... reflectron mode by accumulat-ing data from 1800 laser shots per spot. Data acquisition wasperformed by flex control 3.0.101.1, which was controlled by warplc 1.1 (Bruker Daltonik). Ion signals with signal-to-noise ... were incubated for 18 h in DMEMsupplemented with 0.5% fetal bovine serum, washed twice with NaCl ⁄ Pi, and incubated in serum-free DMEM with orwithout elastin peptides (200 lgÆmL)1) for ... number: P15502-2) with MMP-7,MMP-9, and MMP-12. Cleavage sites are indicated by triangles (MMP-7, red; MMP-9, green; MMP-12, black), and all regions covered by peptides are labeled with solid lines...