Báo cáo khoa học: Transport of L-arginine and nitric oxide formation in human platelets pdf

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... line), in the presence of 100 lM neurotoxin (•, dotted line), in thepresence of 200 lM neurotoxin ( , dottedline) and in the presence of neurotoxin and 50 lM dopamine (h, dashed line).Modulation ... Thisconﬁrmed the formation of SDS-insoluble aggregates of a-synuclein on incubation.Effect of MPTP and MPP+on the aggregationpattern of a-synucleina-Synuclein was incubated with 100 and 200 lmMPTP ... abetter understanding of the role of various interactingcomponents. The formation of dopamine–quinoneadducts (because of auto-oxidation of the neurotrans-mitter), especially dopaminochrome, with...

Tài liệu Báo cáo khoa học: Mechanisms of obesity and related pathologies: Androgen deﬁciency and endothelial dysfunction may be the link between obesity and erectile dysfunction pptx

... hormone-binding-globulin, leptin,insulin and insulin resistance in obese men. ArchAndrol 52, 355–361.19 Wannamethee SG, Shaper AG, Morris RW &Whincup PH (2005) Measures of adiposity in the ... serve asan endocrine disrupter and in uence the endocrinemilieu by reducing the levels of luetinizing hormone(LH) and testosterone resulting in hypogondoropic-hypogonadism. The increased aromatase ... plasma androgen levels [14–19]. In thisminireview, we discuss the link between obesity and ED by focusing on the potential pathophysiologicallinks including obesity-related androgen deﬁciency and obesity-induced...

Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt

... subheading and then select the Proteomics link).One of the proteins of interest to the Cell MigrationConsortium is paxillin, a scaffolding protein involved in focal adhesions [9]. Shown in Fig. ... building blocks of eukaryotic chromatin[15]. A host of PTMs reside on the N-terminal tails of the histones, and this array of PTMs has been welldocumented and includes monomethylation and Fig. ... 6271dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitina-tion of Lys, and phosphorylation of Ser and Thr [16–18]. Combinations of these modiﬁcations...

Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc

... determinedusing serial dilutions of samples (Fig. 2). Linearityconsisting of continuous signal increase and of repro-ducible glycoprotein patterns was determined in therange between 4 and 10 ... Comparison of the PrPCbanding patterns of various speciesdetected by amino- and carboxyl-binding antibodies. After immuno-blotting, PrPCproteins were detected using N- or C-terminal bindingantibodies. ... C-terminal binding antibody wasused. In contrast, a protein band at the size of thenonglycosylated full-length PrPC of humans, sheep and cattle was highly abundant when using C-terminalbinding...

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... charged amino acid sidechains, R397 and R120, are also involved in fosfomycinbinding, and in view of the multitude of interactions, it wasassumed that deletion of a single interaction would not ... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins.However, two other positively charged amino ... Hence, themechanism of binding of fosfomycin by reinforcement of aninitial binding site (mainly K22 and R397) through recruit-ment of secondary binding partners located in a ﬂexibleloop (R120)...

Tài liệu Báo cáo khoa học: "Order of Subject and Predicate in Scientific Russia" pptx

... understanding," since " ;in the field of scientific writ- ing, Russian sentence structure is definitely close to English — much closer than is normal for other forms of Russian ... prose. " In support of this statement, Harper quotes certain figures: "From a sample of 1, 528 sentences containing a subject and verb: Subject before verb: 81% of all occurrences ... tion, and the following types of fillers were found in the predicate spot: verb, adjective, noun, prepositional phrase, and various types of impersonal expressions.3 Sentences containing...

Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafﬁcking pdf

... five amino acids of theextracellular domain and devoid of any tag was notfunctional [27], while a mutant containing four amino acids of the ectodomain and including a rhodopsine tag wasfound ... periods of times (0–60 min)at 37 °C. The concentration of the biotinylated Ig remainingon the cell surface was then quantified by measuring thebinding of [125I]Streptavidin. In the absence of ... presence of a seven transmembrane domain and alarge extracellular domain involved in high affinity hormonebinding. These three receptors are mainly coupled to Gs,leading to the activation of the...

Báo cáo khoa học: Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast ppt

... mutationalanalysis of Lsm1p showing the importance of residuesproposed to be involved in RNA binding and complex formation, and of the C-terminal region for the func-tional competence of the Lsm1–7p ... accumulates in cyto-plasmic foci in 5–20% of cells under stress condi-tions (Fig. 5B), suggesting that the N-terminaldomain of Lsm1p is sufﬁcient in combination withthe Sm and C-terminal domains of ... C-terminal domain deleted is consistentwith this (Figs 4 and 5). Hybrid proteins carrying theN-terminus of one protein and the Sm domain of theother localize according to the N-terminal contri-bution:...

Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt

... motif present in DptF is GLDSV,indicating that this putative ACP domain is one of thefew ACPs in which valine replaces isoleucine (I) orleucine (L) in the conserved sequence. To determinewhether ... overexpressed in Escherichia coliBL21(DE3). The C-terminally His6-tagged and N-ter-minally thioredoxin-fused protein was purified, yielding4.4 mgÆL)1 of culture. The identity of the protein wasconfirmed ... of DptE, weexpressed dptF using the pQTev vector in E. coli and purified the resulting ACP as an N-terminal His7 fusionprotein (Fig. 3) with a yield of 9.5 mgÆL)1 of culture.The identity of...

Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces signiﬁcant structural changes doc

... and ⁄ orchanges of the spectral resonances were observed in theNOESY spectrum of the mixture, an interaction of thetwo single domains and the formation of one singleCu(I)-containing domain ... that zinc was requiredfor the in vivo and in vitro folding of the two domains of copper MTs [27]. Replacement of Zn(II) by Cu(I)led to the proposal of the formation of Cu,Zn-MTintermediates and ... MT-1 and MT-2 are composed of the N-terminal b- and the C-terminal a-domain. Theyare predominantly isolated containing zinc or cadmiumexclusively bound to cysteine thiolates. The nine cys-teine...

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