... Juro Supplies,Lucerne, Switzerland) were added, and the slurry was incu-bated for 36 h at 6 °C on a rotating wheel. The beads werethen pelleted by centrifugation, and the supernatant con-taining ... kDa) and cata-lyze (232 kDa).AcknowledgementsWe would like to thank M. Linder and X. Lan Vu for their technical assistance, R. Brun of the Swiss Trop-ical Institute, University of Basel, for ... MD,USA) for providing expression plasmids for Vps27p-FYVE-GST fusion proteins, Peter Buetikofer (Instituteof Molecular Biology, University of Bern) for provi-ding phospholipids and valuable...