Báo cáo khoa học: Involvement of the V2 receptor in vasopressin-stimulated translocation of placental leucine aminopeptidase/oxytocinase in renal cells pdf
... with the PRAD domain
of ColQ indicates that the WAT domain of the
C. intestinalis AChE is capable of organizing the sub-
units into a tetramer through interaction with the
PRAD domain of ColQ. The ... from
C. intestinalis expressed in vitro and native
enzyme expressed in vivo indicates the enzyme
is AChE
To determine the nature of the cholinesterase activity
o...
... impair or limit the ApoE interaction
with these targets.
The binding and the consequent shielding of ApoA-I
and ApoE by Hpt are expected to in uence these
apolipoproteins in their interaction not ... above.
Nonspecific binding of Hpt was determined by omitting
ApoE from the coating step. Absorbance values were con-
verted to percentage of Hpt binding in the absence of P2...
... confirm the integrity of the product, and
this process was carried out for each of the mutant variants.
Protein determination
Protein concentrations were determined using the bicinchon-
inic acid ... conditions. These data indi-
cate that the R514Q mutation increases angiotensin II
hydrolysis by enhancing the maximal level of activity,
but not by altering the substrate-bind...
... Involvement of the V
2
receptor in vasopressin-stimulated
translocation of placental leucine aminopeptidase/oxytocinase
in renal cells
Shinako Masuda
1
, Akira Hattori
1
, ... the
presence of a minor population of AQP-2-containing
vesicles.
Taken together, we suggest the biological relevance of
our findings as follows. The binding of AVP to V
2
recepto...
... mechanism of
GAPDH could be essential for the binding of CP12. The
presence of this region at the interface of GAPDH and
CP12 could also explain the kinetic changes o bserved for the
binding of the ... involved in the interaction with the
other partners of the GAPDH–CP12–PRK complex. The
model of wild-type GAPDH from C. rein hardtii, like the
structure o...
... abolished
the formation of Complex A, indicating that the p65
subunit and members of the RAR family are involved
in the DNA–protein Complex in TE671 cells. Intrigu-
ingly, along with the abolition of ... activation
involves the p50 ⁄ p65 heterodimer, which interacts with
the jB site and the CRE of the promoters. Being the
active partner of the heterodimer...
... repre-
sent the last subunits incorporated into the bc
1
com-
plex. On the other hand, the absence of Qcr9p in the
DQCR9 ⁄ DQCR10 strain prevented the binding of ISP
(Fig. 4B). Interestingly, in the ... comparable amounts in
both yeast strains. Therefore, the reason for the disap-
pearance of the bc
1
dimer in the yeast strain in which
Qcr10p is missing r...
... conformation, the
SH2 domain binds to pY
i
on the C-terminal tail, and
the Src homology 3 (SH3) domain binds to the linker
between the SH2 and kinase domains at the back of
the small lobe, preventing the ... by presenting the pulse of Src activity in the plane
of the inactive and active fractions, s
i
and s
1
. If the
duration of the stimulus exceeds the...
... composition, indicating that the peptide is of
the correct sequence and free of contaminating pro-
teins. Five cycles of N-terminal sequencing confirmed
the expected residues including the presence of methio-
nine ... : 2000 in blocking solution for 30 min. Cells were
rinsed in NaCl ⁄ P
i
several times and incubated in blocking
serum containing anti-mouse IgG (Vectastai...
... is the
lack of domain B protruding out of the TIM barrel in
the place of loop 3 connecting the b3-strand to the
a3-helix [9,21]. Sharing domain B by rBATs and
GH13 enzymes, and especially the ... domain,
including domain B and the C-terminal domain C for
rBAT proteins (669 residues on average); (b) the cata-
lytic TIM barrel domain, including domain B and the
C-termi...