Báo cáo khoa học: "Joint Identification and Segmentation of Domain-Specific Dialogue Acts for Conversational Dialogue Systems" doc
Báo cáo khoa học: "Joint Identification and Segmentation of Domain-Specific Dialogue Acts for Conversational Dialogue Systems" doc
... LinguisticsJoint Identification and Segmentation of Domain-Specific Dialogue Acts for Conversational Dialogue SystemsFabrizio Morbini and Kenji SagaeInstitute for Creative TechnologiesUniversity of Southern ... 7.5 (with a maximum of 34, and minimum of 1), and the average length of utterances with multiple dialogue acts is 15.7 (max-imum of 66, minimum of 2). To give a better idea of the dataset used ... single dialogue act. Multiple is for utterances annotated with more thanone dialogue act, and Overall indicates the performanceover the entire set. P stands for precision, R for recall, and F for...
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Báo cáo khoa học: Molecular identification and expression study of differentially regulated genes in the Pacific oyster Crassostrea gigas in response to pesticide exposure doc
... regulation of specific herbicide-regulated gene expression.Results Identification of herbicide-regulated genesTwo forward and reverse SSH libraries were madefrom pooled digestive glands and gills of ... and digestive gland of eight control and eight exposed oysters at days 0, 7, 15, 21 and 30 days using a method based on extraction in guani-dium isothiocyanate [80]. For each oyster, 50 lg of ... cycles) for each geneexcept for the 28S, where 25 cycles were used to avoid bandintensity saturation for optical determination. Band intensi-ties were quantified using the gene profiler software...
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Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot
... Tuovinen for the fruitful criticalreading of the article. J.B. thanks Mark Dopson and Siv Sa¨a¨fforpractical help, Arunas Leipus for help in protein chromatography and Henrik Larsson for helpful ... production of p-nitrophenol was measuredspectrophotometrically at 405 nm. One unit of the acidphosphatase activity was defined as the amount of proteinwhich catalysed the formation of 1 lmol of p-nitrophenol ... line). The retention time of tetrathionate was 9 min.276 Z. Bugaytsova and E. B. Lindstro¨m(Eur. J. Biochem. 271) Ó FEBS 2003evidence for the formation of thiosulfate and pentathionate(data...
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Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
... expression vector under the control of the T7promoter, and coexpression of oraE and oraS genes wascarried out in E. co li. Meanwhile, the extent of theinvolvement of AdoCbl or PLP in the in vivo foldingprocess ... temperatures:95 °C for 30 sec, 50 °C for 1 min, and 72 °Cfor4min.Finally, the reaction was maintained at 72 °Cfor5min.The PCR products were gel-purified, restricted with NcoI and BamHI, and ligated ... measuring the apparent Kmvalue of D-ornithine at 25 , 50, 100, 200 and 400 lM of the inhibitor. For the measurement of the activity of the substrateanalogues, an HPLC and NMR-based method was devel-oped....
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Báo cáo khoa học: Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein pot
... in ovary, testis and brain, the most significant expression of the WFIKKNgene is observed in adult pancreas, liver and thymus.In view of the presence of WAP-, Kazal-, Kunitz- and NTR-modules ... (Bubendorf, Switzerland). p-Nitrophenyl-p-guanidinobenzoate was a product of Fluka (Buch,Switzerland).Cloning and expression of the second Kunitz-typeprotease inhibitor module of human WFIKKN ... Ó FEBS 2003The presence of both b-strands and a-helices inWFIKKN-KU2 is consistent with the fact that all homo-logues of WFIKKN-KU2 are known to contain b-strands and a-helices in equivalent...
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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
... of formation of these two states should be almostequal. The lifetime of each of the conformationalstates, and therefore the energy barrier between them,is ... were obtained for more than 99% of the1H,13C and 15N atoms of the protein backbone, and for more than 78% of the side chain atoms.The main set of backbone u and w dihedral angles wascalculated ... most of the residues of the protein rigid corecould be successfully fitted using models 1 and 2. For a fewresidues of the minidomain (330–332, at the beginning of the central b-strand) and residues...
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Tài liệu Báo cáo khoa học: Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS docx
... 25 °C.Extraction and preparation of mycelia for MALDI-TOF analysisA few micrograms of fungal mycelia were suspended inacetonitrile ⁄ methanol ⁄ water (1 : 1 : 1), and 1 lL of the sus-pension ... mass shifts of 8 Da of reduced and unreduced forms, the analysis of knockout mutants, and comparison with protein databases. Strain-speci-fic processing was observed in the case of Hypocrea jecorina ... potential of intact-cell MALDI-TOFMS (ICMS). Initial applications of ICMS on fila-mentous fungi have demonstrated characteristic sets of ions for strain identification in mycelia [4–8] and spores...
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Báo cáo khoa học: Proteomic identification of all plastid-specific ribosomal proteins in higher plant chloroplast 30S ribosomal subunit PSRP-2 (U1A-type domains), PSRP-3a/b (ycf65 homologue) and PSRP-4 (Thx homologue) doc
... for amplifications of 3¢-portions of Psrp-2 (P2-1/PR) and Psrp-3 (P3-1/PR), and 50–57 °C for amplification of 3¢-portion of Psrp-4 (P4-1/PR). Here, for example, P2-1/PR stands for PCR amplified ... to the 5¢ and 3¢ ends of cDNA inserts (see Materials and methods for furtherinformation), and both strands of the cDNAs obtained werecompletely sequenced. The nucleotide sequences of thecDNAs ... (general name for the organelle, of whichchloroplast is one of the differentiated forms) have theirown genome, and employ for gene expression a transcrip-tion-translation system composed of both...
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Báo cáo khoa học: Structural identification of ladderane and other membrane lipids of planctomycetes capable of anaerobic ammonium oxidation (anammox) pptx
... of ether bonds with HI and subsequent reduction of the formed iodides was performed as described previ-ously [18].Isolation of ladderane lipids For isolation of lipids the extract (54 mg) of ... University of Amsterdam, the Netherlands3 Department of Microbiology, Institute of Water and Wetland Research, Radboud University Nijmegen, the NetherlandsRecently, identification of the lithotroph ... Strous3 and Mike S. M. Jetten31 Royal Netherlands Institute for Sea Research (NIOZ), Department of Marine Biogeochemistry and Toxicology, Texel, the Netherlands2 van ‘t Hoff Institute for Molecular...
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Báo cáo khoa học: Molecular identification of monomeric aspartate racemase from Bifidobacterium bifidum pptx
... lLof8Murea and incubated at 37 °C for 1 h. To the solution, 60 lL of 0.2MTris/HCl buffer (pH 9.0) and 5 pmol of lysylendopeptidase were added, and the m ixture was incubated at37 °C for ... wasdissolved in the standard buffer (pH 6.5) and dialyzedovernight against the same buffer. The enzyme s olutionwas kept at 60 °C for 30 min in the presence of 0.1MD-aspartate, and the formed p recipitate ... enzyme was incubated at 45 °C for 10 h. The reactionwas terminated by a ddition of 50 lLof2MHCl. Afterneutralization of the reaction mixture, it was incubated at25 °C for 2 min with a 0.3Mborate...
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