in the absence ... retroviral RNases H. DNA ⁄ RNAhybrids are drawn with RNA strands in red and DNA strands inblack. In the internal cleavage mode, the arrows mark the sites of cleavage along the length of the hybrid ... minus-strand synthesis, precisely generate the primer for the initiation of plus strands, facilitate the initiation of plus-strand synthesis and removeboth plus- and minus-strand primers after they...
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Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot
... ectodomains, C-terminal fragments and intracellular domains (ICDs). One of the proteases that is known to cleaveAPP and that is essential for generation of the amyloid b-protein is the b-site APP-cleaving ... that the APP family of proteins have a role in cell–cell and cell–matrix adhesion, and that they can form both cis and trans homodimers and heterodimers [15,16]. In addi-tion, the APP family of ... expressed, and its subcellular localization and dimerization prop-erties are different from those of APP and APLP2 [16].Given the similarities between processing of Notch and of the APP family of proteins,...
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Báo cáo khoa học: Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic antigen absent from nonspecific cross-reacting antigen doc
... hamster ovary cells. Hybridoma(Larchmt) 26, 1–9.9 Ismaili A, Jalali-Javaran M, Rasaee MJ, RahbarizadehF, Forouzandeh-Moghadam M & Memari HR (2007)Production and characterization of anti-(mucinMUC1) ... sev-eral groups have reported the isolation of single- domain antibodies (sdAbs) consisting of the variable domain of these heavy chain antibodies, also namedVHH [2]. These minimal antibody domains ... pancreas, stom-ach, thyroid and ovarian tumors. Nonspecific cross-reacting antigen (NCA or CEACAM6) is a highlyrelated member of the same CEACAM family. CEA and NCA polypeptides have extracellular...
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Báo cáo khóa học: Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II potx
... min at 30 °C, stopped by the addition of SDS/PAGE loading buffer, and analyzed on SDS/PAGEgels and by autoradiography. The separation of substratesfrom kinases after the kinase reaction was carried ... 1009lanes with samples that contained no substrate. Afterpreparing a graph of the intensity of the signal from the substrates only, we calculated the percentage of signal in the IIo and IIa bands. ... University of Guelph, Ontario, Canada The C-terminal domain (CTD) of the largest subunit of RNA polymerase II plays critical roles in the initiation,elongation and processing of primary transcripts. Theseactivities...
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Tài liệu Báo cáo khoa học: "Finding Synonyms Using Automatic Word Alignment and Measures of Distributional Similarity" pdf
... of a word into other languages found in parallel cor-pora are seen as the (translational) context of thatword. We assume that words that share transla-tional contexts are semantically related. ... >1.newspaper text (Algemeen Dagblad and NRCHandelsblad 1994/1995). The corpus was parsedautomatically using the Alpino parser (van derBeek et al., 2002; Malouf and van Noord, 2004). The result of ... experiments and finally the results of this approach.8695.2.1 Data and ResourcesMeasures of distributional similarity usually re-quire large amounts of data. For the alignmentmethod we need a parallel...
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Báo cáo khoa học: Staphylococcus aureus elongation factor G – structure and analysis of a target for fusidic acid pdf
... II and III, the only physical connec-tion between domains G and II and domains III, IV and V. In the S. aureus EF-G structure, this loop has a bent conformation, and packs against domain III and ... 666 and 670, within one b-strand of domain V, are also important for interdomain interac-tions. The N-terminal part of the strand interacts with the N-terminal part of helix CGin domain G, and ... Helgstrand M, Mandava CS, Mulder FA, Liljas A, Sanyal S & Akke M (2007) The ribosomal stalk bindsto translation factors IF2, EF-Tu, EF-G and RF3 via a conserved region of the L12 C-terminal domain. ...
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Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx
... complexes The S, Zn, Cd and Cu content of the Zn–, Cd– and Cu–CeMT preparations was analyzed by means of ICP-AES in a Polyscan 61E (Thermo Jarrell Ash, Franklin,MA, USA) spectrometer, measuring S at ... residue, the wild-type cDNA wasamplified using the same upstream primer as for the entirecDNA (5¢-CGGGGATCCATGGTCTGCAAG-3¢) but5¢-ACGCGTCGACCTAAGCAGCCTG-3¢ as the down-stream primer.All the PCR ... moietieswere obtained by PCR amplification with the primers:N-terminal fragment (residues 1–27), 5¢-CGTGGATCCATGGCTTGCAAGTGT-3¢ (upstream) and 5¢-GCTCGAGTCGACTTACTCACAACACTTGTC-3¢ (downstream) and C-terminal...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnchuyên đề điện xoay chiều theo dạngGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP