... variety of celltypes, including hepatocytes, to long-chain saturatedfatty acids (SFAs) led to increased expression of proin-flammatory cytokines, inhibition of insulin signaling,induction of endoplasmic ... Pharmigen, San Diego, CA, USA) binding andPI staining were performed according to [44]. Followingincubation with 1.0 lgÆmL)1annexin V and 4.0 lgÆmL)1PIfor 20 min, cells were analyzed by flow ... results that TAG synthesiswas initially hindered following stearate administrationand was completely interrupted at longer incubationperiods. Interestingly, the interruption of TAG synthe-sis...